Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops

• Autor(a) principal: Ferreira, Antonio José Piantino
• Data de Publicação: 1997
• Outros Autores: Elias Junior, Waldir Pereira [UNIFESP], Pelayo, Jacinta Sanchez, Giraldi, Regina [UNIFESP], Pedroso, Margareth Zabeu [UNIFESP], Scaletsky, Isabel Cristina Affonso [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1111/j.1574-695X.1997.tb01098.x; http://repositorio.unifesp.br/handle/11600/25814
• topic: Escherichia coli; shiga-like toxin; rabbit ileal loop assay

Production of Shiga toxin (Stx) in Escherichia coli strains belonging to serogroups O26, O111, and O157 was evaluated in the rabbit ileal loop assay and results were compared to those using tissue culture assays and DNA hybridization with specific probes for Stx1 and Stx2. All 14 Shiga toxin-producing E. coli strains tested provoked fluid accumulation in the rabbit intestinal loop. Eleven strains hybridized with Stx1 probe, one strain with Stx2 and two strains with both probes. Filtered culture supernatants of all E. coli strains presented cytotoxic effects in both HeLa and Vero cells, in this study, we found a strong association between the production of Stx and its effect in an animal model. This is the first description of high-level Stx-producing E. coli O111ac isolated in Brazil. (C) 1998 Published by Elsevier Science B.V. All rights reserved.