Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

Detalhes bibliográficos
Autor(a) principal: Endo, S.
Data de Publicação: 2004
Outros Autores: Komori, T., Ricci, Giannina [UNIFESP], Sano, A., Yokoyama, K., Ohori, A., Kamei, K., Franco, Marcello Fabiano de [UNIFESP], Miyaji, M., Nishimura, K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/27715
http://dx.doi.org/10.1016/j.femsle.2004.03.015
Resumo: Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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spelling Endo, S.Komori, T.Ricci, Giannina [UNIFESP]Sano, A.Yokoyama, K.Ohori, A.Kamei, K.Franco, Marcello Fabiano de [UNIFESP]Miyaji, M.Nishimura, K.Chiba UnivFujisawa Pharmaceut Co LtdUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:37:07Z2016-01-24T12:37:07Z2004-05-01Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.0378-1097http://repositorio.unifesp.br/handle/11600/27715http://dx.doi.org/10.1016/j.femsle.2004.03.01510.1016/j.femsle.2004.03.015WOS:000221218300013Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, JapanFujisawa Pharmaceut Co Ltd, Analyt Res Labs, Osaka, JapanUniversidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, BrazilUniversidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, BrazilWeb of Science93-97engElsevier B.V.Fems Microbiology Lettershttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessgp43 geneParacoccidioides brasiliensisparacoccidioidomycosisDetection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) methodinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/277152022-07-08 10:45:08.572metadata only accessoai:repositorio.unifesp.br:11600/27715Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-08T13:45:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
title Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
spellingShingle Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
Endo, S.
gp43 gene
Paracoccidioides brasiliensis
paracoccidioidomycosis
title_short Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
title_full Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
title_fullStr Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
title_full_unstemmed Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
title_sort Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
author Endo, S.
author_facet Endo, S.
Komori, T.
Ricci, Giannina [UNIFESP]
Sano, A.
Yokoyama, K.
Ohori, A.
Kamei, K.
Franco, Marcello Fabiano de [UNIFESP]
Miyaji, M.
Nishimura, K.
author_role author
author2 Komori, T.
Ricci, Giannina [UNIFESP]
Sano, A.
Yokoyama, K.
Ohori, A.
Kamei, K.
Franco, Marcello Fabiano de [UNIFESP]
Miyaji, M.
Nishimura, K.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Chiba Univ
Fujisawa Pharmaceut Co Ltd
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Endo, S.
Komori, T.
Ricci, Giannina [UNIFESP]
Sano, A.
Yokoyama, K.
Ohori, A.
Kamei, K.
Franco, Marcello Fabiano de [UNIFESP]
Miyaji, M.
Nishimura, K.
dc.subject.eng.fl_str_mv gp43 gene
Paracoccidioides brasiliensis
paracoccidioidomycosis
topic gp43 gene
Paracoccidioides brasiliensis
paracoccidioidomycosis
description Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
publishDate 2004
dc.date.issued.fl_str_mv 2004-05-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:37:07Z
dc.date.available.fl_str_mv 2016-01-24T12:37:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/27715
http://dx.doi.org/10.1016/j.femsle.2004.03.015
dc.identifier.issn.none.fl_str_mv 0378-1097
dc.identifier.doi.none.fl_str_mv 10.1016/j.femsle.2004.03.015
dc.identifier.wos.none.fl_str_mv WOS:000221218300013
identifier_str_mv Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.
0378-1097
10.1016/j.femsle.2004.03.015
WOS:000221218300013
url http://repositorio.unifesp.br/handle/11600/27715
http://dx.doi.org/10.1016/j.femsle.2004.03.015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Fems Microbiology Letters
dc.rights.driver.fl_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 93-97
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1802764150251716608

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