Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/706 http://dx.doi.org/10.1590/S0100-879X1998001200011 |
Resumo: | We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components. |
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Verbisck, Newton Valério [UNIFESP]Da-Silva, S. [UNIFESP]Mortara, Renato Arruda [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:24:48Z2015-06-14T13:24:48Z1998-12-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998.0100-879Xhttp://repositorio.unifesp.br/handle/11600/706http://dx.doi.org/10.1590/S0100-879X1998001200011S0100-879X1998001200011.pdfS0100-879X199800120001110.1590/S0100-879X1998001200011WOS:000077232400011We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components.Universidade Federal de São Paulo (UNIFESP)UNIFESP, EPMSciELO1583-1591engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchTrypanosoma cruziamastigotemonoclonal antibodiessurface antigenpolymorphismTrypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X1998001200011.pdfapplication/pdf294397${dspace.ui.url}/bitstream/11600/706/1/S0100-879X1998001200011.pdf64120f6e4e3955d53481a3fec2fd2864MD51open accessTEXTS0100-879X1998001200011.pdf.txtS0100-879X1998001200011.pdf.txtExtracted texttext/plain33024${dspace.ui.url}/bitstream/11600/706/2/S0100-879X1998001200011.pdf.txt93330fca99e8201e0fecf109eabb529bMD52open access11600/7062022-06-02 09:05:53.865open accessoai:repositorio.unifesp.br:11600/706Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:05:53Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
title |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
spellingShingle |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies Verbisck, Newton Valério [UNIFESP] Trypanosoma cruzi amastigote monoclonal antibodies surface antigen polymorphism |
title_short |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
title_full |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
title_fullStr |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
title_full_unstemmed |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
title_sort |
Trypanosoma cruzi: amastigote polymorphism defined by monoclonal antibodies |
author |
Verbisck, Newton Valério [UNIFESP] |
author_facet |
Verbisck, Newton Valério [UNIFESP] Da-Silva, S. [UNIFESP] Mortara, Renato Arruda [UNIFESP] |
author_role |
author |
author2 |
Da-Silva, S. [UNIFESP] Mortara, Renato Arruda [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Verbisck, Newton Valério [UNIFESP] Da-Silva, S. [UNIFESP] Mortara, Renato Arruda [UNIFESP] |
dc.subject.eng.fl_str_mv |
Trypanosoma cruzi amastigote monoclonal antibodies surface antigen polymorphism |
topic |
Trypanosoma cruzi amastigote monoclonal antibodies surface antigen polymorphism |
description |
We have raised monoclonal antibodies (mAbs) directed towards amastigote forms of Trypanosoma cruzi, and shown that mAbs 1D9 and 4B9 are carbohydrate while mAb 4B5 activity is resistant to periodate oxidation of the antigen. Here we used an ELISA to quantitate and compare the expression of surface epitopes on fixed parasites among different parasite isolates. The expression of markers varied among T. cruzi amastigotes isolated from infected cells or after extracellular differentiation of trypomastigotes. Moreover, we also observed an extensive polymorphic expression of these epitopes among amastigotes derived from different strains and clones. For instance, mAb 2C2 strongly and evenly reacted with 9 strains and clones (G, Y, CL, Tulahuen, MD, and F, and clones Sylvio X-10/4, D11, and CL.B), with absorbance at 492 nm (A492 nm) from 0.6 to 0.8. By contrast, mAb 4B5 had a higher expression in Tulahuen amastigotes (around 0.9 at 492 nm) whereas its reactivity with amastigotes from clones CL.B, Sylvio X-10/4 and D11 was much lower (around 0.4). mAb 1D9 displayed an interesting pattern of reactivity with amastigotes of the different strains and clones (A492 nm of G>D11³Sylvio X-10/4 = MD>Tulahuen = F = Y>CL>CL.B). Finally, we observed that mAb 4B9 had the lowest reaction with the parasites studied, with higher values of A492 nm with Y strain (around 0.6) and lower values with Tulahuen, F and CL.B strains (around 0.2). Immunoblotting analysis also showed extensive variations among amastigotes of the various parasite isolates and mAbs 4B9, 1D9 and 4B5 revealed significant differences in expression between clones and parental strains. These data describe a previously uncharacterized polymorphism of T. cruzi amastigote surface components. |
publishDate |
1998 |
dc.date.issued.fl_str_mv |
1998-12-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:24:48Z |
dc.date.available.fl_str_mv |
2015-06-14T13:24:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/706 http://dx.doi.org/10.1590/S0100-879X1998001200011 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X1998001200011.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X1998001200011 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X1998001200011 |
dc.identifier.wos.none.fl_str_mv |
WOS:000077232400011 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 12, p. 1583-1591, 1998. 0100-879X S0100-879X1998001200011.pdf S0100-879X1998001200011 10.1590/S0100-879X1998001200011 WOS:000077232400011 |
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http://repositorio.unifesp.br/handle/11600/706 http://dx.doi.org/10.1590/S0100-879X1998001200011 |
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eng |
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Brazilian Journal of Medical and Biological Research |
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Associação Brasileira de Divulgação Científica |
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