Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34385 http://dx.doi.org/10.1159/000338506 |
Resumo: | The Th1/Th2 balance represents an important factor in the pathogenesis of renal ischemia-reperfusion injury (IRI). in addition, IRI causes a systemic inflammation that can affect other tissues, such as the lungs. To investigate the ability of renal IRI to modulate pulmonary function in a specific model of allergic inflammation, C57Bl/6 mice were immunized with ovalbumin/albumen on days 0 and 7 and challenged with an ovalbumin (OA) aerosol on days 14 and 21. After 24 h of the second antigen challenge, the animals were subjected to 45 minutes of ischemia. After 24 h of reperfusion, the bronchoalveolar lavage (BAL) fluid, blood and lung tissue were collected for analysis. Serum creatinine levels increased in both allergic and non-immunized animals subjected to IRI. However, BAL analysis showed a reduction in the total cells (46%) and neutrophils (58%) compared with control allergic animals not submitted to IRI. in addition, OA challenge induced the phosphorylation of ERK and Akt and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lung homogenates. After renal IRI, the phosphorylation of ERK and expression of COX-2 and iNOS were markedly reduced; however, there was no difference in the phosphorylation of Akt between sham and ischemic OA-challenged animals. Mucus production was also reduced in allergic mice after renal IRI. IL-4, IL-5 and IL-13 were markedly down-regulated in immunized/challenged mice subjected to IRI. These results suggest that renal IRI can modulate lung allergic inflammation, probably by altering the Th1/Th2 balance and, at least in part, by changing cellular signal transduction factors. Copyright (C) 2012 S. Karger AG, Basel |
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Campanholle, GabrielaSilva, Reinaldo Correia [UNIFESP]Martins, Joilson O.Landgraf, Maristella A.Paiva, Vanessa N.Ferreira, Renaide Rodrigues [UNIFESP]Amano, Mariane T.Hiyane, Meire Ioshie [UNIFESP]Cenedeze, Marcos Antonio [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Camara, Niels Olsen Saraiva [UNIFESP]Landgraf, Richardt Gama [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T14:17:38Z2016-01-24T14:17:38Z2012-01-01Cellular Physiology and Biochemistry. Basel: Karger, v. 29, n. 3-4, p. 523-532, 2012.1015-8987http://repositorio.unifesp.br/handle/11600/34385http://dx.doi.org/10.1159/000338506WOS000302752600021.pdf10.1159/000338506WOS:000302752600021The Th1/Th2 balance represents an important factor in the pathogenesis of renal ischemia-reperfusion injury (IRI). in addition, IRI causes a systemic inflammation that can affect other tissues, such as the lungs. To investigate the ability of renal IRI to modulate pulmonary function in a specific model of allergic inflammation, C57Bl/6 mice were immunized with ovalbumin/albumen on days 0 and 7 and challenged with an ovalbumin (OA) aerosol on days 14 and 21. After 24 h of the second antigen challenge, the animals were subjected to 45 minutes of ischemia. After 24 h of reperfusion, the bronchoalveolar lavage (BAL) fluid, blood and lung tissue were collected for analysis. Serum creatinine levels increased in both allergic and non-immunized animals subjected to IRI. However, BAL analysis showed a reduction in the total cells (46%) and neutrophils (58%) compared with control allergic animals not submitted to IRI. in addition, OA challenge induced the phosphorylation of ERK and Akt and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lung homogenates. After renal IRI, the phosphorylation of ERK and expression of COX-2 and iNOS were markedly reduced; however, there was no difference in the phosphorylation of Akt between sham and ischemic OA-challenged animals. Mucus production was also reduced in allergic mice after renal IRI. IL-4, IL-5 and IL-13 were markedly down-regulated in immunized/challenged mice subjected to IRI. These results suggest that renal IRI can modulate lung allergic inflammation, probably by altering the Th1/Th2 balance and, at least in part, by changing cellular signal transduction factors. Copyright (C) 2012 S. Karger AG, BaselConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao de Auxilio aos Docentes e Alunos-UNIFESP (FADA)Complex Fluids INCTFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, BrazilUniv São Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, São Paulo, BrazilUniv São Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, BrazilUniversidade Federal de São Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, São Paulo, BrazilFAPESP: 07/07139-3FAPESP: 10/01404-0Web of Science523-532engKargerCellular Physiology and Biochemistryhttp://www.karger.com/Services/RightsPermissionsinfo:eu-repo/semantics/openAccessAsthmaRenal ischemia and reperfusionTh1/Th2 balanceLung allergic inflammationIRIModulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000302752600021.pdfapplication/pdf563162${dspace.ui.url}/bitstream/11600/34385/1/WOS000302752600021.pdffe095798b12cc90c046698f9d0dd14bcMD51open accessTEXTWOS000302752600021.pdf.txtWOS000302752600021.pdf.txtExtracted texttext/plain40720${dspace.ui.url}/bitstream/11600/34385/9/WOS000302752600021.pdf.txt275973029a24ad0372ea49d73270aadcMD59open accessTHUMBNAILWOS000302752600021.pdf.jpgWOS000302752600021.pdf.jpgIM Thumbnailimage/jpeg6793${dspace.ui.url}/bitstream/11600/34385/11/WOS000302752600021.pdf.jpg7e36766cd224f3df7db217fad6987791MD511open access11600/343852023-06-05 19:24:25.326open accessoai:repositorio.unifesp.br:11600/34385Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:24:25Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
