Apoptosis in the early developing periodontium of rat molars
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/42533 http://dx.doi.org/10.1002/(SICI)1097-0185(20000201)258:2<136 |
Resumo: | Development of the periodontium involves a series of complex steps that result in the formation of root dentine, cementum, bone and fibres of the ligament. These precisely controlled and timed events require the participation of the enamel organ derived epithelial cells of Hertwig's (HRS) and ectomesenchymal cells of the dental follicle. These events involve rapid turnover of the tissues and cells, including disappearance of epithelial cells of HRS, Thus, it seemed likely to us that programmed cell death (apoptosis) may play a role in the development of the periodontium.Fragments of first molars, obtained from 14- and 29-day-old rats, were fixed in glutaraldehyde-formaldehyde and processed for light and electron microscopy. For the TUNEL method for detection of apoptosis, specimens were fixed in 4% formaldehyde and embedded in paraffin.Results confirmed that epithelial cells of HRS maintain a close relationship with the forming dentine root, and that they may become trapped in the dentino-cemental junction. Some of the epithelial cells exhibited ultrastructural features which are consistent with the interpretation that they were undergoing programmed cell death, i.e. apoptosis. Periodontal fibroblast-like cells showed typical images of apoptosis and engulfed apoptotic bodies; TUNEL positive structures were present in all corresponding regions. It seems therefore that apoptosis of epithelial cells of HRS and fibroblast-like cells of the periodontal Ligament constitutes an integral part of the developmental process of the tissues of the periodontium. Anat Rec 258:136-144, 2000. (C) 2000 Wiley-Liss, Inc. |
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Cerri, Paulo Sergio [UNIFESP]Freymüller-Haapalainen, Edna [UNIFESP]Katchburian, Eduardo [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2018-06-15T13:50:09Z2018-06-15T13:50:09Z2000-02-01Anatomical Record. New York: Wiley-liss, v. 258, n. 2, p. 136-144, 2000.0003-276Xhttp://repositorio.unifesp.br/11600/42533http://dx.doi.org/10.1002/(SICI)1097-0185(20000201)258:2<13610.1002/(SICI)1097-0185(20000201)258:2<136WOS:000085120800003Development of the periodontium involves a series of complex steps that result in the formation of root dentine, cementum, bone and fibres of the ligament. These precisely controlled and timed events require the participation of the enamel organ derived epithelial cells of Hertwig's (HRS) and ectomesenchymal cells of the dental follicle. These events involve rapid turnover of the tissues and cells, including disappearance of epithelial cells of HRS, Thus, it seemed likely to us that programmed cell death (apoptosis) may play a role in the development of the periodontium.Fragments of first molars, obtained from 14- and 29-day-old rats, were fixed in glutaraldehyde-formaldehyde and processed for light and electron microscopy. For the TUNEL method for detection of apoptosis, specimens were fixed in 4% formaldehyde and embedded in paraffin.Results confirmed that epithelial cells of HRS maintain a close relationship with the forming dentine root, and that they may become trapped in the dentino-cemental junction. Some of the epithelial cells exhibited ultrastructural features which are consistent with the interpretation that they were undergoing programmed cell death, i.e. apoptosis. Periodontal fibroblast-like cells showed typical images of apoptosis and engulfed apoptotic bodies; TUNEL positive structures were present in all corresponding regions. It seems therefore that apoptosis of epithelial cells of HRS and fibroblast-like cells of the periodontal Ligament constitutes an integral part of the developmental process of the tissues of the periodontium. Anat Rec 258:136-144, 2000. (C) 2000 Wiley-Liss, Inc.Univ Fed Sao Paulo, Sch Med, Dept Morphol, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Ctr Electron Microscopy, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Morphol, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Sch Med, Dept Morphol, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Ctr Electron Microscopy, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Morphol, BR-04023900 Sao Paulo, BrazilWeb of Science136-144engWiley-BlackwellAnatomical Recordhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessapoptosisHertwig's root sheathperiodontal ligamentperiodontiumcementumApoptosis in the early developing periodontium of rat molarsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/425332022-02-08 17:45:33.36metadata only accessoai:repositorio.unifesp.br:11600/42533Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T20:45:33Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Apoptosis in the early developing periodontium of rat molars |
