Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet

Detalhes bibliográficos
Autor(a) principal: Aguiar e Silva, Marco Aurelio
Data de Publicação: 2012
Outros Autores: Vechetti-Junior, Ivan Jose, Nascimento, Andre Ferreira do, Furtado, Kelly Silva, Azevedo, Luciana, Ribeiro, Daniel Araki [UNIFESP], Barbisan, Luis Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1139/H2012-129
http://repositorio.unifesp.br/handle/11600/35527
Resumo: The present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. the findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake.
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spelling Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat dietswim trainingrat liver carcinogenesishigh-fat dietpreneoplastic-neoplastic lesionsThe present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. the findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake.São Paulo State Univ, UNESP, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Inst Biosci, Postgrad Program Gen & Appl Biol, BR-18618970 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Sch Med, Dept Clin Med, BR-18618970 Botucatu, SP, BrazilSão Paulo State Univ, UNESP, Sch Med, Dept Pathol, BR-18618970 Botucatu, SP, BrazilUniv Fed Alfenas, UNIFAL, Fac Nutr, BR-37130000 Alfenas, MG, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP, BrazilWeb of ScienceFundação para o Desenvolvimento da UNESP (FUNDUNESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FUNDUNESP: FUNDUNESP DFP- 0028610FAPESP: 2010/03056-9CNPq: 301585/2009-1Canadian Science Publishing, Nrc Research PressUniversidade de São Paulo (USP)Univ Fed AlfenasUniversidade Federal de São Paulo (UNIFESP)Aguiar e Silva, Marco AurelioVechetti-Junior, Ivan JoseNascimento, Andre Ferreira doFurtado, Kelly SilvaAzevedo, LucianaRibeiro, Daniel Araki [UNIFESP]Barbisan, Luis Fernando2016-01-24T14:28:03Z2016-01-24T14:28:03Z2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1101-1109http://dx.doi.org/10.1139/H2012-129Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 37, n. 6, p. 1101-1109, 2012.10.1139/H2012-1291715-5312http://repositorio.unifesp.br/handle/11600/35527WOS:000311483800010engApplied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolismeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:28:03Zoai:repositorio.unifesp.br/:11600/35527Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:28:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
title Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
spellingShingle Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
Aguiar e Silva, Marco Aurelio
swim training
rat liver carcinogenesis
high-fat diet
preneoplastic-neoplastic lesions
title_short Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
title_full Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
title_fullStr Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
title_full_unstemmed Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
title_sort Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet
author Aguiar e Silva, Marco Aurelio
author_facet Aguiar e Silva, Marco Aurelio
Vechetti-Junior, Ivan Jose
Nascimento, Andre Ferreira do
Furtado, Kelly Silva
Azevedo, Luciana
Ribeiro, Daniel Araki [UNIFESP]
Barbisan, Luis Fernando
author_role author
author2 Vechetti-Junior, Ivan Jose
Nascimento, Andre Ferreira do
Furtado, Kelly Silva
Azevedo, Luciana
Ribeiro, Daniel Araki [UNIFESP]
Barbisan, Luis Fernando
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Univ Fed Alfenas
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Aguiar e Silva, Marco Aurelio
Vechetti-Junior, Ivan Jose
Nascimento, Andre Ferreira do
Furtado, Kelly Silva
Azevedo, Luciana
Ribeiro, Daniel Araki [UNIFESP]
Barbisan, Luis Fernando
dc.subject.por.fl_str_mv swim training
rat liver carcinogenesis
high-fat diet
preneoplastic-neoplastic lesions
topic swim training
rat liver carcinogenesis
high-fat diet
preneoplastic-neoplastic lesions
description The present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. the findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
2016-01-24T14:28:03Z
2016-01-24T14:28:03Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1139/H2012-129
Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 37, n. 6, p. 1101-1109, 2012.
10.1139/H2012-129
1715-5312
http://repositorio.unifesp.br/handle/11600/35527
WOS:000311483800010
url http://dx.doi.org/10.1139/H2012-129
http://repositorio.unifesp.br/handle/11600/35527
identifier_str_mv Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 37, n. 6, p. 1101-1109, 2012.
10.1139/H2012-129
1715-5312
WOS:000311483800010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Physiology Nutrition and Metabolism-physiologie Appliquee Nutrition Et Metabolisme
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1101-1109
dc.publisher.none.fl_str_mv Canadian Science Publishing, Nrc Research Press
publisher.none.fl_str_mv Canadian Science Publishing, Nrc Research Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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