Validation of a new fiber electrode prototype for clinical electroretinography

Detalhes bibliográficos
Autor(a) principal: Berezovsky, Adriana [UNIFESP]
Data de Publicação: 2008
Outros Autores: Pereira, Josenilson Martins [UNIFESP], Salomão, Solange Rios [UNIFESP], Santos, Vagner Rogério Dos [UNIFESP], Schor, Paulo [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0004-27492008000300003
http://repositorio.unifesp.br/handle/11600/4380
Resumo: PURPOSE: To validate a new fiber electrode prototype for clinical electroretinography (ERG). METHODS: A recently developed prototype of a disposable reference-coupled fiber electrode (patent pending Brazilian Institute of Industrial Property # PI0602186-7), including one fiber for corneal signals and a second fiber acting as reference was tested in a group of 20 healthy volunteers (17-31 years; mean 22.7 ± 4.5; 8 males). Standard electroretinography rod and cone responses were recorded from a fully dilated pupil simultaneously in both eyes with a reference-coupled fiber electrode prototype in one randomly assigned eye and a DTL® electrode in the other eye after 30 min of dark-adaptation. After presenting dark- and light-adapted stimuli, each response was analyzed for a- and b-wave amplitude and implicit time. The VERIS 5.1.9 system was used for electroretinography data acquisition and analysis. Electroretinography outcomes were analyzed by Mann-Whitney test. Slit-lamp examination was performed in both eyes right after electroretinography session to evaluate possible adverse effects. RESULTS: Responses recorded with reference-coupled fiber electrode prototypes were comparable to commercially available DTL® fiber electrodes. On a qualitative analysis, reference-coupled fiber electrodes provided recordings with less amount of noise. On average, scotopic electroretinography amplitude and b-wave implicit time recorded using DTL® were, respectively, 287.6 µV and 36.3 ms with similar findings for the reference-coupled fiber electrode prototype (287.9 µV and 36.3 ms). Under photopic conditions DTL® mean amplitude and implicit time were, respectively 108.9 µV and 24.5 ms with similar results for the reference-coupled fiber electrodes prototypes (116.4 µV and 24.5 ms). No corneal abrasions or any other significant adverse effects were found after electroretinography recording with both electrodes. CONCLUSIONS: The reference-coupled fiber electrode prototype provided stable and safe recordings of corneal electroretinograms compared to the commercially availabe DTL® electrode in healthy human subjects. The prototype is a feasible alternative instrument for clinical electroretinography recording to assess retinal function, however further analysis is recommended to validate its clinical usefulness in patients with retinal disorders.
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spelling Validation of a new fiber electrode prototype for clinical electroretinographyValidação de protótipo de um novo eletrodo de fibra para eletrorretinografia clínicaElectrodesElectroretinographyRetinaRetinal diseasesValidation studiesEletrodosEletrorretinografiaRetinaDoenças retinianasEstudos de validaçãoPURPOSE: To validate a new fiber electrode prototype for clinical electroretinography (ERG). METHODS: A recently developed prototype of a disposable reference-coupled fiber electrode (patent pending Brazilian Institute of Industrial Property # PI0602186-7), including one fiber for corneal signals and a second fiber acting as reference was tested in a group of 20 healthy volunteers (17-31 years; mean 22.7 ± 4.5; 8 males). Standard electroretinography rod and cone responses were recorded from a fully dilated pupil simultaneously in both eyes with a reference-coupled fiber electrode prototype in one randomly assigned eye and a DTL® electrode in the other eye after 30 min of dark-adaptation. After presenting dark- and light-adapted stimuli, each response was analyzed for a- and b-wave amplitude and implicit time. The VERIS 5.1.9 system was used for electroretinography data acquisition and analysis. Electroretinography outcomes were analyzed by Mann-Whitney test. Slit-lamp examination was performed in both eyes right after electroretinography session to evaluate possible adverse effects. RESULTS: Responses recorded with reference-coupled fiber electrode prototypes were comparable to commercially available DTL® fiber electrodes. On a qualitative analysis, reference-coupled fiber electrodes provided recordings with less amount of noise. On average, scotopic electroretinography amplitude and b-wave implicit time recorded using DTL® were, respectively, 287.6 µV and 36.3 ms with similar findings for the reference-coupled fiber electrode prototype (287.9 µV and 36.3 ms). Under photopic conditions DTL® mean amplitude and