Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C

Detalhes bibliográficos
Autor(a) principal: Lima, Lais R. [UNESP]
Data de Publicação: 2016
Outros Autores: Moraes, Marli L., Nigoghossian, Karina [UNESP], Peres, Maristela F.S. [UNESP], Ribeiro, Sidney J.L. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jlumin.2015.08.039
http://hdl.handle.net/11449/177721
Resumo: Nanostructured films prepared by Layer-by-Layer technique and containing silk fibroin, antigenic peptide NS5A-1 derived from hepatitis C virus (HCV) NS5A protein and YVO4:Eu3+ luminescent nanoparticles, were utilized in sensing of hepatitis C. Detection system exploits the biorecognition between the antibody anti-HCV and the antigenic peptide NS5A-1 through changes in luminescence properties. Films deposition was monitored by UV-vis Absorption and Fluorescence Spectroscopy measurements at each bilayer deposited. The Eu3+ luminescence properties were evaluated in the presence of anti-HCV for optical detection of specific antibody and anti-HIV used as negative control. Significant changes in luminescence were observed in the presence of anti-HCV concentrations. A new immunosensor platform is proposed for optical detection of hepatitis C.
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spelling Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis CAntigenic peptideEuropiumImmunosensorLuminescent nanoparticlesSilk fibroinNanostructured films prepared by Layer-by-Layer technique and containing silk fibroin, antigenic peptide NS5A-1 derived from hepatitis C virus (HCV) NS5A protein and YVO4:Eu3+ luminescent nanoparticles, were utilized in sensing of hepatitis C. Detection system exploits the biorecognition between the antibody anti-HCV and the antigenic peptide NS5A-1 through changes in luminescence properties. Films deposition was monitored by UV-vis Absorption and Fluorescence Spectroscopy measurements at each bilayer deposited. The Eu3+ luminescence properties were evaluated in the presence of anti-HCV for optical detection of specific antibody and anti-HIV used as negative control. Significant changes in luminescence were observed in the presence of anti-HCV concentrations. A new immunosensor platform is proposed for optical detection of hepatitis C.Institute of Chemistry São Paulo State University UNESP, CP355Institute of Science and Technology Federal University of São Paulo UNIFESPInstitute of Chemistry São Paulo State University UNESP, CP355Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Lima, Lais R. [UNESP]Moraes, Marli L.Nigoghossian, Karina [UNESP]Peres, Maristela F.S. [UNESP]Ribeiro, Sidney J.L. [UNESP]2018-12-11T17:26:47Z2018-12-11T17:26:47Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article375-379application/pdfhttp://dx.doi.org/10.1016/j.jlumin.2015.08.039Journal of Luminescence, v. 170, p. 375-379.0022-2313http://hdl.handle.net/11449/17772110.1016/j.jlumin.2015.08.0392-s2.0-849543755782-s2.0-84954375578.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Luminescence0,694info:eu-repo/semantics/openAccess2023-10-02T06:06:37Zoai:repositorio.unesp.br:11449/177721Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:47:31.633082Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
title Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
spellingShingle Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
Lima, Lais R. [UNESP]
Antigenic peptide
Europium
Immunosensor
Luminescent nanoparticles
Silk fibroin
title_short Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
title_full Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
title_fullStr Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
title_full_unstemmed Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
title_sort Silk fibroin-antigenic peptides-YVO4:Eu3+ nanostructured thin films as sensors for hepatitis C
author Lima, Lais R. [UNESP]
author_facet Lima, Lais R. [UNESP]
Moraes, Marli L.
Nigoghossian, Karina [UNESP]
Peres, Maristela F.S. [UNESP]
Ribeiro, Sidney J.L. [UNESP]
author_role author
author2 Moraes, Marli L.
Nigoghossian, Karina [UNESP]
Peres, Maristela F.S. [UNESP]
Ribeiro, Sidney J.L. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Lima, Lais R. [UNESP]
Moraes, Marli L.
Nigoghossian, Karina [UNESP]
Peres, Maristela F.S. [UNESP]
Ribeiro, Sidney J.L. [UNESP]
dc.subject.por.fl_str_mv Antigenic peptide
Europium
Immunosensor
Luminescent nanoparticles
Silk fibroin
topic Antigenic peptide
Europium
Immunosensor
Luminescent nanoparticles
Silk fibroin
description Nanostructured films prepared by Layer-by-Layer technique and containing silk fibroin, antigenic peptide NS5A-1 derived from hepatitis C virus (HCV) NS5A protein and YVO4:Eu3+ luminescent nanoparticles, were utilized in sensing of hepatitis C. Detection system exploits the biorecognition between the antibody anti-HCV and the antigenic peptide NS5A-1 through changes in luminescence properties. Films deposition was monitored by UV-vis Absorption and Fluorescence Spectroscopy measurements at each bilayer deposited. The Eu3+ luminescence properties were evaluated in the presence of anti-HCV for optical detection of specific antibody and anti-HIV used as negative control. Significant changes in luminescence were observed in the presence of anti-HCV concentrations. A new immunosensor platform is proposed for optical detection of hepatitis C.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
2018-12-11T17:26:47Z
2018-12-11T17:26:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jlumin.2015.08.039
Journal of Luminescence, v. 170, p. 375-379.
0022-2313
http://hdl.handle.net/11449/177721
10.1016/j.jlumin.2015.08.039
2-s2.0-84954375578
2-s2.0-84954375578.pdf
url http://dx.doi.org/10.1016/j.jlumin.2015.08.039
http://hdl.handle.net/11449/177721
identifier_str_mv Journal of Luminescence, v. 170, p. 375-379.
0022-2313
10.1016/j.jlumin.2015.08.039
2-s2.0-84954375578
2-s2.0-84954375578.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Luminescence
0,694
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 375-379
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128276376322048

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