Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/55113 http://dx.doi.org/10.1371/journal.pone.0171385 |
Resumo: | Diarrhea is the second leading cause of death of children up to five years old in the developing countries. Among the etiological diarrheal agents are atypical enteropathogenic Escherichia coli (aEPEC), one of the diarrheagenic E. coli pathotypes that affects children and adults, even in developed countries. Currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aEPEC are actually E. albertii, a recently recognized human enteropathogen. Studies on particular strains are necessary to explore their virulence potential in order to further understand the underlying mechanisms of E. albertii infections. Here we demonstrated for the first time that infection of fragments of rat intestinal mucosa is a useful tool to study the initial steps of E. albertii colonization. We also observed that an E. albertii strain can translocate from the intestinal lumen to Mesenteric Lymph Nodes and liver in a rat model. Based on our finding of bacterial translocation, we investigated how E. albertii might cross the intestinal epithelium by performing infections of M-like cells in vitro to identify the potential in vivo translocation route. Altogether, our approaches allowed us to draft a general E. albertii infection route from the colonization till the bacterial spreading in vivo. |
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Yamamoto, Denise [UNIFESP]Hernandes, Rodrigo T. [UNIFESP]Liberatore, Ana Maria A. [UNIFESP]Abe, Cecilia M.de Souza, Rodrigo B. [UNIFESP]Romao, Fabiano T. [UNIFESP]Sperandio, VanessaKoh, Ivan H. [UNIFESP]Gomes, Tania A. T. [UNIFESP]2020-07-17T14:02:58Z2020-07-17T14:02:58Z2017Plos One. San Francisco, v. 12, n. 2, p. -, 2017.1932-6203https://repositorio.unifesp.br/handle/11600/55113http://dx.doi.org/10.1371/journal.pone.0171385WOS000393712500039.pdf10.1371/journal.pone.0171385WOS:000393712500039Diarrhea is the second leading cause of death of children up to five years old in the developing countries. Among the etiological diarrheal agents are atypical enteropathogenic Escherichia coli (aEPEC), one of the diarrheagenic E. coli pathotypes that affects children and adults, even in developed countries. Currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aEPEC are actually E. albertii, a recently recognized human enteropathogen. Studies on particular strains are necessary to explore their virulence potential in order to further understand the underlying mechanisms of E. albertii infections. Here we demonstrated for the first time that infection of fragments of rat intestinal mucosa is a useful tool to study the initial steps of E. albertii colonization. We also observed that an E. albertii strain can translocate from the intestinal lumen to Mesenteric Lymph Nodes and liver in a rat model. Based on our finding of bacterial translocation, we investigated how E. albertii might cross the intestinal epithelium by performing infections of M-like cells in vitro to identify the potential in vivo translocation route. Altogether, our approaches allowed us to draft a general E. albertii infection route from the colonization till the bacterial spreading in vivo.FUNDACAO DE AM PARO A PESQUISA DO ESTADO DE SAO PAULO (FAPESP)COORDENACAO DE APERFEICOAMENTO DE PESSOAL DE NIVEL SUPERIOR (CAPES)Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilUniv Estadual Paulista, UNESP, Dept Microbiol & Imunol, Inst Biociencia, Botucatu, SP, BrazilUniv Fed Sao Paulo, Dept Cirurgia, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilInst Butantan, Lab Biol Celular, Sao Paulo, BrazilUniv Texas Southwestern Med Ctr Dallas, Dept Microbiol & Biochem, Dallas, TX 75390 USAUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Cirurgia, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilFAPESP: 2011/12664-5CAPES: AUX-PE-PNPD 2350/2011Web of Science-engPublic Library SciencePlos OneEscherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSan Francisco122info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000393712500039.pdfapplication/pdf1779176${dspace.ui.url}/bitstream/11600/55113/1/WOS000393712500039.pdf7cf48613801c413f1dc21b77ab440b2eMD51open accessTEXTWOS000393712500039.pdf.txtWOS000393712500039.pdf.txtExtracted texttext/plain56230${dspace.ui.url}/bitstream/11600/55113/2/WOS000393712500039.pdf.txt94a40e216f4cf9774f8334672c67e811MD52open accessTHUMBNAILWOS000393712500039.pdf.jpgWOS000393712500039.pdf.jpgIM Thumbnailimage/jpeg7564${dspace.ui.url}/bitstream/11600/55113/4/WOS000393712500039.pdf.jpg5414e9c083d8f2dad86a07b4dda2506cMD54open access11600/551132022-08-01 04:49:47.796open accessoai:repositorio.unifesp.br:11600/55113Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T07:49:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