implicit time were, respectively 108.9 µV and 24.5 ms with similar results for the reference-coupled fiber electrodes prototypes (116.4 µV and 24.5 ms). No corneal abrasions or any other significant adverse effects were found after electroretinography recording with both electrodes. CONCLUSIONS: The reference-coupled fiber electrode prototype provided stable and safe recordings of corneal electroretinograms compared to the commercially availabe DTL® electrode in healthy human subjects. The prototype is a feasible alternative instrument for clinical electroretinography recording to assess retinal function, however further analysis is recommended to validate its clinical usefulness in patients with retinal disorders.OBJETIVO: Validar um novo protótipo de um eletrodo de fibra para eletrorretinografia clínica (ERG). MÉTODOS: Foi testado em um grupo de 20 voluntários saudáveis (17-31 anos; média 22,7 ± 4,5; 8 homens), um protótipo de eletrodo de fibra com referência acoplado descartável recentemente desenvolvido (depósito de patente no Instituto Nacional de Propriedade Intelectual # PI0602186-7), constando de uma fibra de sinais corneanos e uma segunda fibra servindo como referência. Após 30 minutos de adaptação ao escuro, as respostas de cones e bastonetes da eletrorretinografia foram registradas simultaneamente com dilatação completa das pupilas em ambos os olhos, com o protótipo de eletrodo de fibra com referência acoplado em um olho escolhido ao acaso e um eletrodo DTL® no outro olho. Após a apresentação dos estímulos escotópicos e fotópicos cada resposta foi analisada em amplitude e tempo de culminação das ondas a e b. O sistema VERIS 5.1.9 foi usado para a aquisição e análise dos dados. Os resultados da eletrorretinografia foram analisados pelo teste de Mann-Whitney. Após a sessão da eletrorretinografia foi feito exame em lâmpada de fenda para avaliar possíveis eventos adversos. RESULTADOS: As respostas obtidas com o protótipo de eletrodo de fibra com referência acoplado foram comparáveis às do eletrodo DTL® comercialmente disponível. Numa análise qualitativa, o eletrodo de fibra com referência acoplado produziu sinais com menos ruído. Na média, a amplitude escotópica da eletrorretinografia e o tempo de culminação da onda-b usando o eletrodo DTL® foram respectivamente, 287,6 µV and 36,3 ms com achados similares para o protótipo (287,9 µV e 36,3 ms). Sob condições fotópicas, a amplitude média do eletrodo DTL® e o tempo de culminação da onda b foram respectivamente 108,9 µV e 24,5 ms com resultados similares para o protótipo (116,4 µV e 24,5 ms). Após os registros da eletrorretinografia com ambos os tipos de eletrodos não houve abrasões corneanas ou outros eventos adversos significantes. CONCLUSÕES: Em sujeitos humanos saudáveis, o protótipo de eletrodo de fibra com referência acoplado forneceu registros estáveis e seguros de eletrorretinogramas a partir da córnea comparado aos obtidos com o eletrodo DTL® comercialmente disponível. O protótipo é um instrumento alternativo viável para registro clínico da eletrorretinografia para avaliar a função retiniana, no entanto, análises adicionais são necessárias para validar sua utilidade clínica em pacientes com distúrbios retinianos.Universidade Federal de São Paulo (UNIFESP) Departamento de OftalmologiaUNIFESP Departamento de OftalmologiaFaculdade de Tecnologia de São PauloUNIFESPUNIFESP, Depto. de OftalmologiaUNIFESP, Depto. de OftalmologiaUNIFESPSciELOConselho Brasileiro de OftalmologiaUniversidade Federal de São Paulo (UNIFESP)Faculdade de Tecnologia de São PauloBerezovsky, Adriana [UNIFESP]Pereira, Josenilson Martins [UNIFESP]Salomão, Solange Rios [UNIFESP]Santos, Vagner Rogério Dos [UNIFESP]Schor, Paulo [UNIFESP]2015-06-14T13:38:33Z2015-06-14T13:38:33Z2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion316-320application/pdfhttp://dx.doi.org/10.1590/S0004-27492008000300003Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 71, n. 3, p. 316-320, 2008.10.1590/S0004-27492008000300003S0004-27492008000300003.pdf0004-2749S0004-27492008000300003http://repositorio.unifesp.br/handle/11600/4380engArquivos Brasileiros de Oftalmologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T20:09:47Zoai:repositorio.unifesp.br/:11600/4380Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T20:09:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Validation of a new fiber electrode prototype for clinical electroretinography
Validação de protótipo de um novo eletrodo de fibra para eletrorretinografia clínica
title Validation of a new fiber electrode prototype for clinical electroretinography
spellingShingle Validation of a new fiber electrode prototype for clinical electroretinography
Berezovsky, Adriana [UNIFESP]
Electrodes
Electroretinography
Retina
Retinal diseases
Validation studies
