Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein

Detalhes bibliográficos
Autor(a) principal: Pereira, Catia Mendes [UNIFESP]
Data de Publicação: 1998
Outros Autores: Yamauchi, Lucy M., Levin, Mariano J., Silveira, José Franco da, Castilho, Beatriz A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13077.x
http://repositorio.unifesp.br/handle/11600/25920
Resumo: The JL8 protein antigen from Trypanosoma cii , a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the LamB protein of Escherichia coli as a carl icr of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the LamB protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. the fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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spelling Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB proteinTrypanosoma cruziimmunodominant repetitive antigenB cell epitope mappingEscherichia coli LamB proteinChagas diseaseThe JL8 protein antigen from Trypanosoma cii , a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the LamB protein of Escherichia coli as a carl icr of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the LamB protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. the fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilIngebi, RA-1428 Buenos Aires, DF, ArgentinaUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)IngebiPereira, Catia Mendes [UNIFESP]Yamauchi, Lucy M.Levin, Mariano J.Silveira, José Franco daCastilho, Beatriz A.2016-01-24T12:30:37Z2016-01-24T12:30:37Z1998-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion125-131http://dx.doi.org/10.1111/j.1574-6968.1998.tb13077.xFems Microbiology Letters. Amsterdam: Elsevier B.V., v. 164, n. 1, p. 125-131, 1998.10.1111/j.1574-6968.1998.tb13077.x0378-1097http://repositorio.unifesp.br/handle/11600/25920WOS:000074571400019engFems Microbiology Lettersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-06-02T09:02:07Zoai:repositorio.unifesp.br/:11600/25920Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-06-02T09:02:07Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
title Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
spellingShingle Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
Pereira, Catia Mendes [UNIFESP]
Trypanosoma cruzi
immunodominant repetitive antigen
B cell epitope mapping
Escherichia coli LamB protein
Chagas disease
title_short Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
title_full Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
title_fullStr Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
title_full_unstemmed Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
title_sort Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
author Pereira, Catia Mendes [UNIFESP]
author_facet Pereira, Catia Mendes [UNIFESP]
Yamauchi, Lucy M.
Levin, Mariano J.
Silveira, José Franco da
Castilho, Beatriz A.
author_role author
author2 Yamauchi, Lucy M.
Levin, Mariano J.
Silveira, José Franco da
Castilho, Beatriz A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Ingebi
dc.contributor.author.fl_str_mv Pereira, Catia Mendes [UNIFESP]
Yamauchi, Lucy M.
Levin, Mariano J.
Silveira, José Franco da
Castilho, Beatriz A.
dc.subject.por.fl_str_mv Trypanosoma cruzi
immunodominant repetitive antigen
B cell epitope mapping
Escherichia coli LamB protein
Chagas disease
topic Trypanosoma cruzi
immunodominant repetitive antigen
B cell epitope mapping
Escherichia coli LamB protein
Chagas disease
description The JL8 protein antigen from Trypanosoma cii , a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the LamB protein of Escherichia coli as a carl icr of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the LamB protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. the fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
publishDate 1998
dc.date.none.fl_str_mv 1998-07-01
2016-01-24T12:30:37Z
2016-01-24T12:30:37Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1574-6968.1998.tb13077.x
Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 164, n. 1, p. 125-131, 1998.
10.1111/j.1574-6968.1998.tb13077.x
0378-1097
http://repositorio.unifesp.br/handle/11600/25920
WOS:000074571400019
url http://dx.doi.org/10.1111/j.1574-6968.1998.tb13077.x
http://repositorio.unifesp.br/handle/11600/25920
identifier_str_mv Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 164, n. 1, p. 125-131, 1998.
10.1111/j.1574-6968.1998.tb13077.x
0378-1097
WOS:000074571400019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fems Microbiology Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 125-131
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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