Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes

Detalhes bibliográficos
Autor(a) principal: Gomes, José Álvaro Pereira [UNIFESP]
Data de Publicação: 2004
Outros Autores: Dua, H. S., Rizzo, Luiz V., Nishi, Mauro [UNIFESP], Joseph, A., Donoso, L. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1136/bjo.2003.017731
http://repositorio.unifesp.br/handle/11600/27599
Resumo: Background/aims: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. the purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.Methods: Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.Results: Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).Conclusion: Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes.
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spelling Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytesBackground/aims: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. the purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.Methods: Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.Results: Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).Conclusion: Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes.Wills Eye Hosp & Res Inst, Div Res, Philadelphia, PA 19107 USAUniversidade Federal de São Paulo, UNIFESP, São Paulo, BrazilUniv Nottingham, Dept Ophthalmol, Nottingham NG7 2RD, EnglandNIH, Bethesda, MD 20892 USAUniv São Paulo, BR-05508 São Paulo, BrazilFund EJ Zerbini, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, São Paulo, BrazilWeb of ScienceB M J Publishing GroupWills Eye Hosp & Res InstUniversidade Federal de São Paulo (UNIFESP)Univ NottinghamNIHUniversidade de São Paulo (USP)Fund EJ ZerbiniGomes, José Álvaro Pereira [UNIFESP]Dua, H. S.Rizzo, Luiz V.Nishi, Mauro [UNIFESP]Joseph, A.Donoso, L. A.2016-01-24T12:34:16Z2016-01-24T12:34:16Z2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion280-285http://dx.doi.org/10.1136/bjo.2003.017731British Journal of Ophthalmology. London: B M J Publishing Group, v. 88, n. 2, p. 280-285, 2004.10.1136/bjo.2003.0177310007-1161http://repositorio.unifesp.br/handle/11600/27599WOS:000188302800032engBritish Journal of Ophthalmologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-26T10:22:12Zoai:repositorio.unifesp.br/:11600/27599Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-26T10:22:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
title Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
spellingShingle Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
Gomes, José Álvaro Pereira [UNIFESP]
title_short Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
title_full Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
title_fullStr Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
title_full_unstemmed Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
title_sort Ocular surface epithelium induces expression of human mucosal lymphocyte antigen (HML-1) on peripheral blood lymphocytes
author Gomes, José Álvaro Pereira [UNIFESP]
author_facet Gomes, José Álvaro Pereira [UNIFESP]
Dua, H. S.
Rizzo, Luiz V.
Nishi, Mauro [UNIFESP]
Joseph, A.
Donoso, L. A.
author_role author
author2 Dua, H. S.
Rizzo, Luiz V.
Nishi, Mauro [UNIFESP]
Joseph, A.
Donoso, L. A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Wills Eye Hosp & Res Inst
Universidade Federal de São Paulo (UNIFESP)
Univ Nottingham
NIH
Universidade de São Paulo (USP)
Fund EJ Zerbini
dc.contributor.author.fl_str_mv Gomes, José Álvaro Pereira [UNIFESP]
Dua, H. S.
Rizzo, Luiz V.
Nishi, Mauro [UNIFESP]
Joseph, A.
Donoso, L. A.
description Background/aims: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. the purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.Methods: Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.Results: Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).Conclusion: Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
2016-01-24T12:34:16Z
2016-01-24T12:34:16Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1136/bjo.2003.017731
British Journal of Ophthalmology. London: B M J Publishing Group, v. 88, n. 2, p. 280-285, 2004.
10.1136/bjo.2003.017731
0007-1161
http://repositorio.unifesp.br/handle/11600/27599
WOS:000188302800032
url http://dx.doi.org/10.1136/bjo.2003.017731
http://repositorio.unifesp.br/handle/11600/27599
identifier_str_mv British Journal of Ophthalmology. London: B M J Publishing Group, v. 88, n. 2, p. 280-285, 2004.
10.1136/bjo.2003.017731
0007-1161
WOS:000188302800032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv British Journal of Ophthalmology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 280-285
dc.publisher.none.fl_str_mv B M J Publishing Group
publisher.none.fl_str_mv B M J Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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