Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/36745 http://dx.doi.org/10.1186/1471-2202-14-97 |
Resumo: | Background: Bone marrow is an accessible source of progenitor cells, which have been investigated as treatment for neurological diseases in a number of clinical trials. Here we evaluated the potential benefit of bone marrow cells in protecting against convulsive seizures induced by maximum electroconvulsive shock (MES), a widely used model for screening of anti-epileptic drugs. Behavioral and inflammatory responses were measured after MES induction in order to verify the effects promoted by transplantation of bone marrow cells. To assess the anticonvulsant effects of bone marrow cell transplantation, we measured the frequency and duration of tonic seizure, the mortality rate, the microglial expression and the blood levels of cytokine IL-1, IL-6, IL-10 and TNF-alpha after MES induction. We hypothesized that these behavioral and inflammatory responses to a strong stimulus such as a convulsive seizure could be modified by the transplantation of bone marrow cells.Results: Bone marrow transplanted cells altered the convulsive threshold and showed anticonvulsant effect by protecting from tonic seizures. Bone marrow cells modified the microglial expression in the analyzed brain areas, increased the IL-10 and attenuate IL-6 levels.Conclusions: Bone marrow cells exert protective effects by blocking the course of electroconvulsive seizures. Additionally, electroconvulsive seizures induced acute inflammatory responses by altering the pattern of microglia expression, as well as in IL-6 and IL-10 levels. Our findings also indicated that the anticonvulsant effects of these cells can be tested with the MES model following the same paradigm used for drug testing in pharmacological screening. Studies on the inflammatory reaction in response to acute seizures in the presence of transplanted bone marrow cells might open a wide range of discussions on the mechanisms relevant to the pathophysiology of epilepsies. |
id |
UFSP_068b668d8d6d92194cd306a5a9531593 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/36745 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Ferrazoli, Eneas Galdini [UNIFESP]Blanco, Miriam Marcela [UNIFESP]Bittencourt, Simone [UNIFESP]Bachi, Andre Luis Lacerda [UNIFESP]Bahia, LucianaSoares, Milena Botelho PereiraRibeiro-dos-Santos, RicardoMello, Luiz Eugenio [UNIFESP]Longo, Beatriz Monteiro [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Fundacao Oswaldo CruzHosp Sao Rafael2016-01-24T14:34:24Z2016-01-24T14:34:24Z2013-09-06Bmc Neuroscience. London: Biomed Central Ltd, v. 14, 8 p., 2013.1471-2202http://repositorio.unifesp.br/handle/11600/36745http://dx.doi.org/10.1186/1471-2202-14-97WOS000324241800001.pdf10.1186/1471-2202-14-97WOS:000324241800001Background: Bone marrow is an accessible source of progenitor cells, which have been investigated as treatment for neurological diseases in a number of clinical trials. Here we evaluated the potential benefit of bone marrow cells in protecting against convulsive seizures induced by maximum electroconvulsive shock (MES), a widely used model for screening of anti-epileptic drugs. Behavioral and inflammatory responses were measured after MES induction in order to verify the effects promoted by transplantation of bone marrow cells. To assess the anticonvulsant effects of bone marrow cell transplantation, we measured the frequency and duration of tonic seizure, the mortality rate, the microglial expression and the blood levels of cytokine IL-1, IL-6, IL-10 and TNF-alpha after MES induction. We hypothesized that these behavioral and inflammatory responses to a strong stimulus such as a convulsive seizure could be modified by the transplantation of bone marrow cells.Results: Bone marrow transplanted cells altered the convulsive threshold and showed anticonvulsant effect by protecting from tonic seizures. Bone marrow cells modified the microglial expression in the analyzed brain areas, increased the IL-10 and attenuate IL-6 levels.Conclusions: Bone marrow cells exert protective effects by blocking the course of electroconvulsive seizures. Additionally, electroconvulsive seizures induced acute inflammatory responses by altering the pattern of microglia expression, as well as in IL-6 and IL-10 levels. Our findings also indicated that the anticonvulsant effects of these cells can be tested with the MES model following the same paradigm used for drug testing in pharmacological screening. Studies on the inflammatory reaction in response to acute seizures in the presence of transplanted bone marrow cells might open a wide range of discussions on the mechanisms relevant to the pathophysiology of epilepsies.Associacao Beneficente de Coleta de Sangue da Fundacao de Apoio a Pesquisa- UNIFESP (FAP-Colsan)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, UNIFESP, Dept Fisiol, Lab Neurofisiol, BR-04023066 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Imunol, Dept Microimunoparasitol, São Paulo, BrazilFundacao Oswaldo Cruz, Ctr Pesquisas Goncalo Moniz, Salvador, BA, BrazilHosp Sao Rafael, Ctr Biotecnol & Terapia Celular, Salvador, BA, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Fisiol, Lab Neurofisiol, BR-04023066 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Imunol, Dept Microimunoparasitol, São Paulo, BrazilWeb of Science8engBiomed Central LtdBmc NeuroscienceBone marrowCell transplantationElectroconvulsive shockTonic seizureMicrogliaHippocampusAnticonvulsant activity of bone marrow cells in electroconvulsive seizures in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000324241800001.pdfapplication/pdf583379${dspace.ui.url}/bitstream/11600/36745/1/WOS000324241800001.pdfcc62d3b5cefbf1c6f55123a80ff5b5c0MD51open accessTEXTWOS000324241800001.pdf.txtWOS000324241800001.pdf.txtExtracted texttext/plain40420${dspace.ui.url}/bitstream/11600/36745/2/WOS000324241800001.pdf.txt3414a98671e7013aeecdbc1bd4352bbfMD52open access11600/367452021-09-30 17:26:15.379open accessoai:repositorio.unifesp.br:11600/36745Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:10:47.523242Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
