Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats

Detalhes bibliográficos
Autor(a) principal: Kracochansky, Mirian [UNIFESP]
Data de Publicação: 2012
Outros Autores: Reis, Leonardo Oliveira, Lorenzetti, Fábio [UNIFESP], Ortiz, Valdemar [UNIFESP], Dambros, Miriam [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1677-55382012000200017
http://repositorio.unifesp.br/handle/11600/7056
Resumo: OBJECTIVE: To analyze the impact of low levels of testosterone induced by orchiectomy and the effect of alpha-tocopherol supplementation on oxidative stress in the urethral sphincter. MATERIALS AND METHODS: Forty male Wistar rats weighing 250-300g were divided into four groups with 10 each: Sham group; Orchiectomy group: bilateral orchiectomy; Orchiectomy-pre-Tocopherol group: bilateral orchiectomy preceded by alpha-tocopherol supplementation for four weeks; Orchiectomy-full-Tocopherol group: bilateral orchiectomy with alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the protocol end, animals were euthanized and had the sphincter analyzed stereologically focusing on collagen and muscle fibers percentage. Oxidative stress levels were determined using 8-epi-PGF2. RESULTS: The 8-epi-PGF2 levels were statistically higher (p < 0.0003) in the Orchiectomy group compared to others groups while Sham and Orchiectomy-full-Tocopherol groups presented statistically similar values (p = 0.52). Collagen volumetric densities were significantly lower in Sham and Orchiectomy-full-Tocopherol groups (p < 0.022). Sham group presented statistically greater muscle fiber percent. CONCLUSION: Castration caused oxidative stress in the urethral sphincter complex, with increased collagen deposition. Alpha-tocopherol had a protective effect and its supplementation for twelve weeks provided the greatest protection.
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spelling Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of ratsapoptosisalpha-tocopheroloxidative stressantioxidantscastrationOBJECTIVE: To analyze the impact of low levels of testosterone induced by orchiectomy and the effect of alpha-tocopherol supplementation on oxidative stress in the urethral sphincter. MATERIALS AND METHODS: Forty male Wistar rats weighing 250-300g were divided into four groups with 10 each: Sham group; Orchiectomy group: bilateral orchiectomy; Orchiectomy-pre-Tocopherol group: bilateral orchiectomy preceded by alpha-tocopherol supplementation for four weeks; Orchiectomy-full-Tocopherol group: bilateral orchiectomy with alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the protocol end, animals were euthanized and had the sphincter analyzed stereologically focusing on collagen and muscle fibers percentage. Oxidative stress levels were determined using 8-epi-PGF2. RESULTS: The 8-epi-PGF2 levels were statistically higher (p < 0.0003) in the Orchiectomy group compared to others groups while Sham and Orchiectomy-full-Tocopherol groups presented statistically similar values (p = 0.52). Collagen volumetric densities were significantly lower in Sham and Orchiectomy-full-Tocopherol groups (p < 0.022). Sham group presented statistically greater muscle fiber percent. CONCLUSION: Castration caused oxidative stress in the urethral sphincter complex, with increased collagen deposition. Alpha-tocopherol had a protective effect and its supplementation for twelve weeks provided the greatest protection.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Division of Geriatric UrologyUniversity of Campinas School of Medical Sciences Division of UrologyUNIFESP, EPM, Division of Geriatric UrologySciELOSociedade Brasileira de UrologiaUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Kracochansky, Mirian [UNIFESP]Reis, Leonardo OliveiraLorenzetti, Fábio [UNIFESP]Ortiz, Valdemar [UNIFESP]Dambros, Miriam [UNIFESP]2015-06-14T13:43:42Z2015-06-14T13:43:42Z2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion277-283application/pdfhttp://dx.doi.org/10.1590/S1677-55382012000200017International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 2, p. 277-283, 2012.10.1590/S1677-55382012000200017S1677-55382012000200017.pdf1677-5538S1677-55382012000200017http://repositorio.unifesp.br/handle/11600/7056WOS:000306550800033engInternational braz j urolinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T04:30:06Zoai:repositorio.unifesp.br/:11600/7056Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T04:30:06Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
title Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
spellingShingle Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
Kracochansky, Mirian [UNIFESP]
apoptosis
alpha-tocopherol
oxidative stress
antioxidants
castration
title_short Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
title_full Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
title_fullStr Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
title_full_unstemmed Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
title_sort Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
author Kracochansky, Mirian [UNIFESP]
author_facet Kracochansky, Mirian [UNIFESP]
Reis, Leonardo Oliveira
Lorenzetti, Fábio [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Dambros, Miriam [UNIFESP]
author_role author
author2 Reis, Leonardo Oliveira
Lorenzetti, Fábio [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Dambros, Miriam [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Kracochansky, Mirian [UNIFESP]
Reis, Leonardo Oliveira
Lorenzetti, Fábio [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Dambros, Miriam [UNIFESP]
dc.subject.por.fl_str_mv apoptosis
alpha-tocopherol
oxidative stress
antioxidants
castration
topic apoptosis
alpha-tocopherol
oxidative stress
antioxidants
castration
description OBJECTIVE: To analyze the impact of low levels of testosterone induced by orchiectomy and the effect of alpha-tocopherol supplementation on oxidative stress in the urethral sphincter. MATERIALS AND METHODS: Forty male Wistar rats weighing 250-300g were divided into four groups with 10 each: Sham group; Orchiectomy group: bilateral orchiectomy; Orchiectomy-pre-Tocopherol group: bilateral orchiectomy preceded by alpha-tocopherol supplementation for four weeks; Orchiectomy-full-Tocopherol group: bilateral orchiectomy with alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the protocol end, animals were euthanized and had the sphincter analyzed stereologically focusing on collagen and muscle fibers percentage. Oxidative stress levels were determined using 8-epi-PGF2. RESULTS: The 8-epi-PGF2 levels were statistically higher (p < 0.0003) in the Orchiectomy group compared to others groups while Sham and Orchiectomy-full-Tocopherol groups presented statistically similar values (p = 0.52). Collagen volumetric densities were significantly lower in Sham and Orchiectomy-full-Tocopherol groups (p < 0.022). Sham group presented statistically greater muscle fiber percent. CONCLUSION: Castration caused oxidative stress in the urethral sphincter complex, with increased collagen deposition. Alpha-tocopherol had a protective effect and its supplementation for twelve weeks provided the greatest protection.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
2015-06-14T13:43:42Z
2015-06-14T13:43:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1677-55382012000200017
International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 2, p. 277-283, 2012.
10.1590/S1677-55382012000200017
S1677-55382012000200017.pdf
1677-5538
S1677-55382012000200017
http://repositorio.unifesp.br/handle/11600/7056
WOS:000306550800033
url http://dx.doi.org/10.1590/S1677-55382012000200017
http://repositorio.unifesp.br/handle/11600/7056
identifier_str_mv International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 2, p. 277-283, 2012.
10.1590/S1677-55382012000200017
S1677-55382012000200017.pdf
1677-5538
S1677-55382012000200017
WOS:000306550800033
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International braz j urol
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 277-283
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Urologia
publisher.none.fl_str_mv Sociedade Brasileira de Urologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268314564689920

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