Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Giret, Maria Teresa M.
Data de Publicação: 2011
Outros Autores: Miraglia, Joao Luiz, Sucupira, Maria Cecilia Araripe [UNIFESP], Nishiya, Anna, Levi, Jose Eduardo, Diaz, Ricardo Sobhie [UNIFESP], Sabino, Ester Cerdeira, Kallas, Esper Georges [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0018407
http://repositorio.unifesp.br/handle/11600/33630
Resumo: Background: the results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1) due to similar transmission routes of both viruses. the aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population.Methodology/Principal Findings: the study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in São Paulo, Brazil. the presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. the overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29) in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+ T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b.Conclusion/Significance: GBV-C infection is common in recently HIV -1 infected patients in São Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year.
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spelling Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, BrazilBackground: the results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1) due to similar transmission routes of both viruses. the aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population.Methodology/Principal Findings: the study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in São Paulo, Brazil. the presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. the overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29) in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+ T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b.Conclusion/Significance: GBV-C infection is common in recently HIV -1 infected patients in São Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year.Univ São Paulo, Div Clin Immunol & Allergy, São Paulo, BrazilUniversidade Federal de São Paulo, Div Infect Dis, São Paulo, BrazilSão Paulo Blood Bank, São Paulo, BrazilUniv São Paulo, Inst Trop Med, São Paulo, BrazilUniversidade Federal de São Paulo, Div Infect Dis, São Paulo, BrazilWeb of ScienceMinistry of HealthSão Paulo City Health DepartmentFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Ministry of Health: 914/BRA/3014-UNESCO/KallasSão Paulo City Health Department: 2004-0.168.922-7/KallasFAPESP: 04/15856-9/Diaz, Sabino KallasFAPESP: 05/01072-9/LeviPublic Library ScienceUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)São Paulo Blood BankGiret, Maria Teresa M.Miraglia, Joao LuizSucupira, Maria Cecilia Araripe [UNIFESP]Nishiya, AnnaLevi, Jose EduardoDiaz, Ricardo Sobhie [UNIFESP]Sabino, Ester CerdeiraKallas, Esper Georges [UNIFESP]2016-01-24T14:06:24Z2016-01-24T14:06:24Z2011-04-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9application/pdfhttp://dx.doi.org/10.1371/journal.pone.0018407Plos One. San Francisco: Public Library Science, v. 6, n. 4, 9 p., 2011.10.1371/journal.pone.0018407WOS000289191300021.pdf1932-6203http://repositorio.unifesp.br/handle/11600/33630WOS:000289191300021engPlos Oneinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T06:04:47Zoai:repositorio.unifesp.br/:11600/33630Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T06:04:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
title Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
spellingShingle Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
Giret, Maria Teresa M.
title_short Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
title_full Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
title_fullStr Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
title_full_unstemmed Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
title_sort Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in São Paulo, Brazil
author Giret, Maria Teresa M.
author_facet Giret, Maria Teresa M.
Miraglia, Joao Luiz
Sucupira, Maria Cecilia Araripe [UNIFESP]
Nishiya, Anna
Levi, Jose Eduardo
Diaz, Ricardo Sobhie [UNIFESP]
Sabino, Ester Cerdeira
Kallas, Esper Georges [UNIFESP]
author_role author
author2 Miraglia, Joao Luiz
Sucupira, Maria Cecilia Araripe [UNIFESP]
Nishiya, Anna
Levi, Jose Eduardo
Diaz, Ricardo Sobhie [UNIFESP]
Sabino, Ester Cerdeira
Kallas, Esper Georges [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
São Paulo Blood Bank
dc.contributor.author.fl_str_mv Giret, Maria Teresa M.
Miraglia, Joao Luiz
Sucupira, Maria Cecilia Araripe [UNIFESP]
Nishiya, Anna
Levi, Jose Eduardo
Diaz, Ricardo Sobhie [UNIFESP]
Sabino, Ester Cerdeira
Kallas, Esper Georges [UNIFESP]
description Background: the results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1) due to similar transmission routes of both viruses. the aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population.Methodology/Principal Findings: the study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in São Paulo, Brazil. the presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. the overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29) in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+ T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b.Conclusion/Significance: GBV-C infection is common in recently HIV -1 infected patients in São Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-05
2016-01-24T14:06:24Z
2016-01-24T14:06:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0018407
Plos One. San Francisco: Public Library Science, v. 6, n. 4, 9 p., 2011.
10.1371/journal.pone.0018407
WOS000289191300021.pdf
1932-6203
http://repositorio.unifesp.br/handle/11600/33630
WOS:000289191300021
url http://dx.doi.org/10.1371/journal.pone.0018407
http://repositorio.unifesp.br/handle/11600/33630
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 6, n. 4, 9 p., 2011.
10.1371/journal.pone.0018407
WOS000289191300021.pdf
1932-6203
WOS:000289191300021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos One
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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