BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| dARK ID: | ark:/48912/001300000hqcv |
| Texto Completo: | http://dx.doi.org/10.3389/fmicb.2018.00553 https://repositorio.unifesp.br/handle/11600/55802 |
Resumo: | Trypanosoma cruzi is the etiologic agent of Chagas' disease, which affects 6-7 million people worldwide. Different strains of T. cruzi present specific genotypic and phenotypic characteristics that affect the host-pathogen interactions, and thus, the parasite has been classified into six groups (TcI to TcVI). T. cruzi infection presents two clinical phases, acute and chronic, both with distinct characteristics and important participation by the immune system. However, the specific contributions of parasite and host factors in the disease phases are not yet fully understood. The murine model for Chagas' disease is well-established and reproduces important features of the human infection, providing an experimental basis for the study of host lineages and parasite strains. Thus, we evaluated acute and chronic infection by the G (TcI) and CL (TcVI) strains of T. cruzi, which have distinct tropisms and infectivity, in two inbred mice lineages (C57BL/6 and BALB/c) that display variable degrees of susceptibility to different T. cruzi strains. Analysis of the parasite loads in host tissues by qPCR showed that CL strain established an infection faster than the G strain |
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BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain InfectivityChagas' diseaseTrypanosoma cruziimmune responsecytokinesmiceTrypanosoma cruzi is the etiologic agent of Chagas' disease, which affects 6-7 million people worldwide. Different strains of T. cruzi present specific genotypic and phenotypic characteristics that affect the host-pathogen interactions, and thus, the parasite has been classified into six groups (TcI to TcVI). T. cruzi infection presents two clinical phases, acute and chronic, both with distinct characteristics and important participation by the immune system. However, the specific contributions of parasite and host factors in the disease phases are not yet fully understood. The murine model for Chagas' disease is well-established and reproduces important features of the human infection, providing an experimental basis for the study of host lineages and parasite strains. Thus, we evaluated acute and chronic infection by the G (TcI) and CL (TcVI) strains of T. cruzi, which have distinct tropisms and infectivity, in two inbred mice lineages (C57BL/6 and BALB/c) that display variable degrees of susceptibility to different T. cruzi strains. Analysis of the parasite loads in host tissues by qPCR showed that CL strain established an infection faster than the G strainat the same time, the response in BALB/c mice, although diverse in terms of cytokine secretion, was initiated earlier than that in C57BL/6 mice. At the parasitemia peak in the acute phase, we observed, either by confocal microscopy or by qPCR, that the infection was disseminated in all groups analyzed, with some differences concerning parasite tropismat this point, all animals responded to infection by increasing the serum concentrations of cytokines. However, BALB/c mice seemed to better regulate the immune response than C57BL/6 mice. Indeed, in the chronic phase, C57BL/6 mice still presented exacerbated cytokine and chemokine responses. In summary, our results indicate that in these experimental models, the deregulation of immune response that is typical of chronic Chagas' disease may be due to control loss over pro-and anti-inflammatory cytokines early in the acute phase of the disease, depending primarily on the host background rather than the parasite strain.Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo, BrazilWeb of ScienceFAPESPCAPESCNPqFAPESP: 2011/51475-3FAPESP: 2014/21338-2CNPq: 302068/2016-3Frontiers Media Sa2020-07-20T16:31:14Z2020-07-20T16:31:14Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.3389/fmicb.2018.00553Frontiers In Microbiology. Lausanne, v. 9, 2018.10.3389/fmicb.2018.00553WOS000428259600001.pdf1664-302Xhttps://repositorio.unifesp.br/handle/11600/55802WOS:000428259600001ark:/48912/001300000hqcvengFrontiers In MicrobiologyLausanneinfo:eu-repo/semantics/openAccessFerreira, Bianca L. [UNIFESP]Ferreira, Eden Ramalho [UNIFESP]Brito, Marlon Vilela de [UNIFESP]Salu, Bruno Ramos [UNIFESP]Oliva, Maria Luiza Vilela [UNIFESP]Mortara, Renato Arruda [UNIFESP]Orikaza, Cristina Mary [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-02T05:44:27Zoai:repositorio.unifesp.br/:11600/55802Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:19:42.277167Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| title |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| spellingShingle |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity Ferreira, Bianca L. [UNIFESP] Chagas' disease Trypanosoma cruzi immune response cytokines mice |
