Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/4512 http://dx.doi.org/10.1590/S1677-55382008000400013 |
Resumo: | PURPOSE: We reproduced a non-bacterial experimental model to assess bladder inflammation and urinary glycosaminoglycans (GAG) excretion and examined the effect of dimethyl sulfoxide (DMSO). MATERIALS AND METHODS: Female rats were instilled with either protamine sulfate (PS groups) or sterile saline (control groups). At different days after the procedure, 24 h urine and bladder samples were obtained. Urinary levels of hyaluronic acid (HA) and sulfated glycosaminoglycans (S-GAG) were determined. Also to evaluate the effect of DMSO animals were instilled with either 50% DMSO or saline 6 hours after PS instillation. To evaluate the effect of DMSO in healthy bladders, rats were instilled with 50% DMSO and controls with saline. RESULTS: In the PS groups, bladder inflammation was observed, with polymorphonuclear cells during the first days and lymphomononuclear in the last days. HA and S-GAG had 2 peaks of urinary excretion, at the 1st and 7th day after PS injection. DMSO significantly reduced bladder inflammation. In contrast, in healthy bladders, DMSO produced mild inflammation and an increase in urinary HA levels after 1 and 7 days and an increase of S-GAG level in 7 days. Animals instilled with PS and treated with DMSO had significantly reduced levels of urinary HA only at the 1st day. Urinary S-GAG/Cr levels were similar in all groups. CONCLUSIONS: Increased urinary levels of GAG were associated with bladder inflammation in a PS-induced cystitis model. DMSO significantly reduced the inflammatory process after urothelial injury. Conversely, this drug provoked mild inflammation in normal mucosa. DMSO treatment was shown to influence urinary HA excretion. |
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Soler, Roberto [UNIFESP]Bruschini, HomeroTruzzi, Jose Carlos [UNIFESP]Martins, Joao R. [UNIFESP]Câmara, Niels Olsen Saraiva [UNIFESP]Alves, Maria Teresa de Seixas [UNIFESP]Leite, Kátia Ramos Moreira [UNIFESP]Nader, Helena Bonciani [UNIFESP]Srougi, Miguel [UNIFESP]Ortiz, Valdemar [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2015-06-14T13:38:40Z2015-06-14T13:38:40Z2008-08-01International braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 4, p. 503-511, 2008.1677-5538http://repositorio.unifesp.br/handle/11600/4512http://dx.doi.org/10.1590/S1677-55382008000400013S1677-55382008000400013.pdfS1677-5538200800040001310.1590/S1677-55382008000400013WOS:000207705100026PURPOSE: We reproduced a non-bacterial experimental model to assess bladder inflammation and urinary glycosaminoglycans (GAG) excretion and examined the effect of dimethyl sulfoxide (DMSO). MATERIALS AND METHODS: Female rats were instilled with either protamine sulfate (PS groups) or sterile saline (control groups). At different days after the procedure, 24 h urine and bladder samples were obtained. Urinary levels of hyaluronic acid (HA) and sulfated glycosaminoglycans (S-GAG) were determined. Also to evaluate the effect of DMSO animals were instilled with either 50% DMSO or saline 6 hours after PS instillation. To evaluate the effect of DMSO in healthy bladders, rats were instilled with 50% DMSO and controls with saline. RESULTS: In the PS groups, bladder inflammation was observed, with polymorphonuclear cells during the first days and lymphomononuclear in the last days. HA and S-GAG had 2 peaks of urinary excretion, at the 1st and 7th day after PS injection. DMSO significantly reduced bladder inflammation. In contrast, in healthy bladders, DMSO produced mild inflammation and an increase in urinary HA levels after 1 and 7 days and an increase of S-GAG level in 7 days. Animals instilled with PS and treated with DMSO had significantly reduced levels of urinary HA only at the 1st day. Urinary S-GAG/Cr levels were similar in all groups. CONCLUSIONS: Increased urinary levels of GAG were associated with bladder inflammation in a PS-induced cystitis model. DMSO significantly reduced the inflammatory process after urothelial injury. Conversely, this drug provoked mild inflammation in normal mucosa. DMSO treatment was shown to influence urinary HA excretion.Federal University of São Paulo Division of UrologyFederal University of São Paulo Division of Molecular BiologyFederal University of São Paulo Division of NephrologyFederal University of São Paulo Division of PathologyUniversity of São Paulo Division of UrologyUNIFESP, Division of UrologyUNIFESP, Division of Molecular BiologyUNIFESP, Division of NephrologyUNIFESP, Division of PathologySciELO503-511engSociedade Brasileira de UrologiaInternational braz j urolcystitisdimethyl sulfoxideglycosaminoglycansratsprotaminesUrinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1677-55382008000400013.pdfapplication/pdf410089${dspace.ui.url}/bitstream/11600/4512/1/S1677-55382008000400013.pdf87993ba299cb90509e258b10d47af6ceMD51open accessTEXTS1677-55382008000400013.pdf.txtS1677-55382008000400013.pdf.txtExtracted texttext/plain2878${dspace.ui.url}/bitstream/11600/4512/9/S1677-55382008000400013.pdf.txt560ae73b5e8a523901ecd025db23e4e1MD59open accessTHUMBNAILS1677-55382008000400013.pdf.jpgS1677-55382008000400013.pdf.jpgIM Thumbnailimage/jpeg6351${dspace.ui.url}/bitstream/11600/4512/11/S1677-55382008000400013.pdf.jpg013ac334049297d24d52431a4dfb2646MD511open access11600/45122023-06-05 19:34:00.348open accessoai:repositorio.unifesp.br:11600/4512Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
