Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1139/H2012-129 http://repositorio.unifesp.br/handle/11600/35527 |
Resumo: | The present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. the findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake. |
id |
UFSP_15050851965352a0b7d48aa89d47719f |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/35527 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat dietswim trainingrat liver carcinogenesishigh-fat dietpreneoplastic-neoplastic lesionsThe present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. the findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake.São Paulo State Univ, UNESP, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Inst Biosci, Postgrad Program Gen & Appl Biol, BR-18618970 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Sch Med, Dept Clin Med, BR-18618970 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Sch Med, Dept Pathol, BR-18618970 Botucatu, SP, BrazilUniv Fed Alfenas, UNIFAL, Fac Nutr, BR-37130000 Alfenas, MG, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, BrazilWeb of ScienceFundação para o Desenvolvimento da UNESP (FUNDUNESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FUNDUNESP: FUNDUNESP DFP- 0028610FAPESP: 2010/03056-9CNPq: 301585/2009-1Canadian Science Publishing, Nrc Research PressUniversidade de São Paulo (USP)Univ Fed AlfenasUniversidade Federal de São Paulo (UNIFESP)Aguiar e Silva, Marco AurelioVechetti-Junior, Ivan JoseNascimento, Andre Ferreira doFurtado, Kelly SilvaAzevedo, LucianaRibeiro, Daniel Araki [UNIFESP]Barbisan, Luis Fernando2016-01-24T14:28:03Z2016-01-24T14:28:03Z2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1101-1109http://dx.doi.org/10.1139/H2012-129Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 37, n. 6, p. 1101-1109, 2012.10.1139/H2012-1291715-5312http://repositorio.unifesp.br/handle/11600/35527WOS:000311483800010engApplied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolismeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:28:03Zoai:repositorio.unifesp.br/:11600/35527Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:28:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
title |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
spellingShingle |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet Aguiar e Silva, Marco Aurelio swim training rat liver carcinogenesis high-fat diet preneoplastic-neoplastic lesions |
title_short |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
title_full |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
title_fullStr |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
title_full_unstemmed |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
title_sort |
Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet |
author |
Aguiar e Silva, Marco Aurelio |
author_facet |
Aguiar e Silva, Marco Aurelio Vechetti-Junior, Ivan Jose Nascimento, Andre Ferreira do Furtado, Kelly Silva Azevedo, Luciana Ribeiro, Daniel Araki [UNIFESP] Barbisan, Luis Fernando |
author_role |
author |
author2 |
Vechetti-Junior, Ivan Jose Nascimento, Andre Ferreira do Furtado, Kelly Silva Azevedo, Luciana Ribeiro, Daniel Araki [UNIFESP] Barbisan, Luis Fernando |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Fed Alfenas Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Aguiar e Silva, Marco Aurelio Vechetti-Junior, Ivan Jose Nascimento, Andre Ferreira do Furtado, Kelly Silva Azevedo, Luciana Ribeiro, Daniel Araki [UNIFESP] Barbisan, Luis Fernando |
dc.subject.por.fl_str_mv |
swim training rat liver carcinogenesis high-fat diet preneoplastic-neoplastic lesions |
topic |
swim training rat liver carcinogenesis high-fat diet preneoplastic-neoplastic lesions |
description |
The present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. the findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 2016-01-24T14:28:03Z 2016-01-24T14:28:03Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1139/H2012-129 Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 37, n. 6, p. 1101-1109, 2012. 10.1139/H2012-129 1715-5312 http://repositorio.unifesp.br/handle/11600/35527 WOS:000311483800010 |
url |
http://dx.doi.org/10.1139/H2012-129 http://repositorio.unifesp.br/handle/11600/35527 |
identifier_str_mv |
Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 37, n. 6, p. 1101-1109, 2012. 10.1139/H2012-129 1715-5312 WOS:000311483800010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1101-1109 |
dc.publisher.none.fl_str_mv |
Canadian Science Publishing, Nrc Research Press |
publisher.none.fl_str_mv |
Canadian Science Publishing, Nrc Research Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268374264315904 |