hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-230X-12-85 http://repositorio.unifesp.br/handle/11600/35090 |
Resumo: | Background: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation. |
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hTERT, MYC and TP53 deregulation in gastric preneoplastic lesionshTERTMYCTP53Gastric carcinogenesisPrecancerous lesionsBackground: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation.Universidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, BrazilFed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66073000 Belem, PA, BrazilFed Univ Para, Hosp Univ Joao de Barros Barreto, Unidade Alta Complexidade Oncol, BR-60673000 Belem, PA, BrazilUniv Fed Ceara, Escola Med, Dept Patol & Med Forense, Genet Mol Lab, BR-60020181 Fortaleza, CE, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, BR-04023900 São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)CNPq: 302774/2009-2CNPq: 301609/2007-1Biomed Central LtdUniversidade Federal de São Paulo (UNIFESP)Fed Univ ParaUniv Fed CearaSilva, Tanielly Cristina RaiolLeal, Mariana Ferreira [UNIFESP]Calcagno, Danielle Queiroz [UNIFESP]Souza, Carolina Rosal Teixeira deKhayat, Andre SalimSantos, Ney Pereira Carneiro dosMontenegro, Raquel CarvalhoRabenhorst, Silvia Helena BaremNascimento, Mayara QuaresmaAssumpcao, Paulo PimentelSmith, Marilia de Arruda Cardoso [UNIFESP]Burbano, Rommel Rodriguez2016-01-24T14:27:28Z2016-01-24T14:27:28Z2012-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8application/pdfhttp://dx.doi.org/10.1186/1471-230X-12-85Bmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012.10.1186/1471-230X-12-85WOS000310335300001.pdf1471-230Xhttp://repositorio.unifesp.br/handle/11600/35090WOS:000310335300001engBmc Gastroenterologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T13:39:25Zoai:repositorio.unifesp.br/:11600/35090Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T13:39:25Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
title |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
spellingShingle |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions Silva, Tanielly Cristina Raiol hTERT MYC TP53 Gastric carcinogenesis Precancerous lesions |
title_short |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
title_full |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
title_fullStr |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
title_full_unstemmed |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
title_sort |
hTERT, MYC and TP53 deregulation in gastric preneoplastic lesions |
author |
Silva, Tanielly Cristina Raiol |
author_facet |
Silva, Tanielly Cristina Raiol Leal, Mariana Ferreira [UNIFESP] Calcagno, Danielle Queiroz [UNIFESP] Souza, Carolina Rosal Teixeira de Khayat, Andre Salim Santos, Ney Pereira Carneiro dos Montenegro, Raquel Carvalho Rabenhorst, Silvia Helena Barem Nascimento, Mayara Quaresma Assumpcao, Paulo Pimentel Smith, Marilia de Arruda Cardoso [UNIFESP] Burbano, Rommel Rodriguez |
author_role |
author |
author2 |
Leal, Mariana Ferreira [UNIFESP] Calcagno, Danielle Queiroz [UNIFESP] Souza, Carolina Rosal Teixeira de Khayat, Andre Salim Santos, Ney Pereira Carneiro dos Montenegro, Raquel Carvalho Rabenhorst, Silvia Helena Barem Nascimento, Mayara Quaresma Assumpcao, Paulo Pimentel Smith, Marilia de Arruda Cardoso [UNIFESP] Burbano, Rommel Rodriguez |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fed Univ Para Univ Fed Ceara |
dc.contributor.author.fl_str_mv |
Silva, Tanielly Cristina Raiol Leal, Mariana Ferreira [UNIFESP] Calcagno, Danielle Queiroz [UNIFESP] Souza, Carolina Rosal Teixeira de Khayat, Andre Salim Santos, Ney Pereira Carneiro dos Montenegro, Raquel Carvalho Rabenhorst, Silvia Helena Barem Nascimento, Mayara Quaresma Assumpcao, Paulo Pimentel Smith, Marilia de Arruda Cardoso [UNIFESP] Burbano, Rommel Rodriguez |
dc.subject.por.fl_str_mv |
hTERT MYC TP53 Gastric carcinogenesis Precancerous lesions |
topic |
hTERT MYC TP53 Gastric carcinogenesis Precancerous lesions |
description |
Background: Gastric cancer is a serious public health problem in Northern Brazil and in the world due to its high incidence and mortality. Despite the severity of the disease, more research is needed to better understand the molecular events involved in this intestinal-type gastric carcinogenesis process. Since precancerous lesions precede intestinal-type gastric cancer, here, we evaluated the hTERT, MYC, and TP53 mRNA and protein expression, as well as TP33 copy number, in gastric preneoplastic lesions.Methods: We evaluated 19 superficial gastritis, 18 atrophic gastritis, and 18 intestinal metaplasia from cancer-free individuals of Northern Brazil. Quantitative reverse transcription PCR was used to analyze the mRNA expression and immunohistochemical methods were used to assess protein immunoreactivity in tissue samples. the number of TP53 gene copies was investigated in gastric diseases by quantitative PCR.Results: We observed hTERT, MYC, and p53 immunoreactivity only in intestinal metaplasia samples. the immunoreactivity of these proteins was strongly associated with each other. A significantly higher MYC mRNA expression was observed in intestinal metaplasia compared to gastritis samples. Loss of TP53 was also only detected in intestinal metaplasia specimens.Conclusions: We demonstrated that hTERT, MYC, and TP53 are deregulated in intestinal metaplasia of individuals from Northern Brazil and these alterations may facilitate tumor initiation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-06 2016-01-24T14:27:28Z 2016-01-24T14:27:28Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-230X-12-85 Bmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012. 10.1186/1471-230X-12-85 WOS000310335300001.pdf 1471-230X http://repositorio.unifesp.br/handle/11600/35090 WOS:000310335300001 |
url |
http://dx.doi.org/10.1186/1471-230X-12-85 http://repositorio.unifesp.br/handle/11600/35090 |
identifier_str_mv |
Bmc Gastroenterology. London: Biomed Central Ltd, v. 12, 8 p., 2012. 10.1186/1471-230X-12-85 WOS000310335300001.pdf 1471-230X WOS:000310335300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Gastroenterology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268316455272448 |