title |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
spellingShingle |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis Soler, Roberto [UNIFESP] cystitis dimethyl sulfoxide glycosaminoglycans rats protamines |
title_short |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
title_full |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
title_fullStr |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
title_full_unstemmed |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
title_sort |
Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis |
author |
Soler, Roberto [UNIFESP] |
author_facet |
Soler, Roberto [UNIFESP] Bruschini, Homero Truzzi, Jose Carlos [UNIFESP] Martins, Joao R. [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] Alves, Maria Teresa de Seixas [UNIFESP] Leite, Kátia Ramos Moreira [UNIFESP] Nader, Helena Bonciani [UNIFESP] Srougi, Miguel [UNIFESP] Ortiz, Valdemar [UNIFESP] |
author_role |
author |
author2 |
Bruschini, Homero Truzzi, Jose Carlos [UNIFESP] Martins, Joao R. [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] Alves, Maria Teresa de Seixas [UNIFESP] Leite, Kátia Ramos Moreira [UNIFESP] Nader, Helena Bonciani [UNIFESP] Srougi, Miguel [UNIFESP] Ortiz, Valdemar [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Soler, Roberto [UNIFESP] Bruschini, Homero Truzzi, Jose Carlos [UNIFESP] Martins, Joao R. [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] Alves, Maria Teresa de Seixas [UNIFESP] Leite, Kátia Ramos Moreira [UNIFESP] Nader, Helena Bonciani [UNIFESP] Srougi, Miguel [UNIFESP] Ortiz, Valdemar [UNIFESP] |
dc.subject.eng.fl_str_mv |
cystitis dimethyl sulfoxide glycosaminoglycans rats protamines |
topic |
cystitis dimethyl sulfoxide glycosaminoglycans rats protamines |
description |
PURPOSE: We reproduced a non-bacterial experimental model to assess bladder inflammation and urinary glycosaminoglycans (GAG) excretion and examined the effect of dimethyl sulfoxide (DMSO). MATERIALS AND METHODS: Female rats were instilled with either protamine sulfate (PS groups) or sterile saline (control groups). At different days after the procedure, 24 h urine and bladder samples were obtained. Urinary levels of hyaluronic acid (HA) and sulfated glycosaminoglycans (S-GAG) were determined. Also to evaluate the effect of DMSO animals were instilled with either 50% DMSO or saline 6 hours after PS instillation. To evaluate the effect of DMSO in healthy bladders, rats were instilled with 50% DMSO and controls with saline. RESULTS: In the PS groups, bladder inflammation was observed, with polymorphonuclear cells during the first days and lymphomononuclear in the last days. HA and S-GAG had 2 peaks of urinary excretion, at the 1st and 7th day after PS injection. DMSO significantly reduced bladder inflammation. In contrast, in healthy bladders, DMSO produced mild inflammation and an increase in urinary HA levels after 1 and 7 days and an increase of S-GAG level in 7 days. Animals instilled with PS and treated with DMSO had significantly reduced levels of urinary HA only at the 1st day. Urinary S-GAG/Cr levels were similar in all groups. CONCLUSIONS: Increased urinary levels of GAG were associated with bladder inflammation in a PS-induced cystitis model. DMSO significantly reduced the inflammatory process after urothelial injury. Conversely, this drug provoked mild inflammation in normal mucosa. DMSO treatment was shown to influence urinary HA excretion. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-08-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:38:40Z |
dc.date.available.fl_str_mv |
2015-06-14T13:38:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
International braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 4, p. 503-511, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/4512 http://dx.doi.org/10.1590/S1677-55382008000400013 |
dc.identifier.issn.none.fl_str_mv |
1677-5538 |
dc.identifier.file.none.fl_str_mv |
S1677-55382008000400013.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1677-55382008000400013 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1677-55382008000400013 |
dc.identifier.wos.none.fl_str_mv |
WOS:000207705100026 |
identifier_str_mv |
International braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 4, p. 503-511, 2008. 1677-5538 S1677-55382008000400013.pdf S1677-55382008000400013 10.1590/S1677-55382008000400013 WOS:000207705100026 |
url |
http://repositorio.unifesp.br/handle/11600/4512 http://dx.doi.org/10.1590/S1677-55382008000400013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
International braz j urol |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
503-511 |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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