Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/56422 http://dx.doi.org/10.1590/1678-98652017000100002 |
Resumo: | Objective This study aimed to compare the intestinal absorption of iron and calcium between soy-based and cow's milk- based infant formulas in weanling rats. Methods Twenty male Wistar rats, twenty-one days old on the first day of weaning, were used in this experiment, divided in two Groups, one Group was fed soy protein-based infant formula the other, cow's milk protein-based infant formula. During the study period (ten consecutive days) the animals received food and water ad libitum. Hematocrit and hemoglobin were evaluated on the first, fifth, and tenth days by the Wintrobe and cyanomethemoglobin methods. Feces and urine were collected, beginning on the fifth day, for three consecutive days. On the tenth day, hepatic iron content was also analyzed. Hepatic iron as well as fecal and urinary iron and calcium analyses were performed using an atomic absorption spectrophotometer. At thirty-one days of age, the animals were anesthetized with ketamine and xylazine and sacrificed by exsanguination via the vena cava. Results The final concentration of hemoglobin in the group soy-based infant formula and milk-based infant formula were: 10.3 +/- 1.3g/dL and 10.9 +/- 1.0g/dL (p=0.310). The apparent absorption of iron and calcium, in that order, were: 73.4 +/- 10.2% and 70.2 +/- 9.5% |
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Correia Silva, Maisa de Lima [UNIFESP]Speridião, Patrícia da Graça Leite [UNIFESP]Marciano, Renata [UNIFESP]Amancio, Olga Maria Silverio [UNIFESP]Morais, Tania Beninga de [UNIFESP]Morais, Mauro Batista de [UNIFESP]2020-07-31T12:46:52Z2020-07-31T12:46:52Z2017Revista De Nutricao-Brazilian Journal Of Nutrition. Campinas, v. 30, n. 1, p. 13-22, 2017.1415-5273https://repositorio.unifesp.br/handle/11600/56422http://dx.doi.org/10.1590/1678-98652017000100002S1415-52732017000100013.pdfS1415-5273201700010001310.1590/1678-98652017000100002WOS:000396350700002Objective This study aimed to compare the intestinal absorption of iron and calcium between soy-based and cow's milk- based infant formulas in weanling rats. Methods Twenty male Wistar rats, twenty-one days old on the first day of weaning, were used in this experiment, divided in two Groups, one Group was fed soy protein-based infant formula the other, cow's milk protein-based infant formula. During the study period (ten consecutive days) the animals received food and water ad libitum. Hematocrit and hemoglobin were evaluated on the first, fifth, and tenth days by the Wintrobe and cyanomethemoglobin methods. Feces and urine were collected, beginning on the fifth day, for three consecutive days. On the tenth day, hepatic iron content was also analyzed. Hepatic iron as well as fecal and urinary iron and calcium analyses were performed using an atomic absorption spectrophotometer. At thirty-one days of age, the animals were anesthetized with ketamine and xylazine and sacrificed by exsanguination via the vena cava. Results The final concentration of hemoglobin in the group soy-based infant formula and milk-based infant formula were: 10.3 +/- 1.3g/dL and 10.9 +/- 1.0g/dL (p=0.310). The apparent absorption of iron and calcium, in that order, were: 73.4 +/- 10.2% and 70.2 +/- 9.5%97.2 +/- 0.7% and 97.6 +/- 1.0% (p=0.501p=0.290). The apparent calcium retention was: 88.4%+/- 2.2 and 88.6 +/- 2.6%(p=0.848). Hepatic iron content was: 522.0 +/- 121.1mg/g and 527.8 +/- 80.5mg/g (p=0.907). Conclusion Intestinal iron and calcium absorption from soy-based infant formula is similar to that from milk-based infant formula in weanling rats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Paulo, Escola Paulista Med, Dept Pediat, R Coronel Lisboa,826,Vila Clementino, BR-04020041 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Lab Bromotol & Microbiol Alimentos, BR-04020041 Sao Paulo, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Pediatria. R. Coronel Lisboa, 826, Vila Clementino, 04020-041, São Paulo, SP, BrasilUniversidade Federal de São Paulo, Escola Paulista de Medicina, Laboratório de Bromotologia e Microbiologia de Alimentos. São Paulo, SP, BrasilWeb of Science13-22engPontificia Universidade Catolica CampinasRevista De Nutricao-Brazilian Journal Of NutritionCalciumIntestinal absorptionIronMilk proteinsSoy proteinsIntestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pupsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCampinas301info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1415-52732017000100013.pdfapplication/pdf276643${dspace.ui.url}/bitstream/11600/56422/1/S1415-52732017000100013.pdfef353c63d8aa43fae576f715a5bd5e1bMD51open accessTEXTS1415-52732017000100013.pdf.txtS1415-52732017000100013.pdf.txtExtracted texttext/plain33895${dspace.ui.url}/bitstream/11600/56422/2/S1415-52732017000100013.pdf.txt15dc965b204e6279ec9d1392c52aa0ebMD52open accessTHUMBNAILS1415-52732017000100013.pdf.jpgS1415-52732017000100013.pdf.jpgIM Thumbnailimage/jpeg5341${dspace.ui.url}/bitstream/11600/56422/4/S1415-52732017000100013.pdf.jpg0878ba1afdaa4d50318f0ec1cb8bc628MD54open access11600/564222022-08-01 00:13:03.07open accessoai:repositorio.unifesp.br:11600/56422Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:11:53.723238Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
