Candida dubliniensis identification in Brazilian yeast stock collection

Detalhes bibliográficos
Autor(a) principal: Mariano, Priscilla de Laet Sant'ana [UNIFESP]
Data de Publicação: 2003
Outros Autores: Milan, Eveline Pípolo [UNIFESP], Matta, Daniel Archimedes da [UNIFESP], Colombo, Arnaldo Lopes [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762003000400020
http://repositorio.unifesp.br/handle/11600/1745
Resumo: We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture.
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spelling Candida dubliniensis identification in Brazilian yeast stock collectionCandida dubliniensisoral candidiasisCandida spp.AidsWe investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture.Universidade Federal de São Paulo (UNIFESP) Laboratório Especial de MicologiaUniversidade Federal do Rio Grande do Norte Departamento de Microbiologia e ParasitologiaUNIFESP, Laboratório Especial de MicologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e ParasitologiaMariano, Priscilla de Laet Sant'ana [UNIFESP]Milan, Eveline Pípolo [UNIFESP]Matta, Daniel Archimedes da [UNIFESP]Colombo, Arnaldo Lopes [UNIFESP]2015-06-14T13:30:02Z2015-06-14T13:30:02Z2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion533-538application/pdfhttp://dx.doi.org/10.1590/S0074-02762003000400020Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003.10.1590/S0074-02762003000400020S0074-02762003000400020.pdf0074-0276S0074-02762003000400020http://repositorio.unifesp.br/handle/11600/1745WOS:000183993100020engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T03:05:48Zoai:repositorio.unifesp.br/:11600/1745Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T03:05:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Candida dubliniensis identification in Brazilian yeast stock collection
title Candida dubliniensis identification in Brazilian yeast stock collection
spellingShingle Candida dubliniensis identification in Brazilian yeast stock collection
Mariano, Priscilla de Laet Sant'ana [UNIFESP]
Candida dubliniensis
oral candidiasis
Candida spp.
Aids
title_short Candida dubliniensis identification in Brazilian yeast stock collection
title_full Candida dubliniensis identification in Brazilian yeast stock collection
title_fullStr Candida dubliniensis identification in Brazilian yeast stock collection
title_full_unstemmed Candida dubliniensis identification in Brazilian yeast stock collection
title_sort Candida dubliniensis identification in Brazilian yeast stock collection
author Mariano, Priscilla de Laet Sant'ana [UNIFESP]
author_facet Mariano, Priscilla de Laet Sant'ana [UNIFESP]
Milan, Eveline Pípolo [UNIFESP]
Matta, Daniel Archimedes da [UNIFESP]
Colombo, Arnaldo Lopes [UNIFESP]
author_role author
author2 Milan, Eveline Pípolo [UNIFESP]
Matta, Daniel Archimedes da [UNIFESP]
Colombo, Arnaldo Lopes [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e Parasitologia
dc.contributor.author.fl_str_mv Mariano, Priscilla de Laet Sant'ana [UNIFESP]
Milan, Eveline Pípolo [UNIFESP]
Matta, Daniel Archimedes da [UNIFESP]
Colombo, Arnaldo Lopes [UNIFESP]
dc.subject.por.fl_str_mv Candida dubliniensis
oral candidiasis
Candida spp.
Aids
topic Candida dubliniensis
oral candidiasis
Candida spp.
Aids
description We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture.
publishDate 2003
dc.date.none.fl_str_mv 2003-06-01
2015-06-14T13:30:02Z
2015-06-14T13:30:02Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762003000400020
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003.
10.1590/S0074-02762003000400020
S0074-02762003000400020.pdf
0074-0276
S0074-02762003000400020
http://repositorio.unifesp.br/handle/11600/1745
WOS:000183993100020
url http://dx.doi.org/10.1590/S0074-02762003000400020
http://repositorio.unifesp.br/handle/11600/1745
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 98, n. 4, p. 533-538, 2003.
10.1590/S0074-02762003000400020
S0074-02762003000400020.pdf
0074-0276
S0074-02762003000400020
WOS:000183993100020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 533-538
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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