Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0004-27302013000500006 http://hdl.handle.net/11449/132408 |
Resumo: | Objective: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TR alpha and TR beta, at different times. Materials and methods: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TR alpha and TR beta mRNA was detected using real-time polymerase chain reaction. Results: F increased TR beta mRNA levels at 0.5h. After 1h, TR alpha levels increased with F and SI and TR beta levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TR alpha and TR beta levels were similar to those of C group. Conclusions: T3 action with F began at 1h for TR alpha and at 0.5h for TR beta. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes. |
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Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different timesModulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes temposTR alphaTR betatriiodothyronineadipocytesObjective: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TR alpha and TR beta, at different times. Materials and methods: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TR alpha and TR beta mRNA was detected using real-time polymerase chain reaction. Results: F increased TR beta mRNA levels at 0.5h. After 1h, TR alpha levels increased with F and SI and TR beta levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TR alpha and TR beta levels were similar to those of C group. Conclusions: T3 action with F began at 1h for TR alpha and at 0.5h for TR beta. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ Unesp, Botucatu Med Sch, Dept Internal Clin, Botucatu, SP, BrazilSao Paulo Fed Univ Unifesp, Dept Physiol, Sao Paulo, BrazilUNESP, Biosci Inst, Dept Biostat, Botucatu, SP, BrazilSao Paulo State Univ Unesp, Botucatu Med Sch, Dept Internal Clin, Botucatu, SP, BrazilUNESP, Biosci Inst, Dept Biostat, Botucatu, SP, BrazilFAPESP: 10/16911-4Sbem-soc Brasil Endocrinologia & MetabologiaUniversidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Oliveira, Miriane de [UNESP]Melo Luvizotto, Renata de Azevedo [UNESP]Castro Olimpio, Regiane Marques [UNESP]Sibio, Maria Teresa de [UNESP]Rodrigues Silva, Carolina BizConde, Sandro Jose [UNESP]Padovani, Carlos Roberto [UNESP]Nogueira, Celia Regina [UNESP]2014-12-03T13:10:51Z2014-12-03T13:10:51Z2013-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-374application/pdfhttp://dx.doi.org/10.1590/S0004-27302013000500006Arquivos Brasileiros de Endocrinologia e Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 57, n. 5, p. 368-374, 2013.0004-27301677-9487http://hdl.handle.net/11449/13240810.1590/S0004-27302013000500006S0004-27302013000500006WOS:0003223981000062-s2.0-84880955870S0004-27302013000500006.pdf8727897080522289Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Endocrinologia & Metabologia1.515info:eu-repo/semantics/openAccess2023-12-06T06:13:38Zoai:repositorio.unesp.br:11449/132408Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:35:18.419877Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times Modulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes tempos |
title |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times |
spellingShingle |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times Oliveira, Miriane de [UNESP] TR alpha TR beta triiodothyronine adipocytes |
title_short |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times |
title_full |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times |
title_fullStr |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times |
title_full_unstemmed |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times |
title_sort |
Modulation of thyroid hormone receptors, TR alpha and TR beta, by using different doses of triiodothyronine (T3) at different times |
author |
Oliveira, Miriane de [UNESP] |
author_facet |
Oliveira, Miriane de [UNESP] Melo Luvizotto, Renata de Azevedo [UNESP] Castro Olimpio, Regiane Marques [UNESP] Sibio, Maria Teresa de [UNESP] Rodrigues Silva, Carolina Biz Conde, Sandro Jose [UNESP] Padovani, Carlos Roberto [UNESP] Nogueira, Celia Regina [UNESP] |
author_role |
author |
author2 |
Melo Luvizotto, Renata de Azevedo [UNESP] Castro Olimpio, Regiane Marques [UNESP] Sibio, Maria Teresa de [UNESP] Rodrigues Silva, Carolina Biz Conde, Sandro Jose [UNESP] Padovani, Carlos Roberto [UNESP] Nogueira, Celia Regina [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Miriane de [UNESP] Melo Luvizotto, Renata de Azevedo [UNESP] Castro Olimpio, Regiane Marques [UNESP] Sibio, Maria Teresa de [UNESP] Rodrigues Silva, Carolina Biz Conde, Sandro Jose [UNESP] Padovani, Carlos Roberto [UNESP] Nogueira, Celia Regina [UNESP] |
dc.subject.por.fl_str_mv |
TR alpha TR beta triiodothyronine adipocytes |
topic |
TR alpha TR beta triiodothyronine adipocytes |
description |
Objective: To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TR alpha and TR beta, at different times. Materials and methods: 3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TR alpha and TR beta mRNA was detected using real-time polymerase chain reaction. Results: F increased TR beta mRNA levels at 0.5h. After 1h, TR alpha levels increased with F and SI and TR beta levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TR alpha and TR beta levels were similar to those of C group. Conclusions: T3 action with F began at 1h for TR alpha and at 0.5h for TR beta. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01 2014-12-03T13:10:51Z 2014-12-03T13:10:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0004-27302013000500006 Arquivos Brasileiros de Endocrinologia e Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 57, n. 5, p. 368-374, 2013. 0004-2730 1677-9487 http://hdl.handle.net/11449/132408 10.1590/S0004-27302013000500006 S0004-27302013000500006 WOS:000322398100006 2-s2.0-84880955870 S0004-27302013000500006.pdf 8727897080522289 |
url |
http://dx.doi.org/10.1590/S0004-27302013000500006 http://hdl.handle.net/11449/132408 |
identifier_str_mv |
Arquivos Brasileiros de Endocrinologia e Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 57, n. 5, p. 368-374, 2013. 0004-2730 1677-9487 10.1590/S0004-27302013000500006 S0004-27302013000500006 WOS:000322398100006 2-s2.0-84880955870 S0004-27302013000500006.pdf 8727897080522289 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Endocrinologia & Metabologia 1.515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
368-374 application/pdf |
dc.publisher.none.fl_str_mv |
Sbem-soc Brasil Endocrinologia & Metabologia |
publisher.none.fl_str_mv |
Sbem-soc Brasil Endocrinologia & Metabologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129090904915968 |