Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies

Detalhes bibliográficos
Autor(a) principal: Straus, A. H.
Data de Publicação: 1996
Outros Autores: Freymuller, E., Travassos, Luiz Rodolpho [UNIFESP], Takahashi, H. K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1080/02681219680000301
http://repositorio.unifesp.br/handle/11600/44492
Resumo: Two monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface.
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spelling Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodiesglycoproteinimmunoelectron microscopymonoclonal antibodiesParacoccidioides brasiliensisTwo monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface.UNIV FED SAO PAULO,EPM,CTR ELECTRON MICROSCOPY,BR-04023900 SAO PAULO,SP,BRAZILUNIV FED SAO PAULO,EPM,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023900 SAO PAULO,SP,BRAZILUNIV FED SAO PAULO,EPM,CTR ELECTRON MICROSCOPY,BR-04023900 SAO PAULO,SP,BRAZILUNIV FED SAO PAULO,EPM,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023900 SAO PAULO,SP,BRAZILWeb of ScienceBlackwell Science LtdUniversidade Federal de São Paulo (UNIFESP)Straus, A. H.Freymuller, E.Travassos, Luiz Rodolpho [UNIFESP]Takahashi, H. K.2018-06-15T18:07:24Z2018-06-15T18:07:24Z1996-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion181-186http://dx.doi.org/10.1080/02681219680000301Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996.10.1080/026812196800003010268-1218http://repositorio.unifesp.br/handle/11600/44492WOS:A1996UN54900005engJournal Of Medical And Veterinary Mycologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T11:58:48Zoai:repositorio.unifesp.br/:11600/44492Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T11:58:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
title Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
spellingShingle Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
Straus, A. H.
glycoprotein
immunoelectron microscopy
monoclonal antibodies
Paracoccidioides brasiliensis
title_short Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
title_full Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
title_fullStr Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
title_full_unstemmed Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
title_sort Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
author Straus, A. H.
author_facet Straus, A. H.
Freymuller, E.
Travassos, Luiz Rodolpho [UNIFESP]
Takahashi, H. K.
author_role author
author2 Freymuller, E.
Travassos, Luiz Rodolpho [UNIFESP]
Takahashi, H. K.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Straus, A. H.
Freymuller, E.
Travassos, Luiz Rodolpho [UNIFESP]
Takahashi, H. K.
dc.subject.por.fl_str_mv glycoprotein
immunoelectron microscopy
monoclonal antibodies
Paracoccidioides brasiliensis
topic glycoprotein
immunoelectron microscopy
monoclonal antibodies
Paracoccidioides brasiliensis
description Two monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface.
publishDate 1996
dc.date.none.fl_str_mv 1996-06-01
2018-06-15T18:07:24Z
2018-06-15T18:07:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/02681219680000301
Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996.
10.1080/02681219680000301
0268-1218
http://repositorio.unifesp.br/handle/11600/44492
WOS:A1996UN54900005
url http://dx.doi.org/10.1080/02681219680000301
http://repositorio.unifesp.br/handle/11600/44492
identifier_str_mv Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996.
10.1080/02681219680000301
0268-1218
WOS:A1996UN54900005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Medical And Veterinary Mycology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 181-186
dc.publisher.none.fl_str_mv Blackwell Science Ltd
publisher.none.fl_str_mv Blackwell Science Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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