Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/42477 http://www.ajtmh.org/content/68/6/661.abstract |
Resumo: | We describe the pathologic alterations of the central nervous system (CNS) observed in experimental tegumentary leishmaniasis in BALB/c and Swiss mice. The mice were subcutaneously infected with 10(4) amastigotes of Leishmania (Leishmania) amazonensis. Animals were killed and brains were removed for histologic and immunocytochemical studies. Histologic examination showed that 66.6% of infected mice had a discrete hyperemia and inflammatory infiltrate in the meninges, composed of mononuclear cells and neutrophils with no detectable parasites. However, parasitized macrophages were detected in the cerebral parenchyma, as well as mast cells, lymphocytes, and polymorphonuclear cells. Necrosis in the cerebral parenchyma was also observed. Confocal fluorescence microscopy showed that CD8(+) T lymphocytes are the major component of the inflammatory infiltrate in the CNS. In addition to these cells, CD4(+), CD11b, and dendritic cells are present, in small numbers, in the inflammatory processes of the CNS. Thus, L. amazonensis is able to cross the blood-brain barrier and cause significant pathologic changes in the CNS. |
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Abreu-Silva, Ana LuciaCalabrese, Katia da SilvaTedesco, Roberto Carlos [UNIFESP]Mortara, Renato Arruda [UNIFESP]Costa, SCG deUniv Estadual MaranhaoFiocruz MSUniversidade Federal de São Paulo (UNIFESP)2018-06-15T13:46:00Z2018-06-15T13:46:00Z2003-06-01American Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 68, n. 6, p. 661-665, 2003.0002-9637http://repositorio.unifesp.br/11600/42477http://www.ajtmh.org/content/68/6/661.abstractWOS:000184075500010We describe the pathologic alterations of the central nervous system (CNS) observed in experimental tegumentary leishmaniasis in BALB/c and Swiss mice. The mice were subcutaneously infected with 10(4) amastigotes of Leishmania (Leishmania) amazonensis. Animals were killed and brains were removed for histologic and immunocytochemical studies. Histologic examination showed that 66.6% of infected mice had a discrete hyperemia and inflammatory infiltrate in the meninges, composed of mononuclear cells and neutrophils with no detectable parasites. However, parasitized macrophages were detected in the cerebral parenchyma, as well as mast cells, lymphocytes, and polymorphonuclear cells. Necrosis in the cerebral parenchyma was also observed. Confocal fluorescence microscopy showed that CD8(+) T lymphocytes are the major component of the inflammatory infiltrate in the CNS. In addition to these cells, CD4(+), CD11b, and dendritic cells are present, in small numbers, in the inflammatory processes of the CNS. Thus, L. amazonensis is able to cross the blood-brain barrier and cause significant pathologic changes in the CNS.Univ Estadual Maranhao, Dept Patol, Maranhao, BrazilFiocruz MS, Inst Oswaldo Cruz, Lab Imunomodulacao, Dept Protozool, BR-21045900 Rio De Janeiro, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Morfol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Morfol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilWeb of Science661-665engAmer Soc Trop Med & HygieneAmerican Journal Of Tropical Medicine And HygieneCentral nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/424772022-02-08 11:53:19.304metadata only accessoai:repositorio.unifesp.br:11600/42477Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T14:53:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
title |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
spellingShingle |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis Abreu-Silva, Ana Lucia |
title_short |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
title_full |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
title_fullStr |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
title_full_unstemmed |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
title_sort |
Central nervous system involvement in experimental infection with Leishmania (Leishmania) amazonensis |
author |
Abreu-Silva, Ana Lucia |
author_facet |
Abreu-Silva, Ana Lucia Calabrese, Katia da Silva Tedesco, Roberto Carlos [UNIFESP] Mortara, Renato Arruda [UNIFESP] Costa, SCG de |
author_role |
author |
author2 |
Calabrese, Katia da Silva Tedesco, Roberto Carlos [UNIFESP] Mortara, Renato Arruda [UNIFESP] Costa, SCG de |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Estadual Maranhao Fiocruz MS Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Abreu-Silva, Ana Lucia Calabrese, Katia da Silva Tedesco, Roberto Carlos [UNIFESP] Mortara, Renato Arruda [UNIFESP] Costa, SCG de |
description |
We describe the pathologic alterations of the central nervous system (CNS) observed in experimental tegumentary leishmaniasis in BALB/c and Swiss mice. The mice were subcutaneously infected with 10(4) amastigotes of Leishmania (Leishmania) amazonensis. Animals were killed and brains were removed for histologic and immunocytochemical studies. Histologic examination showed that 66.6% of infected mice had a discrete hyperemia and inflammatory infiltrate in the meninges, composed of mononuclear cells and neutrophils with no detectable parasites. However, parasitized macrophages were detected in the cerebral parenchyma, as well as mast cells, lymphocytes, and polymorphonuclear cells. Necrosis in the cerebral parenchyma was also observed. Confocal fluorescence microscopy showed that CD8(+) T lymphocytes are the major component of the inflammatory infiltrate in the CNS. In addition to these cells, CD4(+), CD11b, and dendritic cells are present, in small numbers, in the inflammatory processes of the CNS. Thus, L. amazonensis is able to cross the blood-brain barrier and cause significant pathologic changes in the CNS. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003-06-01 |
dc.date.accessioned.fl_str_mv |
2018-06-15T13:46:00Z |
dc.date.available.fl_str_mv |
2018-06-15T13:46:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
American Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 68, n. 6, p. 661-665, 2003. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/42477 http://www.ajtmh.org/content/68/6/661.abstract |
dc.identifier.issn.none.fl_str_mv |
0002-9637 |
dc.identifier.wos.none.fl_str_mv |
WOS:000184075500010 |
identifier_str_mv |
American Journal Of Tropical Medicine And Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 68, n. 6, p. 661-665, 2003. 0002-9637 WOS:000184075500010 |
url |
http://repositorio.unifesp.br/11600/42477 http://www.ajtmh.org/content/68/6/661.abstract |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
American Journal Of Tropical Medicine And Hygiene |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
661-665 |
dc.publisher.none.fl_str_mv |
Amer Soc Trop Med & Hygiene |
publisher.none.fl_str_mv |
Amer Soc Trop Med & Hygiene |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764177531469824 |