title |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
spellingShingle |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups Correia Silva, Maisa de Lima [UNIFESP] Calcium Intestinal absorption Iron Milk proteins Soy proteins |
title_short |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
title_full |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
title_fullStr |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
title_full_unstemmed |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
title_sort |
Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups |
author |
Correia Silva, Maisa de Lima [UNIFESP] |
author_facet |
Correia Silva, Maisa de Lima [UNIFESP] Speridião, Patrícia da Graça Leite [UNIFESP] Marciano, Renata [UNIFESP] Amancio, Olga Maria Silverio [UNIFESP] Morais, Tania Beninga de [UNIFESP] Morais, Mauro Batista de [UNIFESP] |
author_role |
author |
author2 |
Speridião, Patrícia da Graça Leite [UNIFESP] Marciano, Renata [UNIFESP] Amancio, Olga Maria Silverio [UNIFESP] Morais, Tania Beninga de [UNIFESP] Morais, Mauro Batista de [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Correia Silva, Maisa de Lima [UNIFESP] Speridião, Patrícia da Graça Leite [UNIFESP] Marciano, Renata [UNIFESP] Amancio, Olga Maria Silverio [UNIFESP] Morais, Tania Beninga de [UNIFESP] Morais, Mauro Batista de [UNIFESP] |
dc.subject.eng.fl_str_mv |
Calcium Intestinal absorption Iron Milk proteins Soy proteins |
topic |
Calcium Intestinal absorption Iron Milk proteins Soy proteins |
description |
Objective This study aimed to compare the intestinal absorption of iron and calcium between soy-based and cow's milk- based infant formulas in weanling rats. Methods Twenty male Wistar rats, twenty-one days old on the first day of weaning, were used in this experiment, divided in two Groups, one Group was fed soy protein-based infant formula the other, cow's milk protein-based infant formula. During the study period (ten consecutive days) the animals received food and water ad libitum. Hematocrit and hemoglobin were evaluated on the first, fifth, and tenth days by the Wintrobe and cyanomethemoglobin methods. Feces and urine were collected, beginning on the fifth day, for three consecutive days. On the tenth day, hepatic iron content was also analyzed. Hepatic iron as well as fecal and urinary iron and calcium analyses were performed using an atomic absorption spectrophotometer. At thirty-one days of age, the animals were anesthetized with ketamine and xylazine and sacrificed by exsanguination via the vena cava. Results The final concentration of hemoglobin in the group soy-based infant formula and milk-based infant formula were: 10.3 +/- 1.3g/dL and 10.9 +/- 1.0g/dL (p=0.310). The apparent absorption of iron and calcium, in that order, were: 73.4 +/- 10.2% and 70.2 +/- 9.5% |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-31T12:46:52Z |
dc.date.available.fl_str_mv |
2020-07-31T12:46:52Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Revista De Nutricao-Brazilian Journal Of Nutrition. Campinas, v. 30, n. 1, p. 13-22, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/56422 http://dx.doi.org/10.1590/1678-98652017000100002 |
dc.identifier.issn.none.fl_str_mv |
1415-5273 |
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S1415-52732017000100013.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1415-52732017000100013 |
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10.1590/1678-98652017000100002 |
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WOS:000396350700002 |
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Revista De Nutricao-Brazilian Journal Of Nutrition. Campinas, v. 30, n. 1, p. 13-22, 2017. 1415-5273 S1415-52732017000100013.pdf S1415-52732017000100013 10.1590/1678-98652017000100002 WOS:000396350700002 |
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https://repositorio.unifesp.br/handle/11600/56422 http://dx.doi.org/10.1590/1678-98652017000100002 |
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eng |
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Revista De Nutricao-Brazilian Journal Of Nutrition |
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openAccess |
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13-22 |
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Campinas |
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Pontificia Universidade Catolica Campinas |
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Pontificia Universidade Catolica Campinas |
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