Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1080/02681219680000301 http://repositorio.unifesp.br/handle/11600/44492 |
Resumo: | Two monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface. |
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Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodiesglycoproteinimmunoelectron microscopymonoclonal antibodiesParacoccidioides brasiliensisTwo monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface.UNIV FED SAO PAULO,EPM,CTR ELECTRON MICROSCOPY,BR-04023900 SAO PAULO,SP,BRAZILUNIV FED SAO PAULO,EPM,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023900 SAO PAULO,SP,BRAZILUNIV FED SAO PAULO,EPM,CTR ELECTRON MICROSCOPY,BR-04023900 SAO PAULO,SP,BRAZILUNIV FED SAO PAULO,EPM,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023900 SAO PAULO,SP,BRAZILWeb of ScienceBlackwell Science LtdUniversidade Federal de São Paulo (UNIFESP)Straus, A. H.Freymuller, E.Travassos, Luiz Rodolpho [UNIFESP]Takahashi, H. K.2018-06-15T18:07:24Z2018-06-15T18:07:24Z1996-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion181-186http://dx.doi.org/10.1080/02681219680000301Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996.10.1080/026812196800003010268-1218http://repositorio.unifesp.br/handle/11600/44492WOS:A1996UN54900005engJournal Of Medical And Veterinary Mycologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T11:58:48Zoai:repositorio.unifesp.br/:11600/44492Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T11:58:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
title |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
spellingShingle |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies Straus, A. H. glycoprotein immunoelectron microscopy monoclonal antibodies Paracoccidioides brasiliensis |
title_short |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
title_full |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
title_fullStr |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
title_full_unstemmed |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
title_sort |
Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies |
author |
Straus, A. H. |
author_facet |
Straus, A. H. Freymuller, E. Travassos, Luiz Rodolpho [UNIFESP] Takahashi, H. K. |
author_role |
author |
author2 |
Freymuller, E. Travassos, Luiz Rodolpho [UNIFESP] Takahashi, H. K. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Straus, A. H. Freymuller, E. Travassos, Luiz Rodolpho [UNIFESP] Takahashi, H. K. |
dc.subject.por.fl_str_mv |
glycoprotein immunoelectron microscopy monoclonal antibodies Paracoccidioides brasiliensis |
topic |
glycoprotein immunoelectron microscopy monoclonal antibodies Paracoccidioides brasiliensis |
description |
Two monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-06-01 2018-06-15T18:07:24Z 2018-06-15T18:07:24Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/02681219680000301 Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996. 10.1080/02681219680000301 0268-1218 http://repositorio.unifesp.br/handle/11600/44492 WOS:A1996UN54900005 |
url |
http://dx.doi.org/10.1080/02681219680000301 http://repositorio.unifesp.br/handle/11600/44492 |
identifier_str_mv |
Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996. 10.1080/02681219680000301 0268-1218 WOS:A1996UN54900005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Medical And Veterinary Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
181-186 |
dc.publisher.none.fl_str_mv |
Blackwell Science Ltd |
publisher.none.fl_str_mv |
Blackwell Science Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268368375513088 |