Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats

Detalhes bibliográficos
Autor(a) principal: Fartes, Guilherme [UNIFESP]
Data de Publicação: 2012
Outros Autores: Lorenzetti, Fábio [UNIFESP], Salvador, Larissa Beloti [UNIFESP], Ortiz, Valdemar [UNIFESP], Dambros, Miriam [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1677-55382012000300016
http://repositorio.unifesp.br/handle/11600/7128
Resumo: OBJECTIVE: The purpose of this study was to compare the effects of castration on cell death rate of the adult rat prostates and to evaluate the benefic action of alpha tocopherol supplementation to avoid apoptosis post-orchiectomy. MATERIAL AND METHODS: Thirty male Wistar rats weighing 250-300g were divided into three groups: group I - they were subjected to bilateral orchiectomy and sacrificed eight weeks after the procedure; group II - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure; and group III - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the end of the experiment, the prostatectomy was performed in all rats. The presence of oxidative stress was determined by assaying the blood level of 8-isoprostane and the occurrence of apoptosis was evaluated by identification of active caspase-3 through immunohistochemical analysis. RESULTS: The statistic analysis of active caspase-3 showed that in the long-term castrated group the detection was higher than in groups were the alpha-tocopherol was supplemented (p=0.007). Analysis of 8-isoprostane levels showed higher concentrations of reactive oxygen species in group I compared to other groups (p<0.05). Groups II and III presented active caspase-3 lower than in group I (p<0.05). CONCLUSION: Our exploratory analyses demonstrate a method to study the aging process and its influence on oxidative stress of prostatic tissue and cells death rate. Based on our results we can suggest that alpha tocopherol supplementation can decrease the apoptotic process as well as the oxidative stress levels induced by androgen deprivation of the prostate gland.
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spelling Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated ratsProstateTestosteroneOxidative Stressalpha-TocopherolApoptosisOBJECTIVE: The purpose of this study was to compare the effects of castration on cell death rate of the adult rat prostates and to evaluate the benefic action of alpha tocopherol supplementation to avoid apoptosis post-orchiectomy. MATERIAL AND METHODS: Thirty male Wistar rats weighing 250-300g were divided into three groups: group I - they were subjected to bilateral orchiectomy and sacrificed eight weeks after the procedure; group II - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure; and group III - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the end of the experiment, the prostatectomy was performed in all rats. The presence of oxidative stress was determined by assaying the blood level of 8-isoprostane and the occurrence of apoptosis was evaluated by identification of active caspase-3 through immunohistochemical analysis. RESULTS: The statistic analysis of active caspase-3 showed that in the long-term castrated group the detection was higher than in groups were the alpha-tocopherol was supplemented (p=0.007). Analysis of 8-isoprostane levels showed higher concentrations of reactive oxygen species in group I compared to other groups (p<0.05). Groups II and III presented active caspase-3 lower than in group I (p<0.05). CONCLUSION: Our exploratory analyses demonstrate a method to study the aging process and its influence on oxidative stress of prostatic tissue and cells death rate. Based on our results we can suggest that alpha tocopherol supplementation can decrease the apoptotic process as well as the oxidative stress levels induced by androgen deprivation of the prostate gland.Federal University of São PauloUNIFESP, EPM, São PauloSciELOSociedade Brasileira de UrologiaUniversidade Federal de São Paulo (UNIFESP)Fartes, Guilherme [UNIFESP]Lorenzetti, Fábio [UNIFESP]Salvador, Larissa Beloti [UNIFESP]Ortiz, Valdemar [UNIFESP]Dambros, Miriam [UNIFESP]2015-06-14T13:44:44Z2015-06-14T13:44:44Z2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion419-425application/pdfhttp://dx.doi.org/10.1590/S1677-55382012000300016International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 3, p. 419-425, 2012.10.1590/S1677-55382012000300016S1677-55382012000300016.pdf1677-5538S1677-55382012000300016http://repositorio.unifesp.br/handle/11600/7128WOS:000314415400027engInternational braz j urolinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T04:59:42Zoai:repositorio.unifesp.br/:11600/7128Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T04:59:42Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
title Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
spellingShingle Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
Fartes, Guilherme [UNIFESP]
Prostate
Testosterone
Oxidative Stress
alpha-Tocopherol
Apoptosis
title_short Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
title_full Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
title_fullStr Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
title_full_unstemmed Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
title_sort Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats
author Fartes, Guilherme [UNIFESP]
author_facet Fartes, Guilherme [UNIFESP]
Lorenzetti, Fábio [UNIFESP]
Salvador, Larissa Beloti [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Dambros, Miriam [UNIFESP]
author_role author
author2 Lorenzetti, Fábio [UNIFESP]
Salvador, Larissa Beloti [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Dambros, Miriam [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Fartes, Guilherme [UNIFESP]
Lorenzetti, Fábio [UNIFESP]
Salvador, Larissa Beloti [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Dambros, Miriam [UNIFESP]
dc.subject.por.fl_str_mv Prostate
Testosterone
Oxidative Stress
alpha-Tocopherol
Apoptosis
topic Prostate
Testosterone
Oxidative Stress
alpha-Tocopherol
Apoptosis
description OBJECTIVE: The purpose of this study was to compare the effects of castration on cell death rate of the adult rat prostates and to evaluate the benefic action of alpha tocopherol supplementation to avoid apoptosis post-orchiectomy. MATERIAL AND METHODS: Thirty male Wistar rats weighing 250-300g were divided into three groups: group I - they were subjected to bilateral orchiectomy and sacrificed eight weeks after the procedure; group II - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure; and group III - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the end of the experiment, the prostatectomy was performed in all rats. The presence of oxidative stress was determined by assaying the blood level of 8-isoprostane and the occurrence of apoptosis was evaluated by identification of active caspase-3 through immunohistochemical analysis. RESULTS: The statistic analysis of active caspase-3 showed that in the long-term castrated group the detection was higher than in groups were the alpha-tocopherol was supplemented (p=0.007). Analysis of 8-isoprostane levels showed higher concentrations of reactive oxygen species in group I compared to other groups (p<0.05). Groups II and III presented active caspase-3 lower than in group I (p<0.05). CONCLUSION: Our exploratory analyses demonstrate a method to study the aging process and its influence on oxidative stress of prostatic tissue and cells death rate. Based on our results we can suggest that alpha tocopherol supplementation can decrease the apoptotic process as well as the oxidative stress levels induced by androgen deprivation of the prostate gland.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
2015-06-14T13:44:44Z
2015-06-14T13:44:44Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1677-55382012000300016
International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 3, p. 419-425, 2012.
10.1590/S1677-55382012000300016
S1677-55382012000300016.pdf
1677-5538
S1677-55382012000300016
http://repositorio.unifesp.br/handle/11600/7128
WOS:000314415400027
url http://dx.doi.org/10.1590/S1677-55382012000300016
http://repositorio.unifesp.br/handle/11600/7128
identifier_str_mv International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 3, p. 419-425, 2012.
10.1590/S1677-55382012000300016
S1677-55382012000300016.pdf
1677-5538
S1677-55382012000300016
WOS:000314415400027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International braz j urol
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 419-425
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Urologia
publisher.none.fl_str_mv Sociedade Brasileira de Urologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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