title |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
spellingShingle |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice Ferrazoli, Eneas Galdini [UNIFESP] Bone marrow Cell transplantation Electroconvulsive shock Tonic seizure Microglia Hippocampus |
title_short |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
title_full |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
title_fullStr |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
title_full_unstemmed |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
title_sort |
Anticonvulsant activity of bone marrow cells in electroconvulsive seizures in mice |
author |
Ferrazoli, Eneas Galdini [UNIFESP] |
author_facet |
Ferrazoli, Eneas Galdini [UNIFESP] Blanco, Miriam Marcela [UNIFESP] Bittencourt, Simone [UNIFESP] Bachi, Andre Luis Lacerda [UNIFESP] Bahia, Luciana Soares, Milena Botelho Pereira Ribeiro-dos-Santos, Ricardo Mello, Luiz Eugenio [UNIFESP] Longo, Beatriz Monteiro [UNIFESP] |
author_role |
author |
author2 |
Blanco, Miriam Marcela [UNIFESP] Bittencourt, Simone [UNIFESP] Bachi, Andre Luis Lacerda [UNIFESP] Bahia, Luciana Soares, Milena Botelho Pereira Ribeiro-dos-Santos, Ricardo Mello, Luiz Eugenio [UNIFESP] Longo, Beatriz Monteiro [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fundacao Oswaldo Cruz Hosp Sao Rafael |
dc.contributor.author.fl_str_mv |
Ferrazoli, Eneas Galdini [UNIFESP] Blanco, Miriam Marcela [UNIFESP] Bittencourt, Simone [UNIFESP] Bachi, Andre Luis Lacerda [UNIFESP] Bahia, Luciana Soares, Milena Botelho Pereira Ribeiro-dos-Santos, Ricardo Mello, Luiz Eugenio [UNIFESP] Longo, Beatriz Monteiro [UNIFESP] |
dc.subject.eng.fl_str_mv |
Bone marrow Cell transplantation Electroconvulsive shock Tonic seizure Microglia Hippocampus |
topic |
Bone marrow Cell transplantation Electroconvulsive shock Tonic seizure Microglia Hippocampus |
description |
Background: Bone marrow is an accessible source of progenitor cells, which have been investigated as treatment for neurological diseases in a number of clinical trials. Here we evaluated the potential benefit of bone marrow cells in protecting against convulsive seizures induced by maximum electroconvulsive shock (MES), a widely used model for screening of anti-epileptic drugs. Behavioral and inflammatory responses were measured after MES induction in order to verify the effects promoted by transplantation of bone marrow cells. To assess the anticonvulsant effects of bone marrow cell transplantation, we measured the frequency and duration of tonic seizure, the mortality rate, the microglial expression and the blood levels of cytokine IL-1, IL-6, IL-10 and TNF-alpha after MES induction. We hypothesized that these behavioral and inflammatory responses to a strong stimulus such as a convulsive seizure could be modified by the transplantation of bone marrow cells.Results: Bone marrow transplanted cells altered the convulsive threshold and showed anticonvulsant effect by protecting from tonic seizures. Bone marrow cells modified the microglial expression in the analyzed brain areas, increased the IL-10 and attenuate IL-6 levels.Conclusions: Bone marrow cells exert protective effects by blocking the course of electroconvulsive seizures. Additionally, electroconvulsive seizures induced acute inflammatory responses by altering the pattern of microglia expression, as well as in IL-6 and IL-10 levels. Our findings also indicated that the anticonvulsant effects of these cells can be tested with the MES model following the same paradigm used for drug testing in pharmacological screening. Studies on the inflammatory reaction in response to acute seizures in the presence of transplanted bone marrow cells might open a wide range of discussions on the mechanisms relevant to the pathophysiology of epilepsies. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-09-06 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:34:24Z |
dc.date.available.fl_str_mv |
2016-01-24T14:34:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Bmc Neuroscience. London: Biomed Central Ltd, v. 14, 8 p., 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/36745 http://dx.doi.org/10.1186/1471-2202-14-97 |
dc.identifier.issn.none.fl_str_mv |
1471-2202 |
dc.identifier.file.none.fl_str_mv |
WOS000324241800001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2202-14-97 |
dc.identifier.wos.none.fl_str_mv |
WOS:000324241800001 |
identifier_str_mv |
Bmc Neuroscience. London: Biomed Central Ltd, v. 14, 8 p., 2013. 1471-2202 WOS000324241800001.pdf 10.1186/1471-2202-14-97 WOS:000324241800001 |
url |
http://repositorio.unifesp.br/handle/11600/36745 http://dx.doi.org/10.1186/1471-2202-14-97 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Bmc Neuroscience |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/36745/1/WOS000324241800001.pdf ${dspace.ui.url}/bitstream/11600/36745/2/WOS000324241800001.pdf.txt |
bitstream.checksum.fl_str_mv |
cc62d3b5cefbf1c6f55123a80ff5b5c0 3414a98671e7013aeecdbc1bd4352bbf |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460259448225792 |