Eletrodos
Eletrorretinografia
Retina
Doenças retinianas
Estudos de validação
title_short Validation of a new fiber electrode prototype for clinical electroretinography
title_full Validation of a new fiber electrode prototype for clinical electroretinography
title_fullStr Validation of a new fiber electrode prototype for clinical electroretinography
title_full_unstemmed Validation of a new fiber electrode prototype for clinical electroretinography
title_sort Validation of a new fiber electrode prototype for clinical electroretinography
author Berezovsky, Adriana [UNIFESP]
author_facet Berezovsky, Adriana [UNIFESP]
Pereira, Josenilson Martins [UNIFESP]
Salomão, Solange Rios [UNIFESP]
Santos, Vagner Rogério Dos [UNIFESP]
Schor, Paulo [UNIFESP]
author_role author
author2 Pereira, Josenilson Martins [UNIFESP]
Salomão, Solange Rios [UNIFESP]
Santos, Vagner Rogério Dos [UNIFESP]
Schor, Paulo [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Faculdade de Tecnologia de São Paulo
dc.contributor.author.fl_str_mv Berezovsky, Adriana [UNIFESP]
Pereira, Josenilson Martins [UNIFESP]
Salomão, Solange Rios [UNIFESP]
Santos, Vagner Rogério Dos [UNIFESP]
Schor, Paulo [UNIFESP]
dc.subject.por.fl_str_mv Electrodes
Electroretinography
Retina
Retinal diseases
Validation studies
Eletrodos
Eletrorretinografia
Retina
Doenças retinianas
Estudos de validação
topic Electrodes
Electroretinography
Retina
Retinal diseases
Validation studies
Eletrodos
Eletrorretinografia
Retina
Doenças retinianas
Estudos de validação
description PURPOSE: To validate a new fiber electrode prototype for clinical electroretinography (ERG). METHODS: A recently developed prototype of a disposable reference-coupled fiber electrode (patent pending Brazilian Institute of Industrial Property # PI0602186-7), including one fiber for corneal signals and a second fiber acting as reference was tested in a group of 20 healthy volunteers (17-31 years; mean 22.7 ± 4.5; 8 males). Standard electroretinography rod and cone responses were recorded from a fully dilated pupil simultaneously in both eyes with a reference-coupled fiber electrode prototype in one randomly assigned eye and a DTL® electrode in the other eye after 30 min of dark-adaptation. After presenting dark- and light-adapted stimuli, each response was analyzed for a- and b-wave amplitude and implicit time. The VERIS 5.1.9 system was used for electroretinography data acquisition and analysis. Electroretinography outcomes were analyzed by Mann-Whitney test. Slit-lamp examination was performed in both eyes right after electroretinography session to evaluate possible adverse effects. RESULTS: Responses recorded with reference-coupled fiber electrode prototypes were comparable to commercially available DTL® fiber electrodes. On a qualitative analysis, reference-coupled fiber electrodes provided recordings with less amount of noise. On average, scotopic electroretinography amplitude and b-wave implicit time recorded using DTL® were, respectively, 287.6 µV and 36.3 ms with similar findings for the reference-coupled fiber electrode prototype (287.9 µV and 36.3 ms). Under photopic conditions DTL® mean amplitude and implicit time were, respectively 108.9 µV and 24.5 ms with similar results for the reference-coupled fiber electrodes prototypes (116.4 µV and 24.5 ms). No corneal abrasions or any other significant adverse effects were found after electroretinography recording with both electrodes. CONCLUSIONS: The reference-coupled fiber electrode prototype provided stable and safe recordings of corneal electroretinograms compared to the commercially availabe DTL® electrode in healthy human subjects. The prototype is a feasible alternative instrument for clinical electroretinography recording to assess retinal function, however further analysis is recommended to validate its clinical usefulness in patients with retinal disorders.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-01
2015-06-14T13:38:33Z
2015-06-14T13:38:33Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-27492008000300003
Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 71, n. 3, p. 316-320, 2008.
10.1590/S0004-27492008000300003
S0004-27492008000300003.pdf
0004-2749
S0004-27492008000300003
http://repositorio.unifesp.br/handle/11600/4380
url http://dx.doi.org/10.1590/S0004-27492008000300003
http://repositorio.unifesp.br/handle/11600/4380
identifier_str_mv Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 71, n. 3, p. 316-320, 2008.
10.1590/S0004-27492008000300003
S0004-27492008000300003.pdf
0004-2749
S0004-27492008000300003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Oftalmologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 316-320
application/pdf
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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