A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2003 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0074-02762003000100016 http://repositorio.unifesp.br/handle/11600/1640 |
Resumo: | We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions. |
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A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruziTrypanosoma cruziinterspersed repetitive DNAtranscriptionretroelement insertionsubtelomeric regionWe report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions.Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaNorthwestern University Medical School Departments of Pathology and Microbiology-ImmunologyUniversidad Central de Venezuela Instituto de Biologia Experimental Laboratorio de Genetica MolecularUniversidad de Antofagasta Departamento de Tecnologia MédicaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de São Paulo (UNIFESP)Northwestern University Medical School Departments of Pathology and Microbiology-ImmunologyUniversidad Central de Venezuela Instituto de Biologia Experimental Laboratorio de Genetica MolecularUniversidad de Antofagasta Departamento de Tecnologia MédicaVerbisck, Newton Valério [UNIFESP]Santos, Márcia Regina Machado dos [UNIFESP]Engman, David MAngel Chiurillo, MiguelRamirez, José LuisAraya, Jorge EnriqueMortara, Renato Arruda [UNIFESP]Franco da Silveira, José [UNIFESP]2015-06-14T13:29:55Z2015-06-14T13:29:55Z2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion129-133application/pdfhttp://dx.doi.org/10.1590/S0074-02762003000100016Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 1, p. 129-133, 2003.10.1590/S0074-02762003000100016S0074-02762003000100016.pdf0074-0276S0074-02762003000100016http://repositorio.unifesp.br/handle/11600/1640engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T15:30:34Zoai:repositorio.unifesp.br/:11600/1640Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T15:30:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| title |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| spellingShingle |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi Verbisck, Newton Valério [UNIFESP] Trypanosoma cruzi interspersed repetitive DNA transcription retroelement insertion subtelomeric region |
| title_short |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| title_full |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| title_fullStr |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| title_full_unstemmed |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| title_sort |
A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi |
| author |
Verbisck, Newton Valério [UNIFESP] |
| author_facet |
Verbisck, Newton Valério [UNIFESP] Santos, Márcia Regina Machado dos [UNIFESP] Engman, David M Angel Chiurillo, Miguel Ramirez, José Luis Araya, Jorge Enrique Mortara, Renato Arruda [UNIFESP] Franco da Silveira, José [UNIFESP] |
| author_role |
author |
| author2 |
Santos, Márcia Regina Machado dos [UNIFESP] Engman, David M Angel Chiurillo, Miguel Ramirez, José Luis Araya, Jorge Enrique Mortara, Renato Arruda [UNIFESP] Franco da Silveira, José [UNIFESP] |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Northwestern University Medical School Departments of Pathology and Microbiology-Immunology Universidad Central de Venezuela Instituto de Biologia Experimental Laboratorio de Genetica Molecular Universidad de Antofagasta Departamento de Tecnologia Médica |
| dc.contributor.author.fl_str_mv |
Verbisck, Newton Valério [UNIFESP] Santos, Márcia Regina Machado dos [UNIFESP] Engman, David M Angel Chiurillo, Miguel Ramirez, José Luis Araya, Jorge Enrique Mortara, Renato Arruda [UNIFESP] Franco da Silveira, José [UNIFESP] |
| dc.subject.por.fl_str_mv |
Trypanosoma cruzi interspersed repetitive DNA transcription retroelement insertion subtelomeric region |
| topic |
Trypanosoma cruzi interspersed repetitive DNA transcription retroelement insertion subtelomeric region |
| description |
We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions. |
| publishDate |
2003 |
| dc.date.none.fl_str_mv |
2003-01-01 2015-06-14T13:29:55Z 2015-06-14T13:29:55Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762003000100016 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 1, p. 129-133, 2003. 10.1590/S0074-02762003000100016 S0074-02762003000100016.pdf 0074-0276 S0074-02762003000100016 http://repositorio.unifesp.br/handle/11600/1640 |
| url |
http://dx.doi.org/10.1590/S0074-02762003000100016 http://repositorio.unifesp.br/handle/11600/1640 |
| identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 1, p. 129-133, 2003. 10.1590/S0074-02762003000100016 S0074-02762003000100016.pdf 0074-0276 S0074-02762003000100016 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
129-133 application/pdf |
| dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
| institution |
UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268336534454272 |