Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/1809 http://dx.doi.org/10.1590/S0100-879X2003000800010 |
Resumo: | Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway. |
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Nunes, Viviane A. [UNIFESP]Gozzo, Andrezza Justino [UNIFESP]Juliano, Maria Aparecida [UNIFESP]Cerqueira César, M.Sampaio, Misako Uemura [UNIFESP]Sampaio, Claudio a.m. [UNIFESP]Araujo, Mariana da Silva [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2015-06-14T13:30:06Z2015-06-14T13:30:06Z2003-08-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003.0100-879Xhttp://repositorio.unifesp.br/handle/11600/1809http://dx.doi.org/10.1590/S0100-879X2003000800010S0100-879X2003000800010.pdfS0100-879X200300080001010.1590/S0100-879X2003000800010WOS:000185012900011Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BiofísicaUniversidade de São Paulo Faculdade de Zootecnia e Engenharia de Alimentos Departamento de CiênciasUNIFESP, EPM, Depto. de BioquímicaUNIFESP, EPM, Depto. de BiofísicaSciELO1047-1053engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchOxidative stressCaspase activationApoptosisDietary selenium deficiencyDietary vitamin E deficiencyInternucleosomal DNA fragmentationCaspase substrateAntioxidant dietary deficiency induces caspase activation in chick skeletal muscle cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2003000800010.pdfapplication/pdf1010675${dspace.ui.url}/bitstream/11600/1809/1/S0100-879X2003000800010.pdf434f0802b490bb6c9d2f4324faf9283dMD51open accessTEXTS0100-879X2003000800010.pdf.txtS0100-879X2003000800010.pdf.txtExtracted texttext/plain24763${dspace.ui.url}/bitstream/11600/1809/2/S0100-879X2003000800010.pdf.txt9d3eddb44d7458f8615beed33d15c8baMD52open access11600/18092021-09-29 11:23:43.239open accessoai:repositorio.unifesp.br:11600/1809Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-29T14:23:43Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
spellingShingle |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells Nunes, Viviane A. [UNIFESP] Oxidative stress Caspase activation Apoptosis Dietary selenium deficiency Dietary vitamin E deficiency Internucleosomal DNA fragmentation Caspase substrate |
title_short |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_full |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_fullStr |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_full_unstemmed |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
title_sort |
Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells |
author |
Nunes, Viviane A. [UNIFESP] |
author_facet |
Nunes, Viviane A. [UNIFESP] Gozzo, Andrezza Justino [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Cerqueira César, M. Sampaio, Misako Uemura [UNIFESP] Sampaio, Claudio a.m. [UNIFESP] Araujo, Mariana da Silva [UNIFESP] |
author_role |
author |
author2 |
Gozzo, Andrezza Justino [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Cerqueira César, M. Sampaio, Misako Uemura [UNIFESP] Sampaio, Claudio a.m. [UNIFESP] Araujo, Mariana da Silva [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nunes, Viviane A. [UNIFESP] Gozzo, Andrezza Justino [UNIFESP] Juliano, Maria Aparecida [UNIFESP] Cerqueira César, M. Sampaio, Misako Uemura [UNIFESP] Sampaio, Claudio a.m. [UNIFESP] Araujo, Mariana da Silva [UNIFESP] |
dc.subject.eng.fl_str_mv |
Oxidative stress Caspase activation Apoptosis Dietary selenium deficiency Dietary vitamin E deficiency Internucleosomal DNA fragmentation Caspase substrate |
topic |
Oxidative stress Caspase activation Apoptosis Dietary selenium deficiency Dietary vitamin E deficiency Internucleosomal DNA fragmentation Caspase substrate |
description |
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm. Proteolytic activation was not accompanied by typical internucleosomal DNA fragmentation detected by field inversion gel electrophoresis. Although the general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk) (0 to 80 muM) did not block caspase-like activity when preincubated for 30 min with muscle homogenates, the hydrolyzed substrates presented the same cleavage profile in HPLC (at the aspartic acid residue) when incubated with the purified recombinant enzyme caspase-3. These data indicate that oxidative stress causes caspase-like activation in muscle cells and suggest that cell death associated with exudative diathesis (dietary deficiency of selenium and vitamin E) can follow the apoptotic pathway. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003-08-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:30:06Z |
dc.date.available.fl_str_mv |
2015-06-14T13:30:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/1809 http://dx.doi.org/10.1590/S0100-879X2003000800010 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2003000800010.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2003000800010 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2003000800010 |
dc.identifier.wos.none.fl_str_mv |
WOS:000185012900011 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1047-1053, 2003. 0100-879X S0100-879X2003000800010.pdf S0100-879X2003000800010 10.1590/S0100-879X2003000800010 WOS:000185012900011 |
url |
http://repositorio.unifesp.br/handle/11600/1809 http://dx.doi.org/10.1590/S0100-879X2003000800010 |
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eng |
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Brazilian Journal of Medical and Biological Research |
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openAccess |
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1047-1053 |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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