Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1136/bjo.2003.017731 http://repositorio.unifesp.br/handle/11600/27599 |
Resumo: | Background/aims: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. the purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.Methods: Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.Results: Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).Conclusion: Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes. |
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Repositório Institucional da UNIFESP |
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3465 |
spelling |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytesBackground/aims: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. the purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.Methods: Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.Results: Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).Conclusion: Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes.Wills Eye Hosp & Res Inst, Div Res, Philadelphia, PA 19107 USAUniversidade Federal de São Paulo, UNIFESP, São Paulo, BrazilUniv Nottingham, Dept Ophthalmol, Nottingham NG7 2RD, EnglandNIH, Bethesda, MD 20892 USAUniv São Paulo, BR-05508 São Paulo, BrazilFund EJ Zerbini, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, São Paulo, BrazilWeb of ScienceB M J Publishing GroupWills Eye Hosp & Res InstUniversidade Federal de São Paulo (UNIFESP)Univ NottinghamNIHUniversidade de São Paulo (USP)Fund EJ ZerbiniGomes, José Álvaro Pereira [UNIFESP]Dua, H. S.Rizzo, Luiz V.Nishi, Mauro [UNIFESP]Joseph, A.Donoso, L. A.2016-01-24T12:34:16Z2016-01-24T12:34:16Z2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion280-285http://dx.doi.org/10.1136/bjo.2003.017731British Journal of Ophthalmology. London: B M J Publishing Group, v. 88, n. 2, p. 280-285, 2004.10.1136/bjo.2003.0177310007-1161http://repositorio.unifesp.br/handle/11600/27599WOS:000188302800032engBritish Journal of Ophthalmologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-26T10:22:12Zoai:repositorio.unifesp.br/:11600/27599Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-26T10:22:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
title |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
spellingShingle |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes Gomes, José Álvaro Pereira [UNIFESP] |
title_short |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
title_full |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
title_fullStr |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
title_full_unstemmed |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
title_sort |
Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes |
author |
Gomes, José Álvaro Pereira [UNIFESP] |
author_facet |
Gomes, José Álvaro Pereira [UNIFESP] Dua, H. S. Rizzo, Luiz V. Nishi, Mauro [UNIFESP] Joseph, A. Donoso, L. A. |
author_role |
author |
author2 |
Dua, H. S. Rizzo, Luiz V. Nishi, Mauro [UNIFESP] Joseph, A. Donoso, L. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Wills Eye Hosp & Res Inst Universidade Federal de São Paulo (UNIFESP) Univ Nottingham NIH Universidade de São Paulo (USP) Fund EJ Zerbini |
dc.contributor.author.fl_str_mv |
Gomes, José Álvaro Pereira [UNIFESP] Dua, H. S. Rizzo, Luiz V. Nishi, Mauro [UNIFESP] Joseph, A. Donoso, L. A. |
description |
Background/aims: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. the purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.Methods: Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.Results: Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).Conclusion: Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-02-01 2016-01-24T12:34:16Z 2016-01-24T12:34:16Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1136/bjo.2003.017731 British Journal of Ophthalmology. London: B M J Publishing Group, v. 88, n. 2, p. 280-285, 2004. 10.1136/bjo.2003.017731 0007-1161 http://repositorio.unifesp.br/handle/11600/27599 WOS:000188302800032 |
url |
http://dx.doi.org/10.1136/bjo.2003.017731 http://repositorio.unifesp.br/handle/11600/27599 |
identifier_str_mv |
British Journal of Ophthalmology. London: B M J Publishing Group, v. 88, n. 2, p. 280-285, 2004. 10.1136/bjo.2003.017731 0007-1161 WOS:000188302800032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
British Journal of Ophthalmology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
280-285 |
dc.publisher.none.fl_str_mv |
B M J Publishing Group |
publisher.none.fl_str_mv |
B M J Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268367147630592 |