title |
Apoptosis in the early developing periodontium of rat molars |
spellingShingle |
Apoptosis in the early developing periodontium of rat molars Cerri, Paulo Sergio [UNIFESP] apoptosis Hertwig's root sheath periodontal ligament periodontium cementum |
title_short |
Apoptosis in the early developing periodontium of rat molars |
title_full |
Apoptosis in the early developing periodontium of rat molars |
title_fullStr |
Apoptosis in the early developing periodontium of rat molars |
title_full_unstemmed |
Apoptosis in the early developing periodontium of rat molars |
title_sort |
Apoptosis in the early developing periodontium of rat molars |
author |
Cerri, Paulo Sergio [UNIFESP] |
author_facet |
Cerri, Paulo Sergio [UNIFESP] Freymüller-Haapalainen, Edna [UNIFESP] Katchburian, Eduardo [UNIFESP] |
author_role |
author |
author2 |
Freymüller-Haapalainen, Edna [UNIFESP] Katchburian, Eduardo [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Cerri, Paulo Sergio [UNIFESP] Freymüller-Haapalainen, Edna [UNIFESP] Katchburian, Eduardo [UNIFESP] |
dc.subject.eng.fl_str_mv |
apoptosis Hertwig's root sheath periodontal ligament periodontium cementum |
topic |
apoptosis Hertwig's root sheath periodontal ligament periodontium cementum |
description |
Development of the periodontium involves a series of complex steps that result in the formation of root dentine, cementum, bone and fibres of the ligament. These precisely controlled and timed events require the participation of the enamel organ derived epithelial cells of Hertwig's (HRS) and ectomesenchymal cells of the dental follicle. These events involve rapid turnover of the tissues and cells, including disappearance of epithelial cells of HRS, Thus, it seemed likely to us that programmed cell death (apoptosis) may play a role in the development of the periodontium.Fragments of first molars, obtained from 14- and 29-day-old rats, were fixed in glutaraldehyde-formaldehyde and processed for light and electron microscopy. For the TUNEL method for detection of apoptosis, specimens were fixed in 4% formaldehyde and embedded in paraffin.Results confirmed that epithelial cells of HRS maintain a close relationship with the forming dentine root, and that they may become trapped in the dentino-cemental junction. Some of the epithelial cells exhibited ultrastructural features which are consistent with the interpretation that they were undergoing programmed cell death, i.e. apoptosis. Periodontal fibroblast-like cells showed typical images of apoptosis and engulfed apoptotic bodies; TUNEL positive structures were present in all corresponding regions. It seems therefore that apoptosis of epithelial cells of HRS and fibroblast-like cells of the periodontal Ligament constitutes an integral part of the developmental process of the tissues of the periodontium. Anat Rec 258:136-144, 2000. (C) 2000 Wiley-Liss, Inc. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000-02-01 |
dc.date.accessioned.fl_str_mv |
2018-06-15T13:50:09Z |
dc.date.available.fl_str_mv |
2018-06-15T13:50:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Anatomical Record. New York: Wiley-liss, v. 258, n. 2, p. 136-144, 2000. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/42533 http://dx.doi.org/10.1002/(SICI)1097-0185(20000201)258:2<136 |
dc.identifier.issn.none.fl_str_mv |
0003-276X |
dc.identifier.doi.none.fl_str_mv |
10.1002/(SICI)1097-0185(20000201)258:2<136 |
dc.identifier.wos.none.fl_str_mv |
WOS:000085120800003 |
identifier_str_mv |
Anatomical Record. New York: Wiley-liss, v. 258, n. 2, p. 136-144, 2000. 0003-276X 10.1002/(SICI)1097-0185(20000201)258:2<136 WOS:000085120800003 |
url |
http://repositorio.unifesp.br/11600/42533 http://dx.doi.org/10.1002/(SICI)1097-0185(20000201)258:2<136 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Anatomical Record |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
136-144 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764172053708800 |