The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Detalhes bibliográficos
Autor(a) principal: Castro,Angela Potter de
Data de Publicação: 2012
Outros Autores: Castro Junior,Miguel Angelo Martins de, Lauz,Susi, Facin,Emilio, Simões,Manuel de Jesus, Fagundes,Djalma José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100009
Resumo: PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
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spelling The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbitsAcetylcysteineLungReperfusionIschemiaLiverRabbitsPURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100009Acta Cirúrgica Brasileira v.27 n.1 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012000100009info:eu-repo/semantics/openAccessCastro,Angela Potter deCastro Junior,Miguel Angelo Martins deLauz,SusiFacin,EmilioSimões,Manuel de JesusFagundes,Djalma Joséeng2011-12-19T00:00:00Zoai:scielo:S0102-86502012000100009Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2011-12-19T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
spellingShingle The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
Castro,Angela Potter de
Acetylcysteine
Lung
Reperfusion
Ischemia
Liver
Rabbits
title_short The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_full The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_fullStr The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_full_unstemmed The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_sort The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
author Castro,Angela Potter de
author_facet Castro,Angela Potter de
Castro Junior,Miguel Angelo Martins de
Lauz,Susi
Facin,Emilio
Simões,Manuel de Jesus
Fagundes,Djalma José
author_role author
author2 Castro Junior,Miguel Angelo Martins de
Lauz,Susi
Facin,Emilio
Simões,Manuel de Jesus
Fagundes,Djalma José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Castro,Angela Potter de
Castro Junior,Miguel Angelo Martins de
Lauz,Susi
Facin,Emilio
Simões,Manuel de Jesus
Fagundes,Djalma José
dc.subject.por.fl_str_mv Acetylcysteine
Lung
Reperfusion
Ischemia
Liver
Rabbits
topic Acetylcysteine
Lung
Reperfusion
Ischemia
Liver
Rabbits
description PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502012000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.27 n.1 2012
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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