Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)

Detalhes bibliográficos
Autor(a) principal: Caires-Junior, Luiz Carlos
Data de Publicação: 2018
Outros Autores: Goulart, Ernesto, Melo, Uira Souto, Araujo, Bruno Henrique Silva [UNIFESP], Alvizi, Lucas, Soares-Schanoski, Alessandra, Oliveira, Danyllo Felipe de, Kobayashi, Gerson Shigeru, Griesi-Oliveira, Karina, Musso, Camila Manso, Amaral, Murilo Sena, Silva, Lucas Ferreira da, Astray, Renato Mancini, Suarez-Patino, Sandra Fernanda, Ventini, Daniella Cristina, Silva, Sergio Gomes da, Yamamoto, Guilherme Lopes, Ezquina, Suzana, Naslavsky, Michel Satya, Telles-Silva, Kayque Alves, Weinmann, Karina, van der Linden, Vanessa, van der Linden, Helio, Oliveira, Joao Ricardo Mendes de, Arrais, Nivia Maria Rodrigues, Melo, Adriana, Figueiredo, Thalita, Santos, Silvana, Meira, Joanna Goes Castro, Passos, Saulo Duarte, Almeida, Roque Pacheco de, Bispo, Ana Jovina Barreto, Cavalheiro, Esper Abrão [UNIFESP], Kalil, Jorge, Cunha-Neto, Edecio, Nakaya, Helder, Andreata-Santos, Robert, Ferreira, Luis Carlos de Souza, Verjovski-Almeida, Sergio, Ho, Paulo Lee, Passos-Bueno, Maria Rita, Zatz, Mayana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1038/s41467-017-02790-9
https://repositorio.unifesp.br/handle/11600/55823
Resumo: Congenital Zika syndrome (CZS) causes early brain development impairment by affecting neural progenitor cells (NPCs). Here, we analyze NPCs from three pairs of dizygotic twins discordant for CZS. We compare by RNA-Seq the NPCs derived from CZS-affected and CZS unaffected twins. Prior to Zika virus (ZIKV) infection the NPCs from CZS babies show a significantly different gene expression signature of mTOR and Wnt pathway regulators, key to a neurodevelopmental program. Following ZIKV in vitro infection, cells from affected individuals have significantly higher ZIKV replication and reduced cell growth. Whole-exome analysis in 18 affected CZS babies as compared to 5 unaffected twins and 609 controls excludes a monogenic model to explain resistance or increased susceptibility to CZS development. Overall, our results indicate that CZS is not a stochastic event and depends on NPC intrinsic susceptibility, possibly related to oligogenic and/or epigenetic mechanisms.
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spelling Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)Congenital Zika syndrome (CZS) causes early brain development impairment by affecting neural progenitor cells (NPCs). Here, we analyze NPCs from three pairs of dizygotic twins discordant for CZS. We compare by RNA-Seq the NPCs derived from CZS-affected and CZS unaffected twins. Prior to Zika virus (ZIKV) infection the NPCs from CZS babies show a significantly different gene expression signature of mTOR and Wnt pathway regulators, key to a neurodevelopmental program. Following ZIKV in vitro infection, cells from affected individuals have significantly higher ZIKV replication and reduced cell growth. Whole-exome analysis in 18 affected CZS babies as compared to 5 unaffected twins and 609 controls excludes a monogenic model to explain resistance or increased susceptibility to CZS development. Overall, our results indicate that CZS is not a stochastic event and depends on NPC intrinsic susceptibility, possibly related to oligogenic and/or epigenetic mechanisms.Univ Sao Paulo, Biosci Inst, Human Genome & Stem Cell Res Ctr, Dept Genet & Evolutionary Biol, BR-05508900 Sao Paulo, SP, BrazilBrazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP, BrazilFed Univ Sao Paulo UNIFESP, EPM, Dept Neurol & Neurosurg, Neurosci Lab, BR-04039002 Sao Paulo, SP, BrazilButantan Inst, BR-05503900 Sao Paulo, SP, BrazilAlbert Einstein Hosp, BR-05652900 Sao Paulo, SP, BrazilUniv Sao Paulo, Inst Chem, Dept Biochem, BR-05508900 Sao Paulo, SP, BrazilUniv Mogi das Cruzes, BR-08780911 Mogi Das Cruzes, SP, BrazilAACD, BR-50080810 Recife, PE, BrazilRehabil Ctr Dr Henrique Santillo, BR-74653230 Goiania, Go, BrazilFed Univ Pernambuco UFPE, Neuropsychiat Dept, BR-50670901 