title |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
spellingShingle |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites Yamamoto, Denise [UNIFESP] |
title_short |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
title_full |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
title_fullStr |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
title_full_unstemmed |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
title_sort |
Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites |
author |
Yamamoto, Denise [UNIFESP] |
author_facet |
Yamamoto, Denise [UNIFESP] Hernandes, Rodrigo T. [UNIFESP] Liberatore, Ana Maria A. [UNIFESP] Abe, Cecilia M. de Souza, Rodrigo B. [UNIFESP] Romao, Fabiano T. [UNIFESP] Sperandio, Vanessa Koh, Ivan H. [UNIFESP] Gomes, Tania A. T. [UNIFESP] |
author_role |
author |
author2 |
Hernandes, Rodrigo T. [UNIFESP] Liberatore, Ana Maria A. [UNIFESP] Abe, Cecilia M. de Souza, Rodrigo B. [UNIFESP] Romao, Fabiano T. [UNIFESP] Sperandio, Vanessa Koh, Ivan H. [UNIFESP] Gomes, Tania A. T. [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Yamamoto, Denise [UNIFESP] Hernandes, Rodrigo T. [UNIFESP] Liberatore, Ana Maria A. [UNIFESP] Abe, Cecilia M. de Souza, Rodrigo B. [UNIFESP] Romao, Fabiano T. [UNIFESP] Sperandio, Vanessa Koh, Ivan H. [UNIFESP] Gomes, Tania A. T. [UNIFESP] |
description |
Diarrhea is the second leading cause of death of children up to five years old in the developing countries. Among the etiological diarrheal agents are atypical enteropathogenic Escherichia coli (aEPEC), one of the diarrheagenic E. coli pathotypes that affects children and adults, even in developed countries. Currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aEPEC are actually E. albertii, a recently recognized human enteropathogen. Studies on particular strains are necessary to explore their virulence potential in order to further understand the underlying mechanisms of E. albertii infections. Here we demonstrated for the first time that infection of fragments of rat intestinal mucosa is a useful tool to study the initial steps of E. albertii colonization. We also observed that an E. albertii strain can translocate from the intestinal lumen to Mesenteric Lymph Nodes and liver in a rat model. Based on our finding of bacterial translocation, we investigated how E. albertii might cross the intestinal epithelium by performing infections of M-like cells in vitro to identify the potential in vivo translocation route. Altogether, our approaches allowed us to draft a general E. albertii infection route from the colonization till the bacterial spreading in vivo. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-17T14:02:58Z |
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2020-07-17T14:02:58Z |
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article |
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dc.identifier.citation.fl_str_mv |
Plos One. San Francisco, v. 12, n. 2, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/55113 http://dx.doi.org/10.1371/journal.pone.0171385 |
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1932-6203 |
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WOS000393712500039.pdf |
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10.1371/journal.pone.0171385 |
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Plos One. San Francisco, v. 12, n. 2, p. -, 2017. 1932-6203 WOS000393712500039.pdf 10.1371/journal.pone.0171385 WOS:000393712500039 |
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https://repositorio.unifesp.br/handle/11600/55113 http://dx.doi.org/10.1371/journal.pone.0171385 |
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