Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity

Detalhes bibliográficos
Autor(a) principal: Leite, João Paulo V.
Data de Publicação: 2010
Outros Autores: Braga, Fernão C., Romussi, Giovanni, Persoli, Rita M. [UNIFESP], Tabach, Ricardo [UNIFESP], Carlini, Elisaldo Araujo [UNIFESP], Oliveira, Alaíde B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0103-50532010000200009
http://repositorio.unifesp.br/handle/11600/5510
Resumo: Maytenus ilicifolia Mart. ex Reissek is traditionally used in Brazil for treatment of gastric ulcers. Here we report the phytochemical investigation of an ethanol extract of M. ilicifolia leaves (EEMIL) aiming at the isolation of constituents which were used as chemical markers to monitor an activity-guided fractionation of a lyophilized aqueous extract of M. ilicifolia leaves (LAEMIL). From EEMIL, four flavonoids were isolated, namely the tri-flavonoid glycosides mauritianin (1), trifolin, (2) hyperin (4), and epi-catechin (5). Fractionation of LAEMIL led to 5 fractions which afforded the tetra-glycoside kaempferol derivative (3), and galactitol (6). LAEMIL and its fractions were evaluated in rats for their effects on gastric secretion volume and pH. HPLC (High Performance Liquid Chromatography) analysis revealed that only fractions containing the tri- and tetra-flavonoid glycosides 1 and 3 caused significant increase of gastric volume and pH, thus indicating that these glycosides play an important role on the gastroprotective effect of M.ilicifolia leaves.
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spelling Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activityMaytenus ilicifoliaflavonoid glycosidesgastroprotective activityliquid chromatographyMaytenus ilicifolia Mart. ex Reissek is traditionally used in Brazil for treatment of gastric ulcers. Here we report the phytochemical investigation of an ethanol extract of M. ilicifolia leaves (EEMIL) aiming at the isolation of constituents which were used as chemical markers to monitor an activity-guided fractionation of a lyophilized aqueous extract of M. ilicifolia leaves (LAEMIL). From EEMIL, four flavonoids were isolated, namely the tri-flavonoid glycosides mauritianin (1), trifolin, (2) hyperin (4), and epi-catechin (5). Fractionation of LAEMIL led to 5 fractions which afforded the tetra-glycoside kaempferol derivative (3), and galactitol (6). LAEMIL and its fractions were evaluated in rats for their effects on gastric secretion volume and pH. HPLC (High Performance Liquid Chromatography) analysis revealed that only fractions containing the tri- and tetra-flavonoid glycosides 1 and 3 caused significant increase of gastric volume and pH, thus indicating that these glycosides play an important role on the gastroprotective effect of M.ilicifolia leaves.Maytenus ilicifolia Mart. ex Reissek é tradicionalmente usada no Brasil para o tratamento de úlcera gástrica. O presente trabalho relata a investigação fitoquímica de um extrato etanólico de folhas de M. ilicifolia (EEMIL) visando o isolamento de constituintes que foram usados como marcadores químicos para monitorar o fracionamento de um extrato aquoso liofilizado de folhas de M. ilicifolia (LAEMIL). De EEMIL, quatro flavonóides foram isolados, compreendendo o triglicosídeo flavônico mauritianina (1), trifolina (2), hyperina (4), e epi-catequina (5). O fracionamento de LAEMIL levou a 5 frações, fornecendo um derivado tetraglicosilado de canferol (3), além do galactitol (6). LAEMIL e suas frações foram avaliadas quanto aos efeitos sobre o volume e pH da secreção gástrica em ratos. Análise por CLAE (Cromatografia Líquida de Alta Eficiência) revelou que somente frações contendo o tri- e tetra-glicosídeos flavônicos 1 e 3 causaram aumento significativo de volume gástrico e pH, indicando que esses glicosídeos têm importante papel sob o efeito gastroprotetor de folhas de M. ilicifolia.Universidade Federal de Viçosa Departamento de Bioquímica e Biologia MolecularUniversidade Federal de Minas Gerais Faculdade de Farmácia Departamento de Produtos FarmacêuticosUniversità degli Studi di Genova Dipartamento di Chimica e Tecnologie FarmaceuticheUniversidade Federal de São Paulo (UNIFESP) Departamento de PsicobiologiaUNIFESP, Depto. de PsicobiologiaSciELOConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira de QuímicaUniversidade Federal de Viçosa Departamento de Bioquímica e Biologia MolecularUniversidade Federal de Minas Gerais Faculdade de Farmácia Departamento de Produtos FarmacêuticosUniversità degli Studi di Genova Dipartamento di Chimica e Tecnologie FarmaceuticheUniversidade Federal de São Paulo (UNIFESP)Leite, João Paulo V.Braga, Fernão C.Romussi, GiovanniPersoli, Rita M. [UNIFESP]Tabach, Ricardo [UNIFESP]Carlini, Elisaldo Araujo [UNIFESP]Oliveira, Alaíde B.2015-06-14T13:41:26Z2015-06-14T13:41:26Z2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion248-254application/pdfhttp://dx.doi.org/10.1590/S0103-50532010000200009Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 21, n. 2, p. 248-254, 2010.10.1590/S0103-50532010000200009S0103-50532010000200009.pdf0103-5053S0103-50532010000200009http://repositorio.unifesp.br/handle/11600/5510WOS:000276085500009engJournal of the Brazilian Chemical Societyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T02:48:37Zoai:repositorio.unifesp.br/:11600/5510Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T02:48:37Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
title Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
spellingShingle Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
Leite, João Paulo V.
