Screening do alelo HLA-B*5701 em associação a hipersensibilidade ao abacavir em pacientes HIV positivos do estado de Mato Grosso

• Autor(a) principal: Araújo, Claudinéia de [UNIFESP]
• Data de Publicação: 2010
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/10068
• topic: Abacavir; Alelo HLA-B*57:01; Hipersensibilidade; HIV; Alelos; Reação em cadeia da polimerase; Abacavir; HLA-B*57:01; HIV patients; HIV; Alleles; Hypersensitivity; Polymerase chain reaction

Hypersensitivity reaction to abacavir is strongly associated with the presence of the HLA-B*57:01 allele. This study was designed to establish the prevalence of HLA-B*57:01 among HIV-1 infected individuals in Brazil. A total of 517 consecutive outpatient’s clinics of the State Reference Center in High and Medium Complexity - CERMAC and forwarded to other medical care unit of the Mato Grosso State were followed in this study from february 2008 through july 2010. The presence of HLA-B*57:01 was determined by Nested-PCR with HLA-B*57 and HLA-B*57:01 sequence-specific primers (PCR-SSP). A total of 517 patients were enrolled and randomly assigned to a study group. Of these, 385 (74.5%) were negative for HLA-B*57; 103 (19.9%) were positive for HLA-B*57 and 29 (5.6%) were positive for HLA-B*57:01. Of the HLA-B*57 and HLA-B*57:01 negative patients, the median age was 40 years; 205 (53.2%) were male sex and 242 (62.9%) were Caucasians. Among the patients positive for allele HLA-B*57, the mean age were 38 years; 47 (45.6%) were male sex and 64 (62.1%) were Caucasians. Of the patients positive for allele HLA-B*57:01, the median age was 40 years; 17 (58.6%) were men and 15 (57.7%) were caucasian. An abacavir containing regimen was administerede to 68 patients during the study period, the HLA-B*57:01 alelle was found in 7 (10.3%) of these and the in Hypersensitivity reaction was clinically diagnosed in 4 of these patients, with a statistically significant incidence (p<0.001).