Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times

Detalhes bibliográficos
Autor(a) principal: Oliveira, Miriane de
Data de Publicação: 2013
Outros Autores: Luvizotto, Renata de Azevedo Melo, Olimpio, Regiane Marques Castro, Sibio, Maria Teresa de, Biz, Carolina [UNIFESP], Conde, Sandro José, Padovani, Carlos Roberto, Nogueira, Célia Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1590/S0004-27302013000500006
https://repositorio.unifesp.br/handle/11600/7854
Resumo: OBJECTIVE: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction. RESULTS: F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group. CONCLUSIONS: T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
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spelling Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different timesModulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes temposTRαTRβTriiodothyronineAdipocytesTRαTRβTriiodotironinaAdipócitosOBJECTIVE: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction. RESULTS: F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group. CONCLUSIONS: T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.OBJETIVO: Examinar o efeito de diferentes doses de triiodotironina (T3) sobre a expressão gênica dos receptores TRα e TRβ em diferentes tempos. MATERIAIS E MÉTODOS: Adipócitos, 3T3-L1, foram incubados com T3 nas doses fisiológica (F, 10nM) e suprafisiológicas (SI, 100nM ou SII, 1000nM) ou veículo (controle, C) durante 0,5, 1, 6 ou 24h. mRNA dos TRs foram detectados utilizando PCR em tempo real. RESULTADOS: Níveis de TRβ aumentaram em F em 0,5h. Após 1h, níveis de TRα aumentaram em F e SI comparado ao C, enquanto TRβ diminuiu no SII comparado com C, F, e SI. Após 6h, ambos os genes foram suprimidos em todas concentrações. Em 24h, níveis de TRα e TRβ retornaram aos do C. CONCLUSÕES: Ação do T3 em F iniciou-se em 1h para TRα e 0,5h para TRβ. Esses resultados são importantes para determinar tempo inicial e dose de T3 em que os receptores de HT são ativados em adipócitos.Unesp Botucatu Medicine School Department of Internal ClinicUniversidade Federal de São Paulo (UNIFESP) Department of PhysiologyUnesp Biosciences Institute Department of BiostatisticsUNIFESP, Department of PhysiologySciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sociedade Brasileira de Endocrinologia e MetabologiaUniversidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Oliveira, Miriane deLuvizotto, Renata de Azevedo MeloOlimpio, Regiane Marques CastroSibio, Maria Teresa deBiz, Carolina [UNIFESP]Conde, Sandro JoséPadovani, Carlos RobertoNogueira, Célia Regina2015-06-14T13:45:32Z2015-06-14T13:45:32Z2013-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion368-374application/pdfhttps://dx.doi.org/10.1590/S0004-27302013000500006Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 57, n. 5, p. 368-374, 2013.10.1590/S0004-27302013000500006S0004-27302013000500006.pdf0004-2730S0004-27302013000500006https://repositorio.unifesp.br/handle/11600/7854WOS:000322398100006engArquivos Brasileiros de Endocrinologia & Metabologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T07:05:32Zoai:repositorio.unifesp.br/:11600/7854Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T07:05:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
Modulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes tempos
title Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
spellingShingle Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
Oliveira, Miriane de
TRα
TRβ
Triiodothyronine
Adipocytes
TRα
TRβ
Triiodotironina
Adipócitos
title_short Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
title_full Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
title_fullStr Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
title_full_unstemmed Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
title_sort Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times
author Oliveira, Miriane de
author_facet Oliveira, Miriane de
Luvizotto, Renata de Azevedo Melo
Olimpio, Regiane Marques Castro
Sibio, Maria Teresa de
Biz, Carolina [UNIFESP]
Conde, Sandro José
Padovani, Carlos Roberto
Nogueira, Célia Regina
author_role author
author2 Luvizotto, Renata de Azevedo Melo
Olimpio, Regiane Marques Castro
Sibio, Maria Teresa de
Biz, Carolina [UNIFESP]
Conde, Sandro José
Padovani, Carlos Roberto
Nogueira, Célia Regina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Oliveira, Miriane de
Luvizotto, Renata de Azevedo Melo
Olimpio, Regiane Marques Castro
Sibio, Maria Teresa de
Biz, Carolina [UNIFESP]
Conde, Sandro José
Padovani, Carlos Roberto
Nogueira, Célia Regina
dc.subject.por.fl_str_mv TRα
TRβ
Triiodothyronine
Adipocytes
TRα
TRβ
Triiodotironina
Adipócitos
topic TRα
TRβ
Triiodothyronine
Adipocytes
TRα
TRβ
Triiodotironina
Adipócitos
description OBJECTIVE: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS AND METHODS: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction. RESULTS: F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group. CONCLUSIONS: T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-01
2015-06-14T13:45:32Z
2015-06-14T13:45:32Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1590/S0004-27302013000500006
Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 57, n. 5, p. 368-374, 2013.
10.1590/S0004-27302013000500006
S0004-27302013000500006.pdf
0004-2730
S0004-27302013000500006
https://repositorio.unifesp.br/handle/11600/7854
WOS:000322398100006
url https://dx.doi.org/10.1590/S0004-27302013000500006
https://repositorio.unifesp.br/handle/11600/7854
identifier_str_mv Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 57, n. 5, p. 368-374, 2013.
10.1590/S0004-27302013000500006
S0004-27302013000500006.pdf
0004-2730
S0004-27302013000500006
WOS:000322398100006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Endocrinologia & Metabologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 368-374
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Endocrinologia e Metabologia
publisher.none.fl_str_mv Sociedade Brasileira de Endocrinologia e Metabologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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