Maytenus ilicifolia
flavonoid glycosides
gastroprotective activity
liquid chromatography
title_short Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
title_full Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
title_fullStr Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
title_full_unstemmed Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
title_sort Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity
author Leite, João Paulo V.
author_facet Leite, João Paulo V.
Braga, Fernão C.
Romussi, Giovanni
Persoli, Rita M. [UNIFESP]
Tabach, Ricardo [UNIFESP]
Carlini, Elisaldo Araujo [UNIFESP]
Oliveira, Alaíde B.
author_role author
author2 Braga, Fernão C.
Romussi, Giovanni
Persoli, Rita M. [UNIFESP]
Tabach, Ricardo [UNIFESP]
Carlini, Elisaldo Araujo [UNIFESP]
Oliveira, Alaíde B.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa Departamento de Bioquímica e Biologia Molecular
Universidade Federal de Minas Gerais Faculdade de Farmácia Departamento de Produtos Farmacêuticos
Università degli Studi di Genova Dipartamento di Chimica e Tecnologie Farmaceutiche
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Leite, João Paulo V.
Braga, Fernão C.
Romussi, Giovanni
Persoli, Rita M. [UNIFESP]
Tabach, Ricardo [UNIFESP]
Carlini, Elisaldo Araujo [UNIFESP]
Oliveira, Alaíde B.
dc.subject.por.fl_str_mv Maytenus ilicifolia
flavonoid glycosides
gastroprotective activity
liquid chromatography
topic Maytenus ilicifolia
flavonoid glycosides
gastroprotective activity
liquid chromatography
description Maytenus ilicifolia Mart. ex Reissek is traditionally used in Brazil for treatment of gastric ulcers. Here we report the phytochemical investigation of an ethanol extract of M. ilicifolia leaves (EEMIL) aiming at the isolation of constituents which were used as chemical markers to monitor an activity-guided fractionation of a lyophilized aqueous extract of M. ilicifolia leaves (LAEMIL). From EEMIL, four flavonoids were isolated, namely the tri-flavonoid glycosides mauritianin (1), trifolin, (2) hyperin (4), and epi-catechin (5). Fractionation of LAEMIL led to 5 fractions which afforded the tetra-glycoside kaempferol derivative (3), and galactitol (6). LAEMIL and its fractions were evaluated in rats for their effects on gastric secretion volume and pH. HPLC (High Performance Liquid Chromatography) analysis revealed that only fractions containing the tri- and tetra-flavonoid glycosides 1 and 3 caused significant increase of gastric volume and pH, thus indicating that these glycosides play an important role on the gastroprotective effect of M.ilicifolia leaves.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
2015-06-14T13:41:26Z
2015-06-14T13:41:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0103-50532010000200009
Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 21, n. 2, p. 248-254, 2010.
10.1590/S0103-50532010000200009
S0103-50532010000200009.pdf
0103-5053
S0103-50532010000200009
http://repositorio.unifesp.br/handle/11600/5510
WOS:000276085500009
url http://dx.doi.org/10.1590/S0103-50532010000200009
http://repositorio.unifesp.br/handle/11600/5510
identifier_str_mv Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 21, n. 2, p. 248-254, 2010.
10.1590/S0103-50532010000200009
S0103-50532010000200009.pdf
0103-5053
S0103-50532010000200009
WOS:000276085500009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the Brazilian Chemical Society
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 248-254
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268323568812032

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