Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1111/and.12959 https://repositorio.unifesp.br/handle/11600/55602 |
Resumo: | The aim of this study was to evaluate the antioxidant effect of in vitro supplementation with vitamin E in human spermatozoon incubated with an oxidative stress inducer. In this study, semen samples from 30 patients were collected and with one aliquot we performed semen analysis according to WHO. The remaining volume was divided into four aliquots: group C: incubated with BWW medium |
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3465 |
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Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stressantioxidantmale infertilityoxidative stressreactive oxygen speciesvitamin EThe aim of this study was to evaluate the antioxidant effect of in vitro supplementation with vitamin E in human spermatozoon incubated with an oxidative stress inducer. In this study, semen samples from 30 patients were collected and with one aliquot we performed semen analysis according to WHO. The remaining volume was divided into four aliquots: group C: incubated with BWW mediumgroup I: incubated with 5mmol 1-1 hydrogen peroxidegroup A: incubated with 40mol 1-1 vitamin Eand group AI: incubated with both them. After incubations, sperm functional analyses were performed and included: evaluation of oxidative stress, acrosome integrity, mitochondrial activity and DNA fragmentation. Groups were compared using a Friedman test with Bonferroni post hoc (=5%). In this study, we observed that in group I there was a decrease in acrosome integrity and mitochondrial activity, and an increase in DNA fragmentation, when compared to group C. Group AI showed an increase in acrosome integrity and mitochondrial activity when compared with group I. Based on our findings, we conclude that the vitamin E supplementation had a positive effect in protecting human spermatozoon from induced oxidative stress.Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, BrazilHosp Sao Paulo, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, BrazilHosp Sao Paulo, Sao Paulo, BrazilWeb of ScienceFAPESPCNPqFAPESP: 2015/02955-3CNPq: 306616/2013-0Wiley2020-07-20T16:30:56Z2020-07-20T16:30:56Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-http://dx.doi.org/10.1111/and.12959Andrologia. Hoboken, v. 50, n. 4, p. -, 2018.10.1111/and.129590303-4569https://repositorio.unifesp.br/handle/11600/55602WOS:000429536900018engAndrologiaHobokeninfo:eu-repo/semantics/openAccessAdami, L. N. G. [UNIFESP]Belardin, Larissa Berloffa [UNIFESP]Lima, B. T. [UNIFESP]Jeremias, J. T. [UNIFESP]Antoniassi, Mariana Pereira [UNIFESP]Okada, Fatima Kazue [UNIFESP]Bertolla, Ricardo Pimenta [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-30T11:05:21Zoai:repositorio.unifesp.br/:11600/55602Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-30T11:05:21Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| title |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| spellingShingle |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress Adami, L. N. G. [UNIFESP] antioxidant male infertility oxidative stress reactive oxygen species vitamin E |
| title_short |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| title_full |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| title_fullStr |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| title_full_unstemmed |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| title_sort |
Effect of in vitro vitamin E (alpha-tocopherol) supplementation in human spermatozoon submitted to oxidative stress |
| author |
Adami, L. N. G. [UNIFESP] |
| author_facet |
Adami, L. N. G. [UNIFESP] Belardin, Larissa Berloffa [UNIFESP] Lima, B. T. [UNIFESP] Jeremias, J. T. [UNIFESP] Antoniassi, Mariana Pereira [UNIFESP] Okada, Fatima Kazue [UNIFESP] Bertolla, Ricardo Pimenta [UNIFESP] |
| author_role |
author |
| author2 |
Belardin, Larissa Berloffa [UNIFESP] Lima, B. T. [UNIFESP] Jeremias, J. T. [UNIFESP] Antoniassi, Mariana Pereira [UNIFESP] Okada, Fatima Kazue [UNIFESP] Bertolla, Ricardo Pimenta [UNIFESP] |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Adami, L. N. G. [UNIFESP] Belardin, Larissa Berloffa [UNIFESP] Lima, B. T. [UNIFESP] Jeremias, J. T. [UNIFESP] Antoniassi, Mariana Pereira [UNIFESP] Okada, Fatima Kazue [UNIFESP] Bertolla, Ricardo Pimenta [UNIFESP] |
| dc.subject.por.fl_str_mv |
antioxidant male infertility oxidative stress reactive oxygen species vitamin E |
| topic |
antioxidant male infertility oxidative stress reactive oxygen species vitamin E |
| description |
The aim of this study was to evaluate the antioxidant effect of in vitro supplementation with vitamin E in human spermatozoon incubated with an oxidative stress inducer. In this study, semen samples from 30 patients were collected and with one aliquot we performed semen analysis according to WHO. The remaining volume was divided into four aliquots: group C: incubated with BWW medium |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2020-07-20T16:30:56Z 2020-07-20T16:30:56Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/and.12959 Andrologia. Hoboken, v. 50, n. 4, p. -, 2018. 10.1111/and.12959 0303-4569 https://repositorio.unifesp.br/handle/11600/55602 WOS:000429536900018 |
| url |
http://dx.doi.org/10.1111/and.12959 https://repositorio.unifesp.br/handle/11600/55602 |
| identifier_str_mv |
Andrologia. Hoboken, v. 50, n. 4, p. -, 2018. 10.1111/and.12959 0303-4569 WOS:000429536900018 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Andrologia |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
- |
| dc.coverage.none.fl_str_mv |
Hoboken |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268460992036864 |