Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35669 http://dx.doi.org/10.1111/j.1751-1097.2012.01214.x |
Resumo: | It remains unknown if the oxidative stress can be regulated by low-level laser therapy (LLLT) in lung inflammation induced by intestinal reperfusion (i-I/R). A study was developed in which rats were irradiated (660 nm, 30 mW, 5.4 J) on the skin over the bronchus and euthanized 2 h after the initial of intestinal reperfusion. Lung edema and bronchoalveolar lavage fluid neutrophils were measured by the Evans blue extravasation and myeloperoxidase (MPO) activity respectively. Lung histology was used for analyzing the injury score. Reactive oxygen species (ROS) was measured by fluorescence. Both expression intercellular adhesion molecule 1 (ICAM-1) and peroxisome proliferator-activated receptor-y (PPARy) were measured by RT-PCR. the lung immunohistochemical localization of ICAM-1 was visualized as a brown stain. Both lung HSP70 and glutathione protein were evaluated by ELISA. LLLT reduced neatly the edema, neutrophils influx, MPO activity and ICAM-1 mRNA expression. LLLT also reduced the ROS formation and oppositely increased GSH concentration in lung from i-I/R groups. Both HSP70 and PPARy expression also were elevated after laser irradiation. Results indicate that laser effect in attenuating the acute lung inflammation is driven to restore the balance between the pro- and antioxidants mediators rising of PPARy expression and consequently the HSP70 production. |
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Lima, Flavia Mafra deAlbertini, RegianeDantas, YvanaMaia-Filho, Antonio LuisSantana, Cristiano de LouraCastro-Faria-Neto, Hugo CaireFranca, CristianeVillaverde, Antonio BalbinAimbire, Flavio [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Nove Julho UNINOVEIntes Care Unity Hosp & MaternFac Integral Diferencial FACIDFiocruz MSUNICASTELO2016-01-24T14:30:51Z2016-01-24T14:30:51Z2013-01-01Photochemistry and Photobiology. Hoboken: Wiley-Blackwell, v. 89, n. 1, p. 179-188, 2013.0031-8655http://repositorio.unifesp.br/handle/11600/35669http://dx.doi.org/10.1111/j.1751-1097.2012.01214.x10.1111/j.1751-1097.2012.01214.xWOS:000312990200023It remains unknown if the oxidative stress can be regulated by low-level laser therapy (LLLT) in lung inflammation induced by intestinal reperfusion (i-I/R). A study was developed in which rats were irradiated (660 nm, 30 mW, 5.4 J) on the skin over the bronchus and euthanized 2 h after the initial of intestinal reperfusion. Lung edema and bronchoalveolar lavage fluid neutrophils were measured by the Evans blue extravasation and myeloperoxidase (MPO) activity respectively. Lung histology was used for analyzing the injury score. Reactive oxygen species (ROS) was measured by fluorescence. Both expression intercellular adhesion molecule 1 (ICAM-1) and peroxisome proliferator-activated receptor-y (PPARy) were measured by RT-PCR. the lung immunohistochemical localization of ICAM-1 was visualized as a brown stain. Both lung HSP70 and glutathione protein were evaluated by ELISA. LLLT reduced neatly the edema, neutrophils influx, MPO activity and ICAM-1 mRNA expression. LLLT also reduced the ROS formation and oppositely increased GSH concentration in lung from i-I/R groups. Both HSP70 and PPARy expression also were elevated after laser irradiation. Results indicate that laser effect in attenuating the acute lung inflammation is driven to restore the balance between the pro- and antioxidants mediators rising of PPARy expression and consequently the HSP70 production.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fed Univ São Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP, BrazilUniv Nove Julho UNINOVE, Dept Rehabil Sci, São Paulo, BrazilIntes Care Unity Hosp & Matern, Teresina, PI, BrazilFac Integral Diferencial FACID, Teresina, PI, BrazilFiocruz MS, Lab Immunopharmacol, IOC, BR-21045900 Rio de Janeiro, RJ, BrazilUniv Nove Julho UNINOVE, Res Grp Biophoton, São Paulo, BrazilUNICASTELO, Inst Biomed Engn, Sao Jose Dos Campos, SP, BrazilFed Univ São Paulo UNIFESP, Dept Sci & Technol, Sao Jose Dos Campos, SP, BrazilFAPESP: 2008/08838-5Web of Science179-188engWiley-BlackwellPhotochemistry and Photobiologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessLow-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/356692021-09-29 09:31:00.907metadata only accessoai:repositorio.unifesp.br:11600/35669Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-29T12:31Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
