Fungicidal activity of peptides encoded by immunoglobulin genes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-017-11396-6 https://repositorio.unifesp.br/handle/11600/57372 |
Resumo: | Evidence from previous works disclosed the antimicrobial, antiviral, anti-tumour and/or immunomodulatory activity exerted, through different mechanisms of action, by peptides expressed in the complementarity-determining regions or even in the constant region of antibodies, independently from their specificity and isotype. Presently, we report the selection, from available databases, of peptide sequences encoded by immunoglobulin genes for the evaluation of their potential biological activities. Synthetic peptides representing the translated products of J lambda and J heavy genes proved to act in vitro against pathogenic fungi, entering yeast cells and causing their death, and exerted a therapeutic effect in a Galleria mellonella model of infection by Candida albicans. No haemolytic, cytotoxic and genotoxic effects were observed on mammalian cells. These findings raise the hypothesis that antibodies could be the evolutionary result of the adaptive combination of gene products ancestrally devoted to innate antimicrobial immunity. |
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Fungicidal activity of peptides encoded by immunoglobulin genesEvidence from previous works disclosed the antimicrobial, antiviral, anti-tumour and/or immunomodulatory activity exerted, through different mechanisms of action, by peptides expressed in the complementarity-determining regions or even in the constant region of antibodies, independently from their specificity and isotype. Presently, we report the selection, from available databases, of peptide sequences encoded by immunoglobulin genes for the evaluation of their potential biological activities. Synthetic peptides representing the translated products of J lambda and J heavy genes proved to act in vitro against pathogenic fungi, entering yeast cells and causing their death, and exerted a therapeutic effect in a Galleria mellonella model of infection by Candida albicans. No haemolytic, cytotoxic and genotoxic effects were observed on mammalian cells. These findings raise the hypothesis that antibodies could be the evolutionary result of the adaptive combination of gene products ancestrally devoted to innate antimicrobial immunity.Univ Parma, Dept Med & Surg, Parma, ItalyUniv Parma, Dept Chem Life Sci & Environm Sustainabil, Parma, ItalyFed Univ Sao Paulo UNIFESP, Expt Oncol Unit, Sao Paulo, BrazilFed Univ Sao Paulo UNIFESP, Expt Oncol Unit, Sao Paulo, BrazilWeb of ScienceNature Publishing Group2020-08-04T13:40:12Z2020-08-04T13:40:12Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.1038/s41598-017-11396-6Scientific Reports. London, v. 7, p. -, 2017.10.1038/s41598-017-11396-6WOS000409562000043.pdf2045-2322https://repositorio.unifesp.br/handle/11600/57372WOS:000409562000043engScientific ReportsLondoninfo:eu-repo/semantics/openAccessPolonelli, LucianoCiociola, TeclaSperinde, MartinaGiovati, LauraD'Adda, TizianaGalati, SerenaTravassos, Luiz R. [UNIFESP]Magliani, WalterConti, Stefaniareponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T05:30:05Zoai:repositorio.unifesp.br/:11600/57372Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T05:30:05Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Fungicidal activity of peptides encoded by immunoglobulin genes |
title |
Fungicidal activity of peptides encoded by immunoglobulin genes |
spellingShingle |
Fungicidal activity of peptides encoded by immunoglobulin genes Polonelli, Luciano |
title_short |
Fungicidal activity of peptides encoded by immunoglobulin genes |
title_full |
Fungicidal activity of peptides encoded by immunoglobulin genes |
title_fullStr |
Fungicidal activity of peptides encoded by immunoglobulin genes |
title_full_unstemmed |
Fungicidal activity of peptides encoded by immunoglobulin genes |
title_sort |
Fungicidal activity of peptides encoded by immunoglobulin genes |
author |
Polonelli, Luciano |
author_facet |
Polonelli, Luciano Ciociola, Tecla Sperinde, Martina Giovati, Laura D'Adda, Tiziana Galati, Serena Travassos, Luiz R. [UNIFESP] Magliani, Walter Conti, Stefania |
author_role |
author |
author2 |
Ciociola, Tecla Sperinde, Martina Giovati, Laura D'Adda, Tiziana Galati, Serena Travassos, Luiz R. [UNIFESP] Magliani, Walter Conti, Stefania |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Polonelli, Luciano Ciociola, Tecla Sperinde, Martina Giovati, Laura D'Adda, Tiziana Galati, Serena Travassos, Luiz R. [UNIFESP] Magliani, Walter Conti, Stefania |
description |
Evidence from previous works disclosed the antimicrobial, antiviral, anti-tumour and/or immunomodulatory activity exerted, through different mechanisms of action, by peptides expressed in the complementarity-determining regions or even in the constant region of antibodies, independently from their specificity and isotype. Presently, we report the selection, from available databases, of peptide sequences encoded by immunoglobulin genes for the evaluation of their potential biological activities. Synthetic peptides representing the translated products of J lambda and J heavy genes proved to act in vitro against pathogenic fungi, entering yeast cells and causing their death, and exerted a therapeutic effect in a Galleria mellonella model of infection by Candida albicans. No haemolytic, cytotoxic and genotoxic effects were observed on mammalian cells. These findings raise the hypothesis that antibodies could be the evolutionary result of the adaptive combination of gene products ancestrally devoted to innate antimicrobial immunity. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2020-08-04T13:40:12Z 2020-08-04T13:40:12Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-017-11396-6 Scientific Reports. London, v. 7, p. -, 2017. 10.1038/s41598-017-11396-6 WOS000409562000043.pdf 2045-2322 https://repositorio.unifesp.br/handle/11600/57372 WOS:000409562000043 |
url |
http://dx.doi.org/10.1038/s41598-017-11396-6 https://repositorio.unifesp.br/handle/11600/57372 |
identifier_str_mv |
Scientific Reports. London, v. 7, p. -, 2017. 10.1038/s41598-017-11396-6 WOS000409562000043.pdf 2045-2322 WOS:000409562000043 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.coverage.none.fl_str_mv |
London |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268460505497600 |