Candida dubliniensis identification in Brazilian yeast stock collection
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762003000400020 http://repositorio.unifesp.br/handle/11600/1745 |
Resumo: | We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture. |
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Candida dubliniensis identification in Brazilian yeast stock collectionCandida dubliniensisoral candidiasisCandida spp.AidsWe investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture.Universidade Federal de São Paulo (UNIFESP) Laboratório Especial de MicologiaUniversidade Federal do Rio Grande do Norte Departamento de Microbiologia e ParasitologiaUNIFESP, Laboratório Especial de MicologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e ParasitologiaMariano, Priscilla de Laet Sant'ana [UNIFESP]Milan, Eveline Pípolo [UNIFESP]Matta, Daniel Archimedes da [UNIFESP]Colombo, Arnaldo Lopes [UNIFESP]2015-06-14T13:30:02Z2015-06-14T13:30:02Z2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion533-538application/pdfhttp://dx.doi.org/10.1590/S0074-02762003000400020Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003.10.1590/S0074-02762003000400020S0074-02762003000400020.pdf0074-0276S0074-02762003000400020http://repositorio.unifesp.br/handle/11600/1745WOS:000183993100020engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T03:05:48Zoai:repositorio.unifesp.br/:11600/1745Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T03:05:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Candida dubliniensis identification in Brazilian yeast stock collection |
title |
Candida dubliniensis identification in Brazilian yeast stock collection |
spellingShingle |
Candida dubliniensis identification in Brazilian yeast stock collection Mariano, Priscilla de Laet Sant'ana [UNIFESP] Candida dubliniensis oral candidiasis Candida spp. Aids |
title_short |
Candida dubliniensis identification in Brazilian yeast stock collection |
title_full |
Candida dubliniensis identification in Brazilian yeast stock collection |
title_fullStr |
Candida dubliniensis identification in Brazilian yeast stock collection |
title_full_unstemmed |
Candida dubliniensis identification in Brazilian yeast stock collection |
title_sort |
Candida dubliniensis identification in Brazilian yeast stock collection |
author |
Mariano, Priscilla de Laet Sant'ana [UNIFESP] |
author_facet |
Mariano, Priscilla de Laet Sant'ana [UNIFESP] Milan, Eveline Pípolo [UNIFESP] Matta, Daniel Archimedes da [UNIFESP] Colombo, Arnaldo Lopes [UNIFESP] |
author_role |
author |
author2 |
Milan, Eveline Pípolo [UNIFESP] Matta, Daniel Archimedes da [UNIFESP] Colombo, Arnaldo Lopes [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e Parasitologia |
dc.contributor.author.fl_str_mv |
Mariano, Priscilla de Laet Sant'ana [UNIFESP] Milan, Eveline Pípolo [UNIFESP] Matta, Daniel Archimedes da [UNIFESP] Colombo, Arnaldo Lopes [UNIFESP] |
dc.subject.por.fl_str_mv |
Candida dubliniensis oral candidiasis Candida spp. Aids |
topic |
Candida dubliniensis oral candidiasis Candida spp. Aids |
description |
We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-06-01 2015-06-14T13:30:02Z 2015-06-14T13:30:02Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762003000400020 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003. 10.1590/S0074-02762003000400020 S0074-02762003000400020.pdf 0074-0276 S0074-02762003000400020 http://repositorio.unifesp.br/handle/11600/1745 WOS:000183993100020 |
url |
http://dx.doi.org/10.1590/S0074-02762003000400020 http://repositorio.unifesp.br/handle/11600/1745 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003. 10.1590/S0074-02762003000400020 S0074-02762003000400020.pdf 0074-0276 S0074-02762003000400020 WOS:000183993100020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
533-538 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268423832600576 |