Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/26200 http://dx.doi.org/10.1046/j.1365-3024.2000.00260.x |
Resumo: | Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). the present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronically infected chagasic individuals. the cellular immune response was measured by in-vitro T-cell proliferation and by interferon (INF)-gamma, interleukin (IL)-4 and IL-10 production in response to a whole-parasite homogenate and the recombinant protein. the peripheral blood mononuclear cells of 78.6% of 28 chagasic patients responded to the recombinant protein as estimated by T-cell proliferation. With respect to cytokine production, 88% of the cells of the chagasic individuals produced IFN-gamma on stimulation with the recombinant protein. in contrast, IL-4 or IL-10 were minimally produced in response to TS. the cellular immune response was specific because most healthy individuals never exposed to T. cruzi failed to react with this recombinant protein. the plasma of 71.4% or 100% of chagasic patients had Ige antibodies as determined by ELISA or by the presence of TS inhibitory antibodies, respectively. We conclude that the catalytic domain of TS is recognized by IFN-gamma producing type I cells and antibodies in a large proportion of patients infected with T. cruzi. |
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Ribeirao, Marcelo [UNIFESP]Pereira-Chioccola, Vera Lucia [UNIFESP]Renia, L.Fragata, A. A.Schenkman, Sergio [UNIFESP]Rodrigues, Mauricio Martins [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Pazzanese Cardiol Estado São PauloUniv Paris 052016-01-24T12:30:57Z2016-01-24T12:30:57Z2000-01-01Parasite Immunology. Oxford: Blackwell Publishing Ltd, v. 22, n. 1, p. 49-53, 2000.0141-9838http://repositorio.unifesp.br/handle/11600/26200http://dx.doi.org/10.1046/j.1365-3024.2000.00260.x10.1046/j.1365-3024.2000.00260.xWOS:000085737500007Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). the present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronically infected chagasic individuals. the cellular immune response was measured by in-vitro T-cell proliferation and by interferon (INF)-gamma, interleukin (IL)-4 and IL-10 production in response to a whole-parasite homogenate and the recombinant protein. the peripheral blood mononuclear cells of 78.6% of 28 chagasic patients responded to the recombinant protein as estimated by T-cell proliferation. With respect to cytokine production, 88% of the cells of the chagasic individuals produced IFN-gamma on stimulation with the recombinant protein. in contrast, IL-4 or IL-10 were minimally produced in response to TS. the cellular immune response was specific because most healthy individuals never exposed to T. cruzi failed to react with this recombinant protein. the plasma of 71.4% or 100% of chagasic patients had Ige antibodies as determined by ELISA or by the presence of TS inhibitory antibodies, respectively. We conclude that the catalytic domain of TS is recognized by IFN-gamma producing type I cells and antibodies in a large proportion of patients infected with T. cruzi.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilPazzanese Cardiol Estado São Paulo, Inst Dante, Lab Xenodiagnost, São Paulo, BrazilUniv Paris 05, Hop Cochin, Lab Immunol Pathol Infect & Tumorales, INSERM,U445,Inst Cochin Genet Mol, F-75014 Paris, FranceUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Science49-53engBlackwell Publishing LtdParasite ImmunologyTrypanosomessialidasescell-mediated immune responseChagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/262002022-11-04 15:08:27.288metadata only accessoai:repositorio.unifesp.br:11600/26200Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T18:08:27Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
title |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
spellingShingle |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase Ribeirao, Marcelo [UNIFESP] Trypanosomes sialidases cell-mediated immune response |
title_short |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
title_full |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
title_fullStr |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
title_full_unstemmed |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
title_sort |
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase |
author |
Ribeirao, Marcelo [UNIFESP] |
author_facet |
Ribeirao, Marcelo [UNIFESP] Pereira-Chioccola, Vera Lucia [UNIFESP] Renia, L. Fragata, A. A. Schenkman, Sergio [UNIFESP] Rodrigues, Mauricio Martins [UNIFESP] |
author_role |
author |
author2 |
Pereira-Chioccola, Vera Lucia [UNIFESP] Renia, L. Fragata, A. A. Schenkman, Sergio [UNIFESP] Rodrigues, Mauricio Martins [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Pazzanese Cardiol Estado São Paulo Univ Paris 05 |
dc.contributor.author.fl_str_mv |
Ribeirao, Marcelo [UNIFESP] Pereira-Chioccola, Vera Lucia [UNIFESP] Renia, L. Fragata, A. A. Schenkman, Sergio [UNIFESP] Rodrigues, Mauricio Martins [UNIFESP] |
dc.subject.eng.fl_str_mv |
Trypanosomes sialidases cell-mediated immune response |
topic |
Trypanosomes sialidases cell-mediated immune response |
description |
Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). the present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronically infected chagasic individuals. the cellular immune response was measured by in-vitro T-cell proliferation and by interferon (INF)-gamma, interleukin (IL)-4 and IL-10 production in response to a whole-parasite homogenate and the recombinant protein. the peripheral blood mononuclear cells of 78.6% of 28 chagasic patients responded to the recombinant protein as estimated by T-cell proliferation. With respect to cytokine production, 88% of the cells of the chagasic individuals produced IFN-gamma on stimulation with the recombinant protein. in contrast, IL-4 or IL-10 were minimally produced in response to TS. the cellular immune response was specific because most healthy individuals never exposed to T. cruzi failed to react with this recombinant protein. the plasma of 71.4% or 100% of chagasic patients had Ige antibodies as determined by ELISA or by the presence of TS inhibitory antibodies, respectively. We conclude that the catalytic domain of TS is recognized by IFN-gamma producing type I cells and antibodies in a large proportion of patients infected with T. cruzi. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:30:57Z |
dc.date.available.fl_str_mv |
2016-01-24T12:30:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Parasite Immunology. Oxford: Blackwell Publishing Ltd, v. 22, n. 1, p. 49-53, 2000. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/26200 http://dx.doi.org/10.1046/j.1365-3024.2000.00260.x |
dc.identifier.issn.none.fl_str_mv |
0141-9838 |
dc.identifier.doi.none.fl_str_mv |
10.1046/j.1365-3024.2000.00260.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000085737500007 |
identifier_str_mv |
Parasite Immunology. Oxford: Blackwell Publishing Ltd, v. 22, n. 1, p. 49-53, 2000. 0141-9838 10.1046/j.1365-3024.2000.00260.x WOS:000085737500007 |
url |
http://repositorio.unifesp.br/handle/11600/26200 http://dx.doi.org/10.1046/j.1365-3024.2000.00260.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Parasite Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
49-53 |
dc.publisher.none.fl_str_mv |
Blackwell Publishing Ltd |
publisher.none.fl_str_mv |
Blackwell Publishing Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764183594336256 |