Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.virol.2008.08.029 http://repositorio.unifesp.br/handle/11600/31045 |
Resumo: | One of most intriguing features of the HIV-1 subtype B epidemic in Brazil is the high frequency of isolates exhibiting tryptophan (W) in the tetramer (GWGR) at the tip of the V3 loop. We observed that the frequencies of glutamic and aspartic acids at site 25 of the V3 loop are quite distinct in GWGR isolates compared with viruses with other tetramers. the basic amino acids at sites 11 and 25 of V3 are strongly linked with CCR5-to-CXCR4 coreceptor shift. We therefore predicted phenotype usage and found that GWGR isolates are exclusively CCR5-using. Further evidence of this came from intrahost sequences, where basic amino acid substitutions at sites 11 and 25 emerged only in isolates presenting a tryptophan-to-glycine replacement at the tetramer of the V3. in addition, modeled 3D-structures of the V3 loop of GWGR and GGGR in intrahost viruses differ essentially in the binding region of the coreceptor. (C) 2008 Elsevier Inc. All rights reserved. |
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Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loopHIV-1V3 loopGWGRCoreceptorCCR5CXCR43D-structureOne of most intriguing features of the HIV-1 subtype B epidemic in Brazil is the high frequency of isolates exhibiting tryptophan (W) in the tetramer (GWGR) at the tip of the V3 loop. We observed that the frequencies of glutamic and aspartic acids at site 25 of the V3 loop are quite distinct in GWGR isolates compared with viruses with other tetramers. the basic amino acids at sites 11 and 25 of V3 are strongly linked with CCR5-to-CXCR4 coreceptor shift. We therefore predicted phenotype usage and found that GWGR isolates are exclusively CCR5-using. Further evidence of this came from intrahost sequences, where basic amino acid substitutions at sites 11 and 25 emerged only in isolates presenting a tryptophan-to-glycine replacement at the tetramer of the V3. in addition, modeled 3D-structures of the V3 loop of GWGR and GGGR in intrahost viruses differ essentially in the binding region of the coreceptor. (C) 2008 Elsevier Inc. All rights reserved.Universidade Federal de São Paulo, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04039032 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 04/10372-3FAPESP: 07/52841-8Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Leal, Elcio de Souza [UNIFESP]Silva, Wilson Pereira [UNIFESP]Sucupira, Maria Cecília Araripe [UNIFESP]Janini, Luiz Mário Ramos [UNIFESP]Diaz, Ricardo Sobhie [UNIFESP]2016-01-24T13:51:54Z2016-01-24T13:51:54Z2008-11-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion222-229application/pdfhttp://dx.doi.org/10.1016/j.virol.2008.08.029Virology. San Diego: Academic Press Inc Elsevier Science, v. 381, n. 2, p. 222-229, 2008.10.1016/j.virol.2008.08.029WOS000260841500009.pdf0042-6822http://repositorio.unifesp.br/handle/11600/31045WOS:000260841500009engVirologyinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T00:30:10Zoai:repositorio.unifesp.br/:11600/31045Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T00:30:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
title |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
spellingShingle |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop Leal, Elcio de Souza [UNIFESP] HIV-1 V3 loop GWGR Coreceptor CCR5 CXCR4 3D-structure |
title_short |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
title_full |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
title_fullStr |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
title_full_unstemmed |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
title_sort |
Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop |
author |
Leal, Elcio de Souza [UNIFESP] |
author_facet |
Leal, Elcio de Souza [UNIFESP] Silva, Wilson Pereira [UNIFESP] Sucupira, Maria Cecília Araripe [UNIFESP] Janini, Luiz Mário Ramos [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] |
author_role |
author |
author2 |
Silva, Wilson Pereira [UNIFESP] Sucupira, Maria Cecília Araripe [UNIFESP] Janini, Luiz Mário Ramos [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Leal, Elcio de Souza [UNIFESP] Silva, Wilson Pereira [UNIFESP] Sucupira, Maria Cecília Araripe [UNIFESP] Janini, Luiz Mário Ramos [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] |
dc.subject.por.fl_str_mv |
HIV-1 V3 loop GWGR Coreceptor CCR5 CXCR4 3D-structure |
topic |
HIV-1 V3 loop GWGR Coreceptor CCR5 CXCR4 3D-structure |
description |
One of most intriguing features of the HIV-1 subtype B epidemic in Brazil is the high frequency of isolates exhibiting tryptophan (W) in the tetramer (GWGR) at the tip of the V3 loop. We observed that the frequencies of glutamic and aspartic acids at site 25 of the V3 loop are quite distinct in GWGR isolates compared with viruses with other tetramers. the basic amino acids at sites 11 and 25 of V3 are strongly linked with CCR5-to-CXCR4 coreceptor shift. We therefore predicted phenotype usage and found that GWGR isolates are exclusively CCR5-using. Further evidence of this came from intrahost sequences, where basic amino acid substitutions at sites 11 and 25 emerged only in isolates presenting a tryptophan-to-glycine replacement at the tetramer of the V3. in addition, modeled 3D-structures of the V3 loop of GWGR and GGGR in intrahost viruses differ essentially in the binding region of the coreceptor. (C) 2008 Elsevier Inc. All rights reserved. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-11-25 2016-01-24T13:51:54Z 2016-01-24T13:51:54Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.virol.2008.08.029 Virology. San Diego: Academic Press Inc Elsevier Science, v. 381, n. 2, p. 222-229, 2008. 10.1016/j.virol.2008.08.029 WOS000260841500009.pdf 0042-6822 http://repositorio.unifesp.br/handle/11600/31045 WOS:000260841500009 |
url |
http://dx.doi.org/10.1016/j.virol.2008.08.029 http://repositorio.unifesp.br/handle/11600/31045 |
identifier_str_mv |
Virology. San Diego: Academic Press Inc Elsevier Science, v. 381, n. 2, p. 222-229, 2008. 10.1016/j.virol.2008.08.029 WOS000260841500009.pdf 0042-6822 WOS:000260841500009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Virology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
222-229 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268367073181696 |