Characterization of an animal model of severe sepsis associated with respiratory dysfunction

Detalhes bibliográficos
Autor(a) principal: Azevedo, Luciano Cesar Pontes [UNIFESP]
Data de Publicação: 2007
Outros Autores: Park, Marcelo, Noritomi, Danilo Teixeira, Maciel, Alexandre Toledo, Brunialti, Milena Karina Coló [UNIFESP], Salomão, Reinaldo [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1807-59322007000400017
http://repositorio.unifesp.br/handle/11600/3443
Resumo: PURPOSE: Pathophysiological studies in humans regarding sepsis are difficult to perform due to ethical and methodological concerns. In this context, animal models of sepsis can be useful to better understand this condition and to test therapeutic strategies. The purpose of this study was to characterize a feasible and clinically relevant model of sepsis in pigs that could be useful for testing different therapeutic interventions. METHODS: 5 White Large pigs were anesthetized, arterial and pulmonary catheters were introduced, and sepsis was induced by fecal peritonitis. Several biochemical indicators of organ dysfunction and infectious parameters were measured. The pigs were monitored until death, when fragments of organs were removed for pathology. Three animals without peritonitis served as controls and were sacrificed 24 hours after surgery without developing significant changes in organ function. RESULTS: Septic pigs survived 17 hours on average (range, 16-18 h), and Escherichia coli was recovered from blood cultures. They developed a significant decrease in left ventricular work and a nonsignificant reduction in mixed venous oxygen saturation. Respiratory dysfunction was characterized by a decrease in the PaO2/FiO2 ratio and respiratory compliance. Pathology of the lungs revealed areas of pulmonary collapse, hemorrhage, pulmonary congestion, and discrete neutrophil infiltrate. CONCLUSIONS: Fecal peritonitis in pigs is a clinically relevant model of sepsis associated with acute lung injury without direct pulmonary insult. This model may prove to be useful for studying pathogenic aspects of secondary lung injury as well as for validating ventilatory or pharmacologic interventions.
id UFSP_1149ca51166571c3b15910ec560d487b
oai_identifier_str oai:repositorio.unifesp.br/:11600/3443
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Characterization of an animal model of severe sepsis associated with respiratory dysfunctionSepsisAcute lung injuryPigsAnimal modelsPeritonitisSepseLesão pulmonar agudaPorcosModelos animais e peritonitePURPOSE: Pathophysiological studies in humans regarding sepsis are difficult to perform due to ethical and methodological concerns. In this context, animal models of sepsis can be useful to better understand this condition and to test therapeutic strategies. The purpose of this study was to characterize a feasible and clinically relevant model of sepsis in pigs that could be useful for testing different therapeutic interventions. METHODS: 5 White Large pigs were anesthetized, arterial and pulmonary catheters were introduced, and sepsis was induced by fecal peritonitis. Several biochemical indicators of organ dysfunction and infectious parameters were measured. The pigs were monitored until death, when fragments of organs were removed for pathology. Three animals without peritonitis served as controls and were sacrificed 24 hours after surgery without developing significant changes in organ function. RESULTS: Septic pigs survived 17 hours on average (range, 16-18 h), and Escherichia coli was recovered from blood cultures. They developed a significant decrease in left ventricular work and a nonsignificant reduction in mixed venous oxygen saturation. Respiratory dysfunction was characterized by a decrease in the PaO2/FiO2 ratio and respiratory compliance. Pathology of the lungs revealed areas of pulmonary collapse, hemorrhage, pulmonary congestion, and discrete neutrophil infiltrate. CONCLUSIONS: Fecal peritonitis in pigs is a clinically relevant model of sepsis associated with acute lung injury without direct pulmonary insult. This model may prove to be useful for studying pathogenic aspects of secondary lung injury as well as for validating ventilatory or pharmacologic interventions.PROPOSTA: Estudos sobre sepse envolvendo sua fisiopatologia são difíceis de serem realizados devido a razões éticas e metodológicas. Neste sentido, modelos animais criam oportunidades de estudos para entender a fisiopatologia e testar estratégias terapêuticas. O objetivo deste estudo foi criar um modelo relevante de choque séptico em porcos para testar e entender diferentes intervenções. MÉTODOS: 5 porcos da raça White Large foram anestesiados e monitorizados com uma linha arterial e um cateter de artéria pulmonar. Uma peritonite fecal foi induzida através de laparotomia. Marcadores de disfunções orgânicas e infecciosos foram mensurados. Todos porcos evoluíram até a morte e amostras de órgãos foram coletadas para exame anátomo patológico. Três animais controles com o mesmo preparo cirúrgico e sem peritonite foram sacrificados após 24 horas de evolução, sem desenvolver mudanças significativas nas funções orgânicas. RESULTADOS: Os animais séptico sobreviveram na média 17 horas (16 - 18h), e Escherichia coli foi cultivada nas amostras de sangue. Os animais sépticos evoluíram com redução do trabalho de ventrículo esquerdo. A disfunção respiratória foi caracterizada por uma redução na relação PaO2/FiO2 e na complacência respiratória. A anatomia patológica dos pulmões revelou colapso pulmonar, hemorragia, congestão e infiltrado neutrofílico. CONCLUSÕES: A peritonite fecal em porcos é um modelo de choque séptico clinicamente relevante e associada a uma lesão pulmonar sem um insulto direto. Este é um modelo que pode ser utilizado para estudar aspectos fisiopatológicos das lesões pulmonares secundárias, assim como para estudar intervenções ventilatórias