Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1111/bju.13539 https://repositorio.unifesp.br/handle/11600/56856 |
Resumo: | Objective To evaluate the effect of smoking on sperm functional quality and seminal plasma proteomic profile. Patients and Methods Sperm functional tests were performed in 20 non-smoking men with normal semen quality, according to the World Health Organization (2010) and in 20 smoking patients. These included: evaluation of DNA fragmentation by alkaline Comet assay |
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Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokersacrosomeDNA damagemitochondriaproteomicsseminal plasmaObjective To evaluate the effect of smoking on sperm functional quality and seminal plasma proteomic profile. Patients and Methods Sperm functional tests were performed in 20 non-smoking men with normal semen quality, according to the World Health Organization (2010) and in 20 smoking patients. These included: evaluation of DNA fragmentation by alkaline Comet assayanalysis of mitochondrial activity using DAB stainingand acrosomal integrity evaluation by PNA binding. The remaining semen was centrifuged and seminal plasma was used for proteomic analysis (liquid chromatographytandem mass spectrometry). The quantified proteins were used for Venn diagram construction in Cytoscape 3.2.1 software, using the PINA4MS plug-in. Then, differentially expressed proteins were used for functional enrichment analysis of Gene Ontology categories, Kyoto Encyclopedia of Genes and Genomes and Reactome, using Cytoscape software and the ClueGO 2.2.0 plug-in. Results Smokers had a higher percentage of sperm DNA damage (Comet classes III and IVP < 0.01), partially and fully inactive mitochondria (DAB classes III and IVP = 0.001 and P = 0.006, respectively) and non-intact acrosomes (P < 0.01) when compared with the control group. With respect to proteomic analysis, 422 proteins were identified and quantified, of which one protein was absent, 27 proteins were under-represented and six proteins were over-represented in smokers. Functional enrichment analysis showed the enrichment of antigen processing and presentation, positive regulation of prostaglandin secretion involved in immune response, protein kinase A signalling and arachidonic acid secretion, complement activation, regulation of the cytokinemediated signalling pathway and regulation of acute inflammatory response in the study group (smokers). Conclusion In conclusion, cigarette smoking was associated with an inflammatory state in the accessory glands and in the testis, as shown by enriched proteomic pathways. This state causes an alteration in sperm functional quality, which is characterized by decreased acrosome integrity and mitochondrial activity, as well as by increased nuclear DNA fragmentation.Univ Fed Sao Paulo, Div Urol, Human Reprod Sect, Dept Surg, Sao Paulo, BrazilFleury Grp, Sao Paulo, BrazilHosp Sao Paulo, Sao Paulo, BrazilDepartment of Surgery, Division of Urology, Human Reproduction Section, Universidade Federal de São Paulo (UNIFESP), Sao Paulo, BrazilHospital Sao Paulo, Sao Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fleury Projects Funding (Fleury Group)CNPq: 160857/2012-0CNPq: 306616/2013-0Wiley2020-07-31T12:47:29Z2020-07-31T12:47:29Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion814-822http://dx.doi.org/10.1111/bju.13539Bju International. Hoboken, v. 118, n. 5, p. 814-822, 2016.10.1111/bju.135391464-4096https://repositorio.unifesp.br/handle/11600/56856WOS:000386468600025engBju InternationalHobokeninfo:eu-repo/semantics/openAccessAntoniassi, Mariana Pereira [UNIFESP]Intasqui, Paula [UNIFESP]Camargo, Mariana [UNIFESP]Zylbersztejn, Daniel Suslik [UNIFESP]Carvalho, Valdemir MelechcoCardozo, Karina H. M.Bertolla, Ricardo Pimenta [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-29T15:59:33Zoai:repositorio.unifesp.br/:11600/56856Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-29T15:59:33Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| title |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| spellingShingle |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers Antoniassi, Mariana Pereira [UNIFESP] acrosome DNA damage mitochondria proteomics seminal plasma |
| title_short |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| title_full |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| title_fullStr |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| title_full_unstemmed |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| title_sort |
Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers |
| author |
Antoniassi, Mariana Pereira [UNIFESP] |
| author_facet |
Antoniassi, Mariana Pereira [UNIFESP] Intasqui, Paula [UNIFESP] Camargo, Mariana [UNIFESP] Zylbersztejn, Daniel Suslik [UNIFESP] Carvalho, Valdemir Melechco Cardozo, Karina H. M. Bertolla, Ricardo Pimenta [UNIFESP] |
| author_role |
author |
| author2 |
Intasqui, Paula [UNIFESP] Camargo, Mariana [UNIFESP] Zylbersztejn, Daniel Suslik [UNIFESP] Carvalho, Valdemir Melechco Cardozo, Karina H. M. Bertolla, Ricardo Pimenta [UNIFESP] |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Antoniassi, Mariana Pereira [UNIFESP] Intasqui, Paula [UNIFESP] Camargo, Mariana [UNIFESP] Zylbersztejn, Daniel Suslik [UNIFESP] Carvalho, Valdemir Melechco Cardozo, Karina H. M. Bertolla, Ricardo Pimenta [UNIFESP] |
| dc.subject.por.fl_str_mv |
acrosome DNA damage mitochondria proteomics seminal plasma |
| topic |
acrosome DNA damage mitochondria proteomics seminal plasma |
| description |
Objective To evaluate the effect of smoking on sperm functional quality and seminal plasma proteomic profile. Patients and Methods Sperm functional tests were performed in 20 non-smoking men with normal semen quality, according to the World Health Organization (2010) and in 20 smoking patients. These included: evaluation of DNA fragmentation by alkaline Comet assay |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2020-07-31T12:47:29Z 2020-07-31T12:47:29Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/bju.13539 Bju International. Hoboken, v. 118, n. 5, p. 814-822, 2016. 10.1111/bju.13539 1464-4096 https://repositorio.unifesp.br/handle/11600/56856 WOS:000386468600025 |
| url |
http://dx.doi.org/10.1111/bju.13539 https://repositorio.unifesp.br/handle/11600/56856 |
| identifier_str_mv |
Bju International. Hoboken, v. 118, n. 5, p. 814-822, 2016. 10.1111/bju.13539 1464-4096 WOS:000386468600025 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Bju International |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
814-822 |
| dc.coverage.none.fl_str_mv |
Hoboken |
| dc.publisher.none.fl_str_mv |
Wiley |
| publisher.none.fl_str_mv |
Wiley |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268365404897280 |