Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times

Detalhes bibliográficos
Autor(a) principal: Oliveira, Miriane de [UNESP]
Data de Publicação: 2013
Outros Autores: Melo Luvizotto, Renata de Azevedo [UNESP], Castro Olimpio, Regiane Marques [UNESP], Sibio, Maria Teresa de [UNESP], Rodrigues Silva, Carolina Biz, Conde, Sandro Jose [UNESP], Padovani, Carlos Roberto [UNESP], Nogueira, Celia Regina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0004-27302013000500006
http://hdl.handle.net/11449/132408
Resumo: Objective: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TR alpha and TR beta, at different times. Materials and methods: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TR alpha and TR beta mRNA was detected using real-time polymerase chain reaction. Results: F increased TR beta mRNA levels at 0.5h. After 1h, TR alpha levels increased with F and SI and TR beta levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TR alpha and TR beta levels were similar to those of C group. Conclusions: T3 action with F began at 1h for TR alpha and at 0.5h for TR beta. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
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spelling Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different timesModulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes temposTR alphaTR betatriiodothyronineadipocytesObjective: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TR alpha and TR beta, at different times. Materials and methods: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TR alpha and TR beta mRNA was detected using real-time polymerase chain reaction. Results: F increased TR beta mRNA levels at 0.5h. After 1h, TR alpha levels increased with F and SI and TR beta levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TR alpha and TR beta levels were similar to those of C group. Conclusions: T3 action with F began at 1h for TR alpha and at 0.5h for TR beta. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ Unesp, Botucatu Med Sch, Dept Internal Clin, Botucatu, SP, BrazilSao Paulo Fed Univ Unifesp, Dept Physiol, Sao Paulo, BrazilUNESP, Biosci Inst, Dept Biostat, Botucatu, SP, BrazilSao Paulo State Univ Unesp, Botucatu Med Sch, Dept Internal Clin, Botucatu, SP, BrazilUNESP, Biosci Inst, Dept Biostat, Botucatu, SP, BrazilFAPESP: 10/16911-4Sbem-soc Brasil Endocrinologia & MetabologiaUniversidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Oliveira, Miriane de [UNESP]Melo Luvizotto, Renata de Azevedo [UNESP]Castro Olimpio, Regiane Marques [UNESP]Sibio, Maria Teresa de [UNESP]Rodrigues Silva, Carolina BizConde, Sandro Jose [UNESP]Padovani, Carlos Roberto [UNESP]Nogueira, Celia Regina [UNESP]2014-12-03T13:10:51Z2014-12-03T13:10:51Z2013-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-374application/pdfhttp://dx.doi.org/10.1590/S0004-27302013000500006Arquivos Brasileiros de Endocrinologia e Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 57, n. 5, p. 368-374, 2013.0004-27301677-9487http://hdl.handle.net/11449/13240810.1590/S0004-27302013000500006S0004-27302013000500006WOS:0003223981000062-s2.0-84880955870S0004-27302013000500006.pdf8727897080522289Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Endocrinologia & Metabologia1.515info:eu-repo/semantics/openAccess2023-12-06T06:13:38Zoai:repositorio.unesp.br:11449/132408Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:35:18.419877Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
Modulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes tempos
title Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
spellingShingle Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
Oliveira, Miriane de [UNESP]
TR alpha
TR beta
triiodothyronine
adipocytes
title_short Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
title_full Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
title_fullStr Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
title_full_unstemmed Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
title_sort Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
author Oliveira, Miriane de [UNESP]
author_facet Oliveira, Miriane de [UNESP]
Melo Luvizotto, Renata de Azevedo [UNESP]
Castro Olimpio, Regiane Marques [UNESP]
Sibio, Maria Teresa de [UNESP]
Rodrigues Silva, Carolina Biz
Conde, Sandro Jose [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Celia Regina [UNESP]
author_role author
author2 Melo Luvizotto, Renata de Azevedo [UNESP]
Castro Olimpio, Regiane Marques [UNESP]
Sibio, Maria Teresa de [UNESP]
Rodrigues Silva, Carolina Biz
Conde, Sandro Jose [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Celia Regina [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Oliveira, Miriane de [UNESP]
Melo Luvizotto, Renata de Azevedo [UNESP]
Castro Olimpio, Regiane Marques [UNESP]
Sibio, Maria Teresa de [UNESP]
Rodrigues Silva, Carolina Biz
Conde, Sandro Jose [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Celia Regina [UNESP]
dc.subject.por.fl_str_mv TR alpha
TR beta
triiodothyronine
adipocytes
topic TR alpha
TR beta
triiodothyronine
adipocytes
description Objective: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TR alpha and TR beta, at different times. Materials and methods: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TR alpha and TR beta mRNA was detected using real-time polymerase chain reaction. Results: F increased TR beta mRNA levels at 0.5h. After 1h, TR alpha levels increased with F and SI and TR beta levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TR alpha and TR beta levels were similar to those of C group. Conclusions: T3 action with F began at 1h for TR alpha and at 0.5h for TR beta. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-01
2014-12-03T13:10:51Z
2014-12-03T13:10:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-27302013000500006
Arquivos Brasileiros de Endocrinologia e Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 57, n. 5, p. 368-374, 2013.
0004-2730
1677-9487
http://hdl.handle.net/11449/132408
10.1590/S0004-27302013000500006
S0004-27302013000500006
WOS:000322398100006
2-s2.0-84880955870
S0004-27302013000500006.pdf
8727897080522289
url http://dx.doi.org/10.1590/S0004-27302013000500006
http://hdl.handle.net/11449/132408
identifier_str_mv Arquivos Brasileiros de Endocrinologia e Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 57, n. 5, p. 368-374, 2013.
0004-2730
1677-9487
10.1590/S0004-27302013000500006
S0004-27302013000500006
WOS:000322398100006
2-s2.0-84880955870
S0004-27302013000500006.pdf
8727897080522289
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Endocrinologia & Metabologia
1.515
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 368-374
application/pdf
dc.publisher.none.fl_str_mv Sbem-soc Brasil Endocrinologia & Metabologia
publisher.none.fl_str_mv Sbem-soc Brasil Endocrinologia & Metabologia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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