title |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
spellingShingle |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion Lima, Flavia Mafra de |
title_short |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
title_full |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
title_fullStr |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
title_full_unstemmed |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
title_sort |
Low-Level Laser Therapy Restores the Oxidative Stress Balance in Acute Lung Injury Induced by Gut Ischemia and Reperfusion |
author |
Lima, Flavia Mafra de |
author_facet |
Lima, Flavia Mafra de Albertini, Regiane Dantas, Yvana Maia-Filho, Antonio Luis Santana, Cristiano de Loura Castro-Faria-Neto, Hugo Caire Franca, Cristiane Villaverde, Antonio Balbin Aimbire, Flavio [UNIFESP] |
author_role |
author |
author2 |
Albertini, Regiane Dantas, Yvana Maia-Filho, Antonio Luis Santana, Cristiano de Loura Castro-Faria-Neto, Hugo Caire Franca, Cristiane Villaverde, Antonio Balbin Aimbire, Flavio [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Nove Julho UNINOVE Intes Care Unity Hosp & Matern Fac Integral Diferencial FACID Fiocruz MS UNICASTELO |
dc.contributor.author.fl_str_mv |
Lima, Flavia Mafra de Albertini, Regiane Dantas, Yvana Maia-Filho, Antonio Luis Santana, Cristiano de Loura Castro-Faria-Neto, Hugo Caire Franca, Cristiane Villaverde, Antonio Balbin Aimbire, Flavio [UNIFESP] |
description |
It remains unknown if the oxidative stress can be regulated by low-level laser therapy (LLLT) in lung inflammation induced by intestinal reperfusion (i-I/R). A study was developed in which rats were irradiated (660 nm, 30 mW, 5.4 J) on the skin over the bronchus and euthanized 2 h after the initial of intestinal reperfusion. Lung edema and bronchoalveolar lavage fluid neutrophils were measured by the Evans blue extravasation and myeloperoxidase (MPO) activity respectively. Lung histology was used for analyzing the injury score. Reactive oxygen species (ROS) was measured by fluorescence. Both expression intercellular adhesion molecule 1 (ICAM-1) and peroxisome proliferator-activated receptor-y (PPARy) were measured by RT-PCR. the lung immunohistochemical localization of ICAM-1 was visualized as a brown stain. Both lung HSP70 and glutathione protein were evaluated by ELISA. LLLT reduced neatly the edema, neutrophils influx, MPO activity and ICAM-1 mRNA expression. LLLT also reduced the ROS formation and oppositely increased GSH concentration in lung from i-I/R groups. Both HSP70 and PPARy expression also were elevated after laser irradiation. Results indicate that laser effect in attenuating the acute lung inflammation is driven to restore the balance between the pro- and antioxidants mediators rising of PPARy expression and consequently the HSP70 production. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:30:51Z |
dc.date.available.fl_str_mv |
2016-01-24T14:30:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Photochemistry and Photobiology. Hoboken: Wiley-Blackwell, v. 89, n. 1, p. 179-188, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/35669 http://dx.doi.org/10.1111/j.1751-1097.2012.01214.x |
dc.identifier.issn.none.fl_str_mv |
0031-8655 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1751-1097.2012.01214.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000312990200023 |
identifier_str_mv |
Photochemistry and Photobiology. Hoboken: Wiley-Blackwell, v. 89, n. 1, p. 179-188, 2013. 0031-8655 10.1111/j.1751-1097.2012.01214.x WOS:000312990200023 |
url |
http://repositorio.unifesp.br/handle/11600/35669 http://dx.doi.org/10.1111/j.1751-1097.2012.01214.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Photochemistry and Photobiology |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
179-188 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1802764133447237632 |