A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/25729 http://dx.doi.org/10.1093/glycob/7.4.463 |
Resumo: | A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose. |
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Suzuki, Erika [UNIFESP]Toledo, Marcos Sergio de [UNIFESP]Takahashi, Helio Kiyoshi [UNIFESP]Straus, Anita Hilda [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:30:21Z2016-01-24T12:30:21Z1997-06-01Glycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997.0959-6658http://repositorio.unifesp.br/handle/11600/25729http://dx.doi.org/10.1093/glycob/7.4.46310.1093/glycob/7.4.463WOS:A1997XB69300003A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose.Universidade Federal de São Paulo,DEPT BIOCHEM,EPM,BR-04023900 São Paulo,BRAZILUniversidade Federal de São Paulo,DEPT BIOCHEM,EPM,BR-04023900 São Paulo,BRAZILWeb of Science463-468engOxford Univ PressGlycobiologyhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccessgalactofuranoseglycolipid antigenmonoclonal antibodyParacoccidioides brasiliensisLeishmania majorA monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/257292022-11-03 10:40:51.693metadata only accessoai:repositorio.unifesp.br:11600/25729Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-03T13:40:51Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
title |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
spellingShingle |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi Suzuki, Erika [UNIFESP] galactofuranose glycolipid antigen monoclonal antibody Paracoccidioides brasiliensis Leishmania major |
title_short |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
title_full |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
title_fullStr |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
title_full_unstemmed |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
title_sort |
A monoclonal antibody directed to terminal residue of beta-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis; Cross-reactivity with Leishmania major and Trypanosoma cruzi |
author |
Suzuki, Erika [UNIFESP] |
author_facet |
Suzuki, Erika [UNIFESP] Toledo, Marcos Sergio de [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] Straus, Anita Hilda [UNIFESP] |
author_role |
author |
author2 |
Toledo, Marcos Sergio de [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] Straus, Anita Hilda [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Suzuki, Erika [UNIFESP] Toledo, Marcos Sergio de [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] Straus, Anita Hilda [UNIFESP] |
dc.subject.eng.fl_str_mv |
galactofuranose glycolipid antigen monoclonal antibody Paracoccidioides brasiliensis Leishmania major |
topic |
galactofuranose glycolipid antigen monoclonal antibody Paracoccidioides brasiliensis Leishmania major |
description |
A mouse monoclonal antibody, MEST-1, was produced against Band 1 glycolipid antigen of Paracoccidioides brasiliensis, the glycan structure of Band 1 antigen was recently elucidated and the monosaccharides sequence was defined as: Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins. the reactivity of MEST-1 MAb was determined by solid-phase radioimmunoassay and high performance thin layer chromatography immunostaining. Selective oxidation of galactofuranose residues and inhibition assays with different methyl-glycosides, revealed that MAb MEST-1 is directed against the terminal residue of beta-D-gaiactofuranose of Band 1, a phosphoglyceroglycolipid antigen of P.brasiliensis. By indirect immunofluorescence, it was observed that the epitope recognized by MEST-1 is accessible to the antibody in yeast forms of this fungus. Reactivity of MEST-1 with parasites known to express galactofuranose containing glycoconjugates was also analyzed by indirect immunofluorescence. A positive fluorescence was observed with promastigotes of Leishmania major and epimastigotes of Trypanosoma cruzi, GIPL-1 was identified as the antigen recognized by MEST-1 in Leishmania major, indicating that the MAb MEST-1 recognizes terminal galactofuranose residue in either beta 1-->6 or beta 1-->3 linkage to the mannose. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997-06-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:30:21Z |
dc.date.available.fl_str_mv |
2016-01-24T12:30:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Glycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/25729 http://dx.doi.org/10.1093/glycob/7.4.463 |
dc.identifier.issn.none.fl_str_mv |
0959-6658 |
dc.identifier.doi.none.fl_str_mv |
10.1093/glycob/7.4.463 |
dc.identifier.wos.none.fl_str_mv |
WOS:A1997XB69300003 |
identifier_str_mv |
Glycobiology. Oxford: Oxford Univ Press, v. 7, n. 4, p. 463-468, 1997. 0959-6658 10.1093/glycob/7.4.463 WOS:A1997XB69300003 |
url |
http://repositorio.unifesp.br/handle/11600/25729 http://dx.doi.org/10.1093/glycob/7.4.463 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Glycobiology |
dc.rights.driver.fl_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
463-468 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764179609747456 |