Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity

Detalhes bibliográficos
Autor(a) principal: Yoneyama, Kelly A. G. [UNIFESP]
Data de Publicação: 2006
Outros Autores: Tanaka, Ameria K. [UNIFESP], Silveira, Thais G. V., Takahashi, Helio Kiyoshi [UNIFESP], Straus, Anita Hilda [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1194/jlr.M600285-JLR200
http://repositorio.unifesp.br/handle/11600/29172
Resumo: Detergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4 degrees C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glyco-inositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. in contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100 soluble versus -insoluble fractions at 4 degrees C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-beta-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites.
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spelling Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivityDetergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4 degrees C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glyco-inositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. in contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100 soluble versus -insoluble fractions at 4 degrees C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-beta-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites.Universidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, BrazilUniv Estadual Maringa, Dept Analises Clin, BR-87020900 Maringa, Parana, BrazilUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, BrazilWeb of ScienceAmer Soc Biochemistry Molecular Biology IncUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual de Maringá (UEM)Yoneyama, Kelly A. G. [UNIFESP]Tanaka, Ameria K. [UNIFESP]Silveira, Thais G. V.Takahashi, Helio Kiyoshi [UNIFESP]Straus, Anita Hilda [UNIFESP]2016-01-24T12:41:30Z2016-01-24T12:41:30Z2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2171-2178http://dx.doi.org/10.1194/jlr.M600285-JLR200Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 47, n. 10, p. 2171-2178, 2006.10.1194/jlr.M600285-JLR2000022-2275http://repositorio.unifesp.br/handle/11600/29172WOS:000240847600007engJournal of Lipid Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T10:41:30Zoai:repositorio.unifesp.br/:11600/29172Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:41:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
title Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
spellingShingle Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
Yoneyama, Kelly A. G. [UNIFESP]
title_short Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
title_full Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
title_fullStr Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
title_full_unstemmed Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
title_sort Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
author Yoneyama, Kelly A. G. [UNIFESP]
author_facet Yoneyama, Kelly A. G. [UNIFESP]
Tanaka, Ameria K. [UNIFESP]
Silveira, Thais G. V.
Takahashi, Helio Kiyoshi [UNIFESP]
Straus, Anita Hilda [UNIFESP]
author_role author
author2 Tanaka, Ameria K. [UNIFESP]
Silveira, Thais G. V.
Takahashi, Helio Kiyoshi [UNIFESP]
Straus, Anita Hilda [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Maringá (UEM)
dc.contributor.author.fl_str_mv Yoneyama, Kelly A. G. [UNIFESP]
Tanaka, Ameria K. [UNIFESP]
Silveira, Thais G. V.
Takahashi, Helio Kiyoshi [UNIFESP]
Straus, Anita Hilda [UNIFESP]
description Detergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4 degrees C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glyco-inositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. in contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100 soluble versus -insoluble fractions at 4 degrees C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-beta-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites.
publishDate 2006
dc.date.none.fl_str_mv 2006-10-01
2016-01-24T12:41:30Z
2016-01-24T12:41:30Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1194/jlr.M600285-JLR200
Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 47, n. 10, p. 2171-2178, 2006.
10.1194/jlr.M600285-JLR200
0022-2275
http://repositorio.unifesp.br/handle/11600/29172
WOS:000240847600007
url http://dx.doi.org/10.1194/jlr.M600285-JLR200
http://repositorio.unifesp.br/handle/11600/29172
identifier_str_mv Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 47, n. 10, p. 2171-2178, 2006.
10.1194/jlr.M600285-JLR200
0022-2275
WOS:000240847600007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Lipid Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2171-2178
dc.publisher.none.fl_str_mv Amer Soc Biochemistry Molecular Biology Inc
publisher.none.fl_str_mv Amer Soc Biochemistry Molecular Biology Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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