Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection

Detalhes bibliográficos
Autor(a) principal: Souza, Sivoney F.
Data de Publicação: 2017
Outros Autores: Kogikoski, Sergio, Jr., Silva, Emerson R. [UNIFESP], Alves, Wendel A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.21577/0103-5053.20160301
http://repositorio.unifesp.br/handle/11600/51364
Resumo: Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions.
id UFSP_4615f4746bb1815afa011dc89d16933b
oai_identifier_str oai:repositorio.unifesp.br/:11600/51364
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detectionpeptide hydrogelsdiphenylalanineimmunosensorELISALeishmaniasisHydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions.Univ Fed, ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP, BrazilUniv Fed São Paulo, Dept Biofis, BR-04023062 São Paulo, SP, BrazilUniv Fed São Paulo, Dept Biofis, BR-04023062 São Paulo, SP, BrazilWeb of ScienceFAPESPCNPqFAPESP: 2015/24018-1FAPESP: 08/57805-2FAPESP: 2012/01933-8FAPESP: 2013/12674-6CNPq: 302923/2015-2CNPq: 400239/2014-0CNPq: 573672/2008-3Soc Brasileira Quimica2019-08-19T11:49:38Z2019-08-19T11:49:38Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1619-1629application/pdfhttp://dx.doi.org/10.21577/0103-5053.20160301Journal Of The Brazilian Chemical Society. Sao Paulo, v. 28, n. 9, p. 1619-1629, 2017.10.21577/0103-5053.20160301WOS000406806200004.pdf0103-5053http://repositorio.unifesp.br/handle/11600/51364WOS:000406806200004enginfo:eu-repo/semantics/openAccessSouza, Sivoney F.Kogikoski, Sergio, Jr.Silva, Emerson R. [UNIFESP]Alves, Wendel A.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T01:40:07Zoai:repositorio.unifesp.br/:11600/51364Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T01:40:07Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
title Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
spellingShingle Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
Souza, Sivoney F.
peptide hydrogels
diphenylalanine
immunosensor
ELISA
Leishmaniasis
title_short Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
title_full Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
title_fullStr Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
title_full_unstemmed Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
title_sort Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection
author Souza, Sivoney F.
author_facet Souza, Sivoney F.
Kogikoski, Sergio, Jr.
Silva, Emerson R. [UNIFESP]
Alves, Wendel A.
author_role author
author2 Kogikoski, Sergio, Jr.
Silva, Emerson R. [UNIFESP]
Alves, Wendel A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Souza, Sivoney F.
Kogikoski, Sergio, Jr.
Silva, Emerson R. [UNIFESP]
Alves, Wendel A.
dc.subject.por.fl_str_mv peptide hydrogels
diphenylalanine
immunosensor
ELISA
Leishmaniasis
topic peptide hydrogels
diphenylalanine
immunosensor
ELISA
Leishmaniasis
description Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L, L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019-08-19T11:49:38Z
2019-08-19T11:49:38Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.21577/0103-5053.20160301
Journal Of The Brazilian Chemical Society. Sao Paulo, v. 28, n. 9, p. 1619-1629, 2017.
10.21577/0103-5053.20160301
WOS000406806200004.pdf
0103-5053
http://repositorio.unifesp.br/handle/11600/51364
WOS:000406806200004
url http://dx.doi.org/10.21577/0103-5053.20160301
http://repositorio.unifesp.br/handle/11600/51364
identifier_str_mv Journal Of The Brazilian Chemical Society. Sao Paulo, v. 28, n. 9, p. 1619-1629, 2017.
10.21577/0103-5053.20160301
WOS000406806200004.pdf
0103-5053
WOS:000406806200004
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1619-1629
application/pdf
dc.publisher.none.fl_str_mv Soc Brasileira Quimica
publisher.none.fl_str_mv Soc Brasileira Quimica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268451739402240