Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1590/S0004-27302013000500006 https://repositorio.unifesp.br/handle/11600/7854 |
Resumo: | OBJECTIVE: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction. RESULTS: F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group. CONCLUSIONS: T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes. |
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Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different timesModulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes temposTRαTRβTriiodothyronineAdipocytesTRαTRβTriiodotironinaAdipócitosOBJECTIVE: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction. RESULTS: F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group. CONCLUSIONS: T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.OBJETIVO: Examinar o efeito de diferentes doses de triiodotironina (T3) sobre a expressão gênica dos receptores TRα e TRβ em diferentes tempos. MATERIAIS E MÉTODOS: Adipócitos, 3T3-L1, foram incubados com T3 nas doses fisiológica (F, 10nM) e suprafisiológicas (SI, 100nM ou SII, 1000nM) ou veículo (controle, C) durante 0,5, 1, 6 ou 24h. mRNA dos TRs foram detectados utilizando PCR em tempo real. RESULTADOS: Níveis de TRβ aumentaram em F em 0,5h. Após 1h, níveis de TRα aumentaram em F e SI comparado ao C, enquanto TRβ diminuiu no SII comparado com C, F, e SI. Após 6h, ambos os genes foram suprimidos em todas concentrações. Em 24h, níveis de TRα e TRβ retornaram aos do C. CONCLUSÕES: Ação do T3 em F iniciou-se em 1h para TRα e 0,5h para TRβ. Esses resultados são importantes para determinar tempo inicial e dose de T3 em que os receptores de HT são ativados em adipócitos.Unesp Botucatu Medicine School Department of Internal ClinicUniversidade Federal de São Paulo (UNIFESP) Department of PhysiologyUnesp Biosciences Institute Department of BiostatisticsUNIFESP, Department of PhysiologySciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sociedade Brasileira de Endocrinologia e MetabologiaUniversidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Oliveira, Miriane deLuvizotto, Renata de Azevedo MeloOlimpio, Regiane Marques CastroSibio, Maria Teresa deBiz, Carolina [UNIFESP]Conde, Sandro JoséPadovani, Carlos RobertoNogueira, Célia Regina2015-06-14T13:45:32Z2015-06-14T13:45:32Z2013-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion368-374application/pdfhttps://dx.doi.org/10.1590/S0004-27302013000500006Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 57, n. 5, p. 368-374, 2013.10.1590/S0004-27302013000500006S0004-27302013000500006.pdf0004-2730S0004-27302013000500006https://repositorio.unifesp.br/handle/11600/7854WOS:000322398100006engArquivos Brasileiros de Endocrinologia & Metabologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T07:05:32Zoai:repositorio.unifesp.br/:11600/7854Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T07:05:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times Modulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes tempos |
title |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times |
spellingShingle |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times Oliveira, Miriane de TRα TRβ Triiodothyronine Adipocytes TRα TRβ Triiodotironina Adipócitos |
title_short |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times |
title_full |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times |
title_fullStr |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times |
title_full_unstemmed |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times |
title_sort |
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times |
author |
Oliveira, Miriane de |
author_facet |
Oliveira, Miriane de Luvizotto, Renata de Azevedo Melo Olimpio, Regiane Marques Castro Sibio, Maria Teresa de Biz, Carolina [UNIFESP] Conde, Sandro José Padovani, Carlos Roberto Nogueira, Célia Regina |
author_role |
author |
author2 |
Luvizotto, Renata de Azevedo Melo Olimpio, Regiane Marques Castro Sibio, Maria Teresa de Biz, Carolina [UNIFESP] Conde, Sandro José Padovani, Carlos Roberto Nogueira, Célia Regina |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Miriane de Luvizotto, Renata de Azevedo Melo Olimpio, Regiane Marques Castro Sibio, Maria Teresa de Biz, Carolina [UNIFESP] Conde, Sandro José Padovani, Carlos Roberto Nogueira, Célia Regina |
dc.subject.por.fl_str_mv |
TRα TRβ Triiodothyronine Adipocytes TRα TRβ Triiodotironina Adipócitos |
topic |
TRα TRβ Triiodothyronine Adipocytes TRα TRβ Triiodotironina Adipócitos |
description |
OBJECTIVE: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction. RESULTS: F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group. CONCLUSIONS: T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01 2015-06-14T13:45:32Z 2015-06-14T13:45:32Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1590/S0004-27302013000500006 Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 57, n. 5, p. 368-374, 2013. 10.1590/S0004-27302013000500006 S0004-27302013000500006.pdf 0004-2730 S0004-27302013000500006 https://repositorio.unifesp.br/handle/11600/7854 WOS:000322398100006 |
url |
https://dx.doi.org/10.1590/S0004-27302013000500006 https://repositorio.unifesp.br/handle/11600/7854 |
identifier_str_mv |
Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 57, n. 5, p. 368-374, 2013. 10.1590/S0004-27302013000500006 S0004-27302013000500006.pdf 0004-2730 S0004-27302013000500006 WOS:000322398100006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Endocrinologia & Metabologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
368-374 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Endocrinologia e Metabologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Endocrinologia e Metabologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268451856842752 |