hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions

Detalhes bibliográficos
Autor(a) principal: Silva, Tanielly Cristina Raiol
Data de Publicação: 2012
Outros Autores: Leal, Mariana Ferreira [UNIFESP], Calcagno, Danielle Queiroz [UNIFESP], Souza, Carolina Rosal Teixeira de, Khayat, Andre Salim, Santos, Ney Pereira Carneiro dos, Montenegro, Raquel Carvalho, Rabenhorst, Silvia Helena Barem, Nascimento, Mayara Quaresma, Assumpcao, Paulo Pimentel, Smith, Marilia de Arruda Cardoso [UNIFESP], Burbano, Rommel Rodriguez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1186/1471-230X-12-85
http://repositorio.unifesp.br/handle/11600/35090
Resumo: Background: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.
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spelling hTERT, MYC and TP53 deregulation in gastric preneoplastic lesionshTERTMYCTP53Gastric carcinogenesisPrecancerous lesionsBackground: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.Universidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, BrazilFed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66073000 Belem, PA, BrazilFed Univ Para, Hosp Univ Joao de Barros Barreto, Unidade Alta Complexidade Oncol, BR-60673000 Belem, PA, BrazilUniv Fed Ceara, Escola Med, Dept Patol & Med Forense, Genet Mol Lab, BR-60020181 Fortaleza, CE, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)CNPq: 302774/2009-2CNPq: 301609/2007-1Biomed Central LtdUniversidade Federal de São Paulo (UNIFESP)Fed Univ ParaUniv Fed CearaSilva, Tanielly Cristina RaiolLeal, Mariana Ferreira [UNIFESP]Calcagno, Danielle Queiroz [UNIFESP]Souza, Carolina Rosal Teixeira deKhayat, Andre SalimSantos, Ney Pereira Carneiro dosMontenegro, Raquel CarvalhoRabenhorst, Silvia Helena BaremNascimento, Mayara QuaresmaAssumpcao, Paulo PimentelSmith, Marilia de Arruda Cardoso [UNIFESP]Burbano, Rommel Rodriguez2016-01-24T14:27:28Z2016-01-24T14:27:28Z2012-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8application/pdfhttp://dx.doi.org/10.1186/1471-230X-12-85Bmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012.10.1186/1471-230X-12-85WOS000310335300001.pdf1471-230Xhttp://repositorio.unifesp.br/handle/11600/35090WOS:000310335300001engBmc Gastroenterologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T13:39:25Zoai:repositorio.unifesp.br/:11600/35090Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T13:39:25Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
title hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
spellingShingle hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
Silva, Tanielly Cristina Raiol
hTERT
MYC
TP53
Gastric carcinogenesis
Precancerous lesions
title_short hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
title_full hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
title_fullStr hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
title_full_unstemmed hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
title_sort hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
author Silva, Tanielly Cristina Raiol
author_facet Silva, Tanielly Cristina Raiol
Leal, Mariana Ferreira [UNIFESP]
Calcagno, Danielle Queiroz [UNIFESP]
Souza, Carolina Rosal Teixeira de
Khayat, Andre Salim
Santos, Ney Pereira Carneiro dos
Montenegro, Raquel Carvalho
Rabenhorst, Silvia Helena Barem
Nascimento, Mayara Quaresma
Assumpcao, Paulo Pimentel
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodriguez
author_role author
author2 Leal, Mariana Ferreira [UNIFESP]
Calcagno, Danielle Queiroz [UNIFESP]
Souza, Carolina Rosal Teixeira de
Khayat, Andre Salim
Santos, Ney Pereira Carneiro dos
Montenegro, Raquel Carvalho
Rabenhorst, Silvia Helena Barem
Nascimento, Mayara Quaresma
Assumpcao, Paulo Pimentel
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodriguez
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Univ Fed Ceara
dc.contributor.author.fl_str_mv Silva, Tanielly Cristina Raiol
Leal, Mariana Ferreira [UNIFESP]
Calcagno, Danielle Queiroz [UNIFESP]
Souza, Carolina Rosal Teixeira de
Khayat, Andre Salim
Santos, Ney Pereira Carneiro dos
Montenegro, Raquel Carvalho
Rabenhorst, Silvia Helena Barem
Nascimento, Mayara Quaresma
Assumpcao, Paulo Pimentel
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodriguez
dc.subject.por.fl_str_mv hTERT
MYC
TP53
Gastric carcinogenesis
Precancerous lesions
topic hTERT
MYC
TP53
Gastric carcinogenesis
Precancerous lesions
description Background: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.
publishDate 2012
dc.date.none.fl_str_mv 2012-07-06
2016-01-24T14:27:28Z
2016-01-24T14:27:28Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1471-230X-12-85
Bmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012.
10.1186/1471-230X-12-85
WOS000310335300001.pdf
1471-230X
http://repositorio.unifesp.br/handle/11600/35090
WOS:000310335300001
url http://dx.doi.org/10.1186/1471-230X-12-85
http://repositorio.unifesp.br/handle/11600/35090
identifier_str_mv Bmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012.
10.1186/1471-230X-12-85
WOS000310335300001.pdf
1471-230X
WOS:000310335300001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bmc Gastroenterology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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