Recife, PE, BrazilFed Univ Pernambuco UFPE, KeizoAsami Lab, BR-50670901 Recife, PE, BrazilFed Univ Rio Grande do Norte UFRN, Dept Pediat, BR-59010180 Natal, RN, BrazilISEA, BR-58400220 Campina Grande, PB, BrazilParaiba State Univ UEPB, Dept Biol, BR-58429500 Campina Grande, PB, BrazilFed Univ Bahia UFBA, BR-40170115 Salvador, BA, BrazilMed Sch Jundiai, Infect Pediat Lab, BR-40170115 Jundiai, SP, BrazilFed Univ Sergipe UFS, Div Immunol, BR-49100000 Aracaju, SP, BrazilFed Univ Sergipe UFS, Mol Biol Lab, BR-49100000 Aracaju, SP, BrazilUniv Sao Paulo, Fac Med, Heart Inst, BR-05403900 Sao Paulo, SP, BrazilUniv Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo, SP, BrazilUniv Sao Paulo, Inst Biomed Sci, Dept Microbiol, Vaccine Dev Lab, BR-05508900 Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, EPM, Dept Neurol & Neurosurg, Neurosci Lab, BR-04039002 Sao Paulo, SP, BrazilWeb of ScienceCAPESDECIT-MSNature Publishing Group2020-07-20T16:31:15Z2020-07-20T16:31:15Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.1038/s41467-017-02790-9Nature Communications. London, v. 9, 2018.10.1038/s41467-017-02790-9WOS000427248800003a.pdf2041-1723https://repositorio.unifesp.br/handle/11600/55823WOS:000427248800003aengNature CommunicationsLondoninfo:eu-repo/semantics/openAccessCaires-Junior, Luiz CarlosGoulart, ErnestoMelo, Uira SoutoAraujo, Bruno Henrique Silva [UNIFESP]Alvizi, LucasSoares-Schanoski, AlessandraOliveira, Danyllo Felipe deKobayashi, Gerson ShigeruGriesi-Oliveira, KarinaMusso, Camila MansoAmaral, Murilo SenaSilva, Lucas Ferreira daAstray, Renato ManciniSuarez-Patino, Sandra FernandaVentini, Daniella CristinaSilva, Sergio Gomes daYamamoto, Guilherme LopesEzquina, SuzanaNaslavsky, Michel SatyaTelles-Silva, Kayque AlvesWeinmann, Karinavan der Linden, Vanessavan der Linden, HelioOliveira, Joao Ricardo Mendes deArrais, Nivia Maria RodriguesMelo, AdrianaFigueiredo, ThalitaSantos, SilvanaMeira, Joanna Goes CastroPassos, Saulo DuarteAlmeida, Roque Pacheco deBispo, Ana Jovina BarretoCavalheiro, Esper Abrão [UNIFESP]Kalil, JorgeCunha-Neto, EdecioNakaya, HelderAndreata-Santos, RobertFerreira, Luis Carlos de SouzaVerjovski-Almeida, SergioHo, Paulo LeePassos-Bueno, Maria RitaZatz, Mayanareponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T08:35:50Zoai:repositorio.unifesp.br/:11600/55823Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T08:35:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
title Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
spellingShingle Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
Caires-Junior, Luiz Carlos
title_short Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
title_full Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
title_fullStr Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
title_full_unstemmed Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
title_sort Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells (vol 9, 2018)
author Caires-Junior, Luiz Carlos
author_facet Caires-Junior, Luiz Carlos
Goulart, Ernesto
Melo, Uira Souto
Araujo, Bruno Henrique Silva [UNIFESP]
Alvizi, Lucas
Soares-Schanoski, Alessandra
Oliveira, Danyllo Felipe de
Kobayashi, Gerson Shigeru
Griesi-Oliveira, Karina
Musso, Camila Manso
Amaral, Murilo Sena
Silva, Lucas Ferreira da
Astray, Renato Mancini
Suarez-Patino, Sandra Fernanda
Ventini, Daniella Cristina
Silva, Sergio Gomes da
Yamamoto, Guilherme Lopes
Ezquina, Suzana
Naslavsky, Michel Satya
Telles-Silva, Kayque Alves
Weinmann, Karina
van der Linden, Vanessa
van der Linden, Helio
Oliveira, Joao Ricardo Mendes de
Arrais, Nivia Maria Rodrigues
Melo, Adriana
Figueiredo, Thalita
Santos, Silvana
Meira, Joanna Goes Castro
Passos, Saulo Duarte
Almeida, Roque Pacheco de
Bispo, Ana Jovina Barreto
Cavalheiro, Esper Abrão [UNIFESP]
Kalil, Jorge
Cunha-Neto, Edecio
Nakaya, Helder
Andreata-Santos, Robert
Ferreira, Luis Carlos de Souza
Verjovski-Almeida, Sergio
Ho, Paulo Lee
Passos-Bueno, Maria Rita
Zatz, Mayana
author_role author
author2 Goulart, Ernesto
Melo, Uira Souto
