Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein

Detalhes bibliográficos
Autor(a) principal: Pereira, Catia Mendes [UNIFESP]
Data de Publicação: 2004
Outros Autores: Franco da Silveira, José [UNIFESP], Duranti, M., Cunha-Neto, E., Zingales, B., Castilho, Beatriz Amaral de [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000tdd5
Texto Completo: http://dx.doi.org/10.1016/j.femsle.2004.04.040
http://repositorio.unifesp.br/handle/11600/27792
Resumo: B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.
id UFSP_3d053b708d76965d6a49f086cf44227a
oai_identifier_str oai:repositorio.unifesp.br/:11600/27792
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB proteinTrypanosoma cruziimmunodominant repetitive antigenB13 antigenB cell epitope mappingEscherichia coli LamB proteinB13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.UNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-01023062 São Paulo, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, BrazilUniv São Paulo, Fac Med, Inst Coracao, Lab Immunol Transplantes, BR-01498 São Paulo, BrazilUNIFESP, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-01023062 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Pereira, Catia Mendes [UNIFESP]Franco da Silveira, José [UNIFESP]Duranti, M.Cunha-Neto, E.Zingales, B.Castilho, Beatriz Amaral de [UNIFESP]2016-01-24T12:37:12Z2016-01-24T12:37:12Z2004-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion237-242http://dx.doi.org/10.1016/j.femsle.2004.04.040Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 235, n. 2, p. 237-242, 2004.10.1016/j.femsle.2004.04.0400378-1097http://repositorio.unifesp.br/handle/11600/27792WOS:000222144400004ark:/48912/001300000tdd5engFems Microbiology Lettersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-08T11:55:01Zoai:repositorio.unifesp.br/:11600/27792Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:37:17.507870Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
title Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
spellingShingle Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
Pereira, Catia Mendes [UNIFESP]
Trypanosoma cruzi
immunodominant repetitive antigen
B13 antigen
B cell epitope mapping
Escherichia coli LamB protein
title_short Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
title_full Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
title_fullStr Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
title_full_unstemmed Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
title_sort Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein
author Pereira, Catia Mendes [UNIFESP]
author_facet Pereira, Catia Mendes [UNIFESP]
Franco da Silveira, José [UNIFESP]
Duranti, M.
Cunha-Neto, E.
Zingales, B.
Castilho, Beatriz Amaral de [UNIFESP]
author_role author
author2 Franco da Silveira, José [UNIFESP]
Duranti, M.
Cunha-Neto, E.
Zingales, B.
Castilho, Beatriz Amaral de [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Pereira, Catia Mendes [UNIFESP]
Franco da Silveira, José [UNIFESP]
Duranti, M.
Cunha-Neto, E.
Zingales, B.
Castilho, Beatriz Amaral de [UNIFESP]
dc.subject.por.fl_str_mv Trypanosoma cruzi
immunodominant repetitive antigen
B13 antigen
B cell epitope mapping
Escherichia coli LamB protein
topic Trypanosoma cruzi
immunodominant repetitive antigen
B13 antigen
B cell epitope mapping
Escherichia coli LamB protein
description B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-15
2016-01-24T12:37:12Z
2016-01-24T12:37:12Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.femsle.2004.04.040
Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 235, n. 2, p. 237-242, 2004.
10.1016/j.femsle.2004.04.040
0378-1097
http://repositorio.unifesp.br/handle/11600/27792
WOS:000222144400004
dc.identifier.dark.fl_str_mv ark:/48912/001300000tdd5
url http://dx.doi.org/10.1016/j.femsle.2004.04.040
http://repositorio.unifesp.br/handle/11600/27792
identifier_str_mv Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 235, n. 2, p. 237-242, 2004.
10.1016/j.femsle.2004.04.040
0378-1097
WOS:000222144400004
ark:/48912/001300000tdd5
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fems Microbiology Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 237-242
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1818602517069561856

Registros relacionados