| title_short |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| title_full |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| title_fullStr |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| title_full_unstemmed |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| title_sort |
BALB/c and C57BL/6 Mice Cytokine Responses to Trypanosoma cruzi Infection Are Independent of Parasite Strain Infectivity |
| author |
Ferreira, Bianca L. [UNIFESP] |
| author_facet |
Ferreira, Bianca L. [UNIFESP] Ferreira, Eden Ramalho [UNIFESP] Brito, Marlon Vilela de [UNIFESP] Salu, Bruno Ramos [UNIFESP] Oliva, Maria Luiza Vilela [UNIFESP] Mortara, Renato Arruda [UNIFESP] Orikaza, Cristina Mary [UNIFESP] |
| author_role |
author |
| author2 |
Ferreira, Eden Ramalho [UNIFESP] Brito, Marlon Vilela de [UNIFESP] Salu, Bruno Ramos [UNIFESP] Oliva, Maria Luiza Vilela [UNIFESP] Mortara, Renato Arruda [UNIFESP] Orikaza, Cristina Mary [UNIFESP] |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Bianca L. [UNIFESP] Ferreira, Eden Ramalho [UNIFESP] Brito, Marlon Vilela de [UNIFESP] Salu, Bruno Ramos [UNIFESP] Oliva, Maria Luiza Vilela [UNIFESP] Mortara, Renato Arruda [UNIFESP] Orikaza, Cristina Mary [UNIFESP] |
| dc.subject.por.fl_str_mv |
Chagas' disease Trypanosoma cruzi immune response cytokines mice |
| topic |
Chagas' disease Trypanosoma cruzi immune response cytokines mice |
| description |
Trypanosoma cruzi is the etiologic agent of Chagas' disease, which affects 6-7 million people worldwide. Different strains of T. cruzi present specific genotypic and phenotypic characteristics that affect the host-pathogen interactions, and thus, the parasite has been classified into six groups (TcI to TcVI). T. cruzi infection presents two clinical phases, acute and chronic, both with distinct characteristics and important participation by the immune system. However, the specific contributions of parasite and host factors in the disease phases are not yet fully understood. The murine model for Chagas' disease is well-established and reproduces important features of the human infection, providing an experimental basis for the study of host lineages and parasite strains. Thus, we evaluated acute and chronic infection by the G (TcI) and CL (TcVI) strains of T. cruzi, which have distinct tropisms and infectivity, in two inbred mice lineages (C57BL/6 and BALB/c) that display variable degrees of susceptibility to different T. cruzi strains. Analysis of the parasite loads in host tissues by qPCR showed that CL strain established an infection faster than the G strain |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2020-07-20T16:31:14Z 2020-07-20T16:31:14Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2018.00553 Frontiers In Microbiology. Lausanne, v. 9, 2018. 10.3389/fmicb.2018.00553 WOS000428259600001.pdf 1664-302X https://repositorio.unifesp.br/handle/11600/55802 WOS:000428259600001 |
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ark:/48912/001300000hqcv |
| url |
http://dx.doi.org/10.3389/fmicb.2018.00553 https://repositorio.unifesp.br/handle/11600/55802 |
| identifier_str_mv |
Frontiers In Microbiology. Lausanne, v. 9, 2018. 10.3389/fmicb.2018.00553 WOS000428259600001.pdf 1664-302X WOS:000428259600001 ark:/48912/001300000hqcv |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Frontiers In Microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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- application/pdf |
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Lausanne |
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Frontiers Media Sa |
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Frontiers Media Sa |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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