Araujo, Bruno Henrique Silva [UNIFESP]
Alvizi, Lucas
Soares-Schanoski, Alessandra
Oliveira, Danyllo Felipe de
Kobayashi, Gerson Shigeru
Griesi-Oliveira, Karina
Musso, Camila Manso
Amaral, Murilo Sena
Silva, Lucas Ferreira da
Astray, Renato Mancini
Suarez-Patino, Sandra Fernanda
Ventini, Daniella Cristina
Silva, Sergio Gomes da
Yamamoto, Guilherme Lopes
Ezquina, Suzana
Naslavsky, Michel Satya
Telles-Silva, Kayque Alves
Weinmann, Karina
van der Linden, Vanessa
van der Linden, Helio
Oliveira, Joao Ricardo Mendes de
Arrais, Nivia Maria Rodrigues
Melo, Adriana
Figueiredo, Thalita
Santos, Silvana
Meira, Joanna Goes Castro
Passos, Saulo Duarte
Almeida, Roque Pacheco de
Bispo, Ana Jovina Barreto
Cavalheiro, Esper Abrão [UNIFESP]
Kalil, Jorge
Cunha-Neto, Edecio
Nakaya, Helder
Andreata-Santos, Robert
Ferreira, Luis Carlos de Souza
Verjovski-Almeida, Sergio
Ho, Paulo Lee
Passos-Bueno, Maria Rita
Zatz, Mayana
author2_role author
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author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
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dc.contributor.author.fl_str_mv Caires-Junior, Luiz Carlos
Goulart, Ernesto
Melo, Uira Souto
Araujo, Bruno Henrique Silva [UNIFESP]
Alvizi, Lucas
Soares-Schanoski, Alessandra
Oliveira, Danyllo Felipe de
Kobayashi, Gerson Shigeru
Griesi-Oliveira, Karina
Musso, Camila Manso
Amaral, Murilo Sena
Silva, Lucas Ferreira da
Astray, Renato Mancini
Suarez-Patino, Sandra Fernanda
Ventini, Daniella Cristina
Silva, Sergio Gomes da
Yamamoto, Guilherme Lopes
Ezquina, Suzana
Naslavsky, Michel Satya
Telles-Silva, Kayque Alves
Weinmann, Karina
van der Linden, Vanessa
van der Linden, Helio
Oliveira, Joao Ricardo Mendes de
Arrais, Nivia Maria Rodrigues
Melo, Adriana
Figueiredo, Thalita
Santos, Silvana
Meira, Joanna Goes Castro
Passos, Saulo Duarte
Almeida, Roque Pacheco de
Bispo, Ana Jovina Barreto
Cavalheiro, Esper Abrão [UNIFESP]
Kalil, Jorge
Cunha-Neto, Edecio
Nakaya, Helder
Andreata-Santos, Robert
Ferreira, Luis Carlos de Souza
Verjovski-Almeida, Sergio
Ho, Paulo Lee
Passos-Bueno, Maria Rita
Zatz, Mayana
description Congenital Zika syndrome (CZS) causes early brain development impairment by affecting neural progenitor cells (NPCs). Here, we analyze NPCs from three pairs of dizygotic twins discordant for CZS. We compare by RNA-Seq the NPCs derived from CZS-affected and CZS unaffected twins. Prior to Zika virus (ZIKV) infection the NPCs from CZS babies show a significantly different gene expression signature of mTOR and Wnt pathway regulators, key to a neurodevelopmental program. Following ZIKV in vitro infection, cells from affected individuals have significantly higher ZIKV replication and reduced cell growth. Whole-exome analysis in 18 affected CZS babies as compared to 5 unaffected twins and 609 controls excludes a monogenic model to explain resistance or increased susceptibility to CZS development. Overall, our results indicate that CZS is not a stochastic event and depends on NPC intrinsic susceptibility, possibly related to oligogenic and/or epigenetic mechanisms.
publishDate 2018
dc.date.none.fl_str_mv 2018
2020-07-20T16:31:15Z
2020-07-20T16:31:15Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/s41467-017-02790-9
Nature Communications. London, v. 9, 2018.
10.1038/s41467-017-02790-9
WOS000427248800003a.pdf
2041-1723
https://repositorio.unifesp.br/handle/11600/55823
WOS:000427248800003a
url http://dx.doi.org/10.1038/s41467-017-02790-9
https://repositorio.unifesp.br/handle/11600/55823
identifier_str_mv Nature Communications. London, v. 9, 2018.
10.1038/s41467-017-02790-9
WOS000427248800003a.pdf
2041-1723
WOS:000427248800003a
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nature Communications
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application/pdf
dc.coverage.none.fl_str_mv London
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
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institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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