title |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
spellingShingle |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury Campanholle, Gabriela Asthma Renal ischemia and reperfusion Th1/Th2 balance Lung allergic inflammation IRI |
title_short |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
title_full |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
title_fullStr |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
title_full_unstemmed |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
title_sort |
Modulation of Lung Allergic Response by Renal Ischemia and Reperfusion Injury |
author |
Campanholle, Gabriela |
author_facet |
Campanholle, Gabriela Silva, Reinaldo Correia [UNIFESP] Martins, Joilson O. Landgraf, Maristella A. Paiva, Vanessa N. Ferreira, Renaide Rodrigues [UNIFESP] Amano, Mariane T. Hiyane, Meire Ioshie [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Camara, Niels Olsen Saraiva [UNIFESP] Landgraf, Richardt Gama [UNIFESP] |
author_role |
author |
author2 |
Silva, Reinaldo Correia [UNIFESP] Martins, Joilson O. Landgraf, Maristella A. Paiva, Vanessa N. Ferreira, Renaide Rodrigues [UNIFESP] Amano, Mariane T. Hiyane, Meire Ioshie [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Camara, Niels Olsen Saraiva [UNIFESP] Landgraf, Richardt Gama [UNIFESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Campanholle, Gabriela Silva, Reinaldo Correia [UNIFESP] Martins, Joilson O. Landgraf, Maristella A. Paiva, Vanessa N. Ferreira, Renaide Rodrigues [UNIFESP] Amano, Mariane T. Hiyane, Meire Ioshie [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Camara, Niels Olsen Saraiva [UNIFESP] Landgraf, Richardt Gama [UNIFESP] |
dc.subject.eng.fl_str_mv |
Asthma Renal ischemia and reperfusion Th1/Th2 balance Lung allergic inflammation IRI |
topic |
Asthma Renal ischemia and reperfusion Th1/Th2 balance Lung allergic inflammation IRI |
description |
The Th1/Th2 balance represents an important factor in the pathogenesis of renal ischemia-reperfusion injury (IRI). in addition, IRI causes a systemic inflammation that can affect other tissues, such as the lungs. To investigate the ability of renal IRI to modulate pulmonary function in a specific model of allergic inflammation, C57Bl/6 mice were immunized with ovalbumin/albumen on days 0 and 7 and challenged with an ovalbumin (OA) aerosol on days 14 and 21. After 24 h of the second antigen challenge, the animals were subjected to 45 minutes of ischemia. After 24 h of reperfusion, the bronchoalveolar lavage (BAL) fluid, blood and lung tissue were collected for analysis. Serum creatinine levels increased in both allergic and non-immunized animals subjected to IRI. However, BAL analysis showed a reduction in the total cells (46%) and neutrophils (58%) compared with control allergic animals not submitted to IRI. in addition, OA challenge induced the phosphorylation of ERK and Akt and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lung homogenates. After renal IRI, the phosphorylation of ERK and expression of COX-2 and iNOS were markedly reduced; however, there was no difference in the phosphorylation of Akt between sham and ischemic OA-challenged animals. Mucus production was also reduced in allergic mice after renal IRI. IL-4, IL-5 and IL-13 were markedly down-regulated in immunized/challenged mice subjected to IRI. These results suggest that renal IRI can modulate lung allergic inflammation, probably by altering the Th1/Th2 balance and, at least in part, by changing cellular signal transduction factors. Copyright (C) 2012 S. Karger AG, Basel |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:17:38Z |
dc.date.available.fl_str_mv |
2016-01-24T14:17:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Cellular Physiology and Biochemistry. Basel: Karger, v. 29, n. 3-4, p. 523-532, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34385 http://dx.doi.org/10.1159/000338506 |
dc.identifier.issn.none.fl_str_mv |
1015-8987 |
dc.identifier.file.none.fl_str_mv |
WOS000302752600021.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1159/000338506 |
dc.identifier.wos.none.fl_str_mv |
WOS:000302752600021 |
identifier_str_mv |
Cellular Physiology and Biochemistry. Basel: Karger, v. 29, n. 3-4, p. 523-532, 2012. 1015-8987 WOS000302752600021.pdf 10.1159/000338506 WOS:000302752600021 |
url |
http://repositorio.unifesp.br/handle/11600/34385 http://dx.doi.org/10.1159/000338506 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Cellular Physiology and Biochemistry |
dc.rights.driver.fl_str_mv |
http://www.karger.com/Services/RightsPermissions info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.karger.com/Services/RightsPermissions |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
523-532 |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
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