Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies

Detalhes bibliográficos
Autor(a) principal: Verbisck, Newton Valério [UNIFESP]
Data de Publicação: 1998
Outros Autores: Da-Silva, S. [UNIFESP], Mortara, Renato Arruda [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/706
http://dx.doi.org/10.1590/S0100-879X1998001200011
Resumo: We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components.
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spelling Verbisck, Newton Valério [UNIFESP]Da-Silva, S. [UNIFESP]Mortara, Renato Arruda [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:24:48Z2015-06-14T13:24:48Z1998-12-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998.0100-879Xhttp://repositorio.unifesp.br/handle/11600/706http://dx.doi.org/10.1590/S0100-879X1998001200011S0100-879X1998001200011.pdfS0100-879X199800120001110.1590/S0100-879X1998001200011WOS:000077232400011We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components.Universidade Federal de São Paulo (UNIFESP)UNIFESP, EPMSciELO1583-1591engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchTrypanosoma cruziamastigotemonoclonal antibodiessurface antigenpolymorphismTrypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X1998001200011.pdfapplication/pdf294397${dspace.ui.url}/bitstream/11600/706/1/S0100-879X1998001200011.pdf64120f6e4e3955d53481a3fec2fd2864MD51open accessTEXTS0100-879X1998001200011.pdf.txtS0100-879X1998001200011.pdf.txtExtracted texttext/plain33024${dspace.ui.url}/bitstream/11600/706/2/S0100-879X1998001200011.pdf.txt93330fca99e8201e0fecf109eabb529bMD52open access11600/7062022-06-02 09:05:53.865open accessoai:repositorio.unifesp.br:11600/706Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:13:42.720821Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
title Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
spellingShingle Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
Verbisck, Newton Valério [UNIFESP]
Trypanosoma cruzi
amastigote
monoclonal antibodies
surface antigen
polymorphism
title_short Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
title_full Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
title_fullStr Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
title_full_unstemmed Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
title_sort Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
author Verbisck, Newton Valério [UNIFESP]
author_facet Verbisck, Newton Valério [UNIFESP]
Da-Silva, S. [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
author_role author
author2 Da-Silva, S. [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
author2_role author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Verbisck, Newton Valério [UNIFESP]
Da-Silva, S. [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
dc.subject.eng.fl_str_mv Trypanosoma cruzi
amastigote
monoclonal antibodies
surface antigen
polymorphism
topic Trypanosoma cruzi
amastigote
monoclonal antibodies
surface antigen
polymorphism
description We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components.
publishDate 1998
dc.date.issued.fl_str_mv 1998-12-01
dc.date.accessioned.fl_str_mv 2015-06-14T13:24:48Z
dc.date.available.fl_str_mv 2015-06-14T13:24:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/706
http://dx.doi.org/10.1590/S0100-879X1998001200011
dc.identifier.issn.none.fl_str_mv 0100-879X
dc.identifier.file.none.fl_str_mv S0100-879X1998001200011.pdf
dc.identifier.scielo.none.fl_str_mv S0100-879X1998001200011
dc.identifier.doi.none.fl_str_mv 10.1590/S0100-879X1998001200011
dc.identifier.wos.none.fl_str_mv WOS:000077232400011
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998.
0100-879X
S0100-879X1998001200011.pdf
S0100-879X1998001200011
10.1590/S0100-879X1998001200011
WOS:000077232400011
url http://repositorio.unifesp.br/handle/11600/706
http://dx.doi.org/10.1590/S0100-879X1998001200011
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
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