Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/27715 http://dx.doi.org/10.1016/j.femsle.2004.03.015 |
Resumo: | Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
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Endo, S.Komori, T.Ricci, Giannina [UNIFESP]Sano, A.Yokoyama, K.Ohori, A.Kamei, K.Franco, Marcello Fabiano de [UNIFESP]Miyaji, M.Nishimura, K.Chiba UnivFujisawa Pharmaceut Co LtdUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:37:07Z2016-01-24T12:37:07Z2004-05-01Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.0378-1097http://repositorio.unifesp.br/handle/11600/27715http://dx.doi.org/10.1016/j.femsle.2004.03.01510.1016/j.femsle.2004.03.015WOS:000221218300013Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, JapanFujisawa Pharmaceut Co Ltd, Analyt Res Labs, Osaka, JapanUniversidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, BrazilUniversidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, BrazilWeb of Science93-97engElsevier B.V.Fems Microbiology Lettershttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessgp43 geneParacoccidioides brasiliensisparacoccidioidomycosisDetection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) methodinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/277152022-07-08 10:45:08.572metadata only accessoai:repositorio.unifesp.br:11600/27715Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-08T13:45:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
title |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
spellingShingle |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method Endo, S. gp43 gene Paracoccidioides brasiliensis paracoccidioidomycosis |
title_short |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
title_full |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
title_fullStr |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
title_full_unstemmed |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
title_sort |
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method |
author |
Endo, S. |
author_facet |
Endo, S. Komori, T. Ricci, Giannina [UNIFESP] Sano, A. Yokoyama, K. Ohori, A. Kamei, K. Franco, Marcello Fabiano de [UNIFESP] Miyaji, M. Nishimura, K. |
author_role |
author |
author2 |
Komori, T. Ricci, Giannina [UNIFESP] Sano, A. Yokoyama, K. Ohori, A. Kamei, K. Franco, Marcello Fabiano de [UNIFESP] Miyaji, M. Nishimura, K. |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Chiba Univ Fujisawa Pharmaceut Co Ltd Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Endo, S. Komori, T. Ricci, Giannina [UNIFESP] Sano, A. Yokoyama, K. Ohori, A. Kamei, K. Franco, Marcello Fabiano de [UNIFESP] Miyaji, M. Nishimura, K. |
dc.subject.eng.fl_str_mv |
gp43 gene Paracoccidioides brasiliensis paracoccidioidomycosis |
topic |
gp43 gene Paracoccidioides brasiliensis paracoccidioidomycosis |
description |
Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-05-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:37:07Z |
dc.date.available.fl_str_mv |
2016-01-24T12:37:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/27715 http://dx.doi.org/10.1016/j.femsle.2004.03.015 |
dc.identifier.issn.none.fl_str_mv |
0378-1097 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.femsle.2004.03.015 |
dc.identifier.wos.none.fl_str_mv |
WOS:000221218300013 |
identifier_str_mv |
Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004. 0378-1097 10.1016/j.femsle.2004.03.015 WOS:000221218300013 |
url |
http://repositorio.unifesp.br/handle/11600/27715 http://dx.doi.org/10.1016/j.femsle.2004.03.015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Fems Microbiology Letters |
dc.rights.driver.fl_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
93-97 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1802764150251716608 |