Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.21577/0103-5053.20160301 http://repositorio.unifesp.br/handle/11600/51364 |
Resumo: | Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions. |
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Repositório Institucional da UNIFESP |
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Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detectionpeptide hydrogelsdiphenylalanineimmunosensorELISALeishmaniasisHydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions.Univ Fed, ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, BrazilUniv Fed São Paulo, Dept Biofis, BR-04023062 São Paulo, SP, BrazilUniv Fed São Paulo, Dept Biofis, BR-04023062 São Paulo, SP, BrazilWeb of ScienceFAPESPCNPqFAPESP: 2015/24018-1FAPESP: 08/57805-2FAPESP: 2012/01933-8FAPESP: 2013/12674-6CNPq: 302923/2015-2CNPq: 400239/2014-0CNPq: 573672/2008-3Soc Brasileira Quimica2019-08-19T11:49:38Z2019-08-19T11:49:38Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1619-1629application/pdfhttp://dx.doi.org/10.21577/0103-5053.20160301Journal Of The Brazilian Chemical Society. Sao Paulo, v. 28, n. 9, p. 1619-1629, 2017.10.21577/0103-5053.20160301WOS000406806200004.pdf0103-5053http://repositorio.unifesp.br/handle/11600/51364WOS:000406806200004enginfo:eu-repo/semantics/openAccessSouza, Sivoney F.Kogikoski, Sergio, Jr.Silva, Emerson R. [UNIFESP]Alves, Wendel A.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T01:40:07Zoai:repositorio.unifesp.br/:11600/51364Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T01:40:07Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
title |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
spellingShingle |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection Souza, Sivoney F. peptide hydrogels diphenylalanine immunosensor ELISA Leishmaniasis |
title_short |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
title_full |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
title_fullStr |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
title_full_unstemmed |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
title_sort |
Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection |
author |
Souza, Sivoney F. |
author_facet |
Souza, Sivoney F. Kogikoski, Sergio, Jr. Silva, Emerson R. [UNIFESP] Alves, Wendel A. |
author_role |
author |
author2 |
Kogikoski, Sergio, Jr. Silva, Emerson R. [UNIFESP] Alves, Wendel A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Souza, Sivoney F. Kogikoski, Sergio, Jr. Silva, Emerson R. [UNIFESP] Alves, Wendel A. |
dc.subject.por.fl_str_mv |
peptide hydrogels diphenylalanine immunosensor ELISA Leishmaniasis |
topic |
peptide hydrogels diphenylalanine immunosensor ELISA Leishmaniasis |
description |
Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2019-08-19T11:49:38Z 2019-08-19T11:49:38Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.21577/0103-5053.20160301 Journal Of The Brazilian Chemical Society. Sao Paulo, v. 28, n. 9, p. 1619-1629, 2017. 10.21577/0103-5053.20160301 WOS000406806200004.pdf 0103-5053 http://repositorio.unifesp.br/handle/11600/51364 WOS:000406806200004 |
url |
http://dx.doi.org/10.21577/0103-5053.20160301 http://repositorio.unifesp.br/handle/11600/51364 |
identifier_str_mv |
Journal Of The Brazilian Chemical Society. Sao Paulo, v. 28, n. 9, p. 1619-1629, 2017. 10.21577/0103-5053.20160301 WOS000406806200004.pdf 0103-5053 WOS:000406806200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1619-1629 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Quimica |
publisher.none.fl_str_mv |
Soc Brasileira Quimica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268451739402240 |