ou farmacológicas.Hospital Sírio-Libanês Research and Education InstituteHospital das Clínicas Medical Intensive Care UnitFederal University of São Paulo Immunology LaboratoryUNIFESP, Immunology LaboratorySciELOFaculdade de Medicina / USPHospital Sírio-Libanês Research and Education InstituteHospital das Clínicas Medical Intensive Care UnitUniversidade Federal de São Paulo (UNIFESP)Azevedo, Luciano Cesar Pontes [UNIFESP]Park, MarceloNoritomi, Danilo TeixeiraMaciel, Alexandre ToledoBrunialti, Milena Karina Coló [UNIFESP]Salomão, Reinaldo [UNIFESP]2015-06-14T13:36:37Z2015-06-14T13:36:37Z2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion491-498application/pdfhttp://dx.doi.org/10.1590/S1807-59322007000400017Clinics. Faculdade de Medicina / USP, v. 62, n. 4, p. 491-498, 2007.10.1590/S1807-59322007000400017S1807-59322007000400017.pdf1807-5932S1807-59322007000400017http://repositorio.unifesp.br/handle/11600/3443WOS:000254319800017WOS:000254319800017engClinicsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T10:02:51Zoai:repositorio.unifesp.br/:11600/3443Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T10:02:51Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Characterization of an animal model of severe sepsis associated with respiratory dysfunction
title Characterization of an animal model of severe sepsis associated with respiratory dysfunction
spellingShingle Characterization of an animal model of severe sepsis associated with respiratory dysfunction
Azevedo, Luciano Cesar Pontes [UNIFESP]
Sepsis
Acute lung injury
Pigs
Animal models
Peritonitis
Sepse
Lesão pulmonar aguda
Porcos
Modelos animais e peritonite
title_short Characterization of an animal model of severe sepsis associated with respiratory dysfunction
title_full Characterization of an animal model of severe sepsis associated with respiratory dysfunction
title_fullStr Characterization of an animal model of severe sepsis associated with respiratory dysfunction
title_full_unstemmed Characterization of an animal model of severe sepsis associated with respiratory dysfunction
title_sort Characterization of an animal model of severe sepsis associated with respiratory dysfunction
author Azevedo, Luciano Cesar Pontes [UNIFESP]
author_facet Azevedo, Luciano Cesar Pontes [UNIFESP]
Park, Marcelo
Noritomi, Danilo Teixeira
Maciel, Alexandre Toledo
Brunialti, Milena Karina Coló [UNIFESP]
Salomão, Reinaldo [UNIFESP]
author_role author
author2 Park, Marcelo
Noritomi, Danilo Teixeira
Maciel, Alexandre Toledo
Brunialti, Milena Karina Coló [UNIFESP]
Salomão, Reinaldo [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Hospital Sírio-Libanês Research and Education Institute
Hospital das Clínicas Medical Intensive Care Unit
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Azevedo, Luciano Cesar Pontes [UNIFESP]
Park, Marcelo
Noritomi, Danilo Teixeira
Maciel, Alexandre Toledo
Brunialti, Milena Karina Coló [UNIFESP]
Salomão, Reinaldo [UNIFESP]
dc.subject.por.fl_str_mv Sepsis
Acute lung injury
Pigs
Animal models
Peritonitis
Sepse
Lesão pulmonar aguda
Porcos
Modelos animais e peritonite
topic Sepsis
Acute lung injury
Pigs
Animal models
Peritonitis
Sepse
Lesão pulmonar aguda
Porcos
Modelos animais e peritonite
description PURPOSE: Pathophysiological studies in humans regarding sepsis are difficult to perform due to ethical and methodological concerns. In this context, animal models of sepsis can be useful to better understand this condition and to test therapeutic strategies. The purpose of this study was to characterize a feasible and clinically relevant model of sepsis in pigs that could be useful for testing different therapeutic interventions. METHODS: 5 White Large pigs were anesthetized, arterial and pulmonary catheters were introduced, and sepsis was induced by fecal peritonitis. Several biochemical indicators of organ dysfunction and infectious parameters were measured. The pigs were monitored until death, when fragments of organs were removed for pathology. Three animals without peritonitis served as controls and were sacrificed 24 hours after surgery without developing significant changes in organ function. RESULTS: Septic pigs survived 17 hours on average (range, 16-18 h), and Escherichia coli was recovered from blood cultures. They developed a significant decrease in left ventricular work and a nonsignificant reduction in mixed venous oxygen saturation. Respiratory dysfunction was characterized by a decrease in the PaO2/FiO2 ratio and respiratory compliance. Pathology of the lungs revealed areas of pulmonary collapse, hemorrhage, pulmonary congestion, and discrete neutrophil infiltrate. CONCLUSIONS: Fecal peritonitis in pigs is a clinically relevant model of sepsis associated with acute lung injury without direct pulmonary insult. This model may prove to be useful for studying pathogenic aspects of secondary lung injury as well as for validating ventilatory or pharmacologic interventions.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
2015-06-14T13:36:37Z
2015-06-14T13:36:37Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1807-59322007000400017
Clinics. Faculdade de Medicina / USP, v. 62, n. 4, p. 491-498, 2007.
10.1590/S1807-59322007000400017
S1807-59322007000400017.pdf
1807-5932
S1807-59322007000400017
http://repositorio.unifesp.br/handle/11600/3443
WOS:000254319800017
WOS:000254319800017
url http://dx.doi.org/10.1590/S1807-59322007000400017
http://repositorio.unifesp.br/handle/11600/3443
identifier_str_mv Clinics. Faculdade de Medicina / USP, v. 62, n. 4, p. 491-498, 2007.
10.1590/S1807-59322007000400017
S1807-59322007000400017.pdf
1807-5932
S1807-59322007000400017
WOS:000254319800017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 491-498
application/pdf
dc.publisher.none.fl_str_mv Faculdade de Medicina / USP
publisher.none.fl_str_mv Faculdade de Medicina / USP
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268363864539136