Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1194/jlr.M600285-JLR200 http://repositorio.unifesp.br/handle/11600/29172 |
Resumo: | Detergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4 degrees C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glyco-inositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. in contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100 soluble versus -insoluble fractions at 4 degrees C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-beta-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites. |
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Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivityDetergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4 degrees C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glyco-inositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. in contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100 soluble versus -insoluble fractions at 4 degrees C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-beta-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites.Universidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, BrazilUniv Estadual Maringa, Dept Analises Clin, BR-87020900 Maringa, Parana, BrazilUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, BrazilWeb of ScienceAmer Soc Biochemistry Molecular Biology IncUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual de Maringá (UEM)Yoneyama, Kelly A. G. [UNIFESP]Tanaka, Ameria K. [UNIFESP]Silveira, Thais G. V.Takahashi, Helio Kiyoshi [UNIFESP]Straus, Anita Hilda [UNIFESP]2016-01-24T12:41:30Z2016-01-24T12:41:30Z2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2171-2178http://dx.doi.org/10.1194/jlr.M600285-JLR200Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 47, n. 10, p. 2171-2178, 2006.10.1194/jlr.M600285-JLR2000022-2275http://repositorio.unifesp.br/handle/11600/29172WOS:000240847600007engJournal of Lipid Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T10:41:30Zoai:repositorio.unifesp.br/:11600/29172Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:41:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
title |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
spellingShingle |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity Yoneyama, Kelly A. G. [UNIFESP] |
title_short |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
title_full |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
title_fullStr |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
title_full_unstemmed |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
title_sort |
Characterization of Leishmania (Viannia) braziliensis membrane microdomains, and their role in macrophage infectivity |
author |
Yoneyama, Kelly A. G. [UNIFESP] |
author_facet |
Yoneyama, Kelly A. G. [UNIFESP] Tanaka, Ameria K. [UNIFESP] Silveira, Thais G. V. Takahashi, Helio Kiyoshi [UNIFESP] Straus, Anita Hilda [UNIFESP] |
author_role |
author |
author2 |
Tanaka, Ameria K. [UNIFESP] Silveira, Thais G. V. Takahashi, Helio Kiyoshi [UNIFESP] Straus, Anita Hilda [UNIFESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Maringá (UEM) |
dc.contributor.author.fl_str_mv |
Yoneyama, Kelly A. G. [UNIFESP] Tanaka, Ameria K. [UNIFESP] Silveira, Thais G. V. Takahashi, Helio Kiyoshi [UNIFESP] Straus, Anita Hilda [UNIFESP] |
description |
Detergent-resistant membranes (DRMs) from Leishmania (Viannia) braziliensis promastigotes, insoluble in 1% Triton X-100 at 4 degrees C, were fractionated by sucrose density gradient ultracentrifugation. They were composed of glyco-inositolphospholipids (GIPLs), inositol phosphorylceramide (IPC), phosphatidylinositol (PI), phosphatidylethanolamine (PE), and sterols. in contrast, 1% Triton X-100-soluble fraction was composed of PE, phosphatidylcholine, phosphatidylserine, PI, IPC, sterol, and lyso-PI. High-performance thin-layer chromatography (HPTLC) immunostaining using monoclonal antibody SST-1 showed that 85% of GIPLs are present in DRMs, and immunoelectron microscopic analysis showed that SST-1-reactive components are located in patches along the parasite surface. No difference in GIPL pattern was observed by HPTLC between Triton X-100 soluble versus -insoluble fractions at 4 degrees C. Analysis of fatty acid composition in DRMs by GC-MS showed the presence of GIPLs containing an alkylacylglycerol, presenting mainly saturated acyl and alkyl chains. DRMs also contained sterol, IPC with saturated fatty acids, PI with at least one saturated acyl chain, and PE with predominantly oleic acid. Promastigotes treated with methyl-beta-cyclodextrin to disrupt lipid microdomains showed significantly lower macrophage infectivity, suggesting a relationship between lipid microdomains and the infectivity of these parasites. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 2016-01-24T12:41:30Z 2016-01-24T12:41:30Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1194/jlr.M600285-JLR200 Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 47, n. 10, p. 2171-2178, 2006. 10.1194/jlr.M600285-JLR200 0022-2275 http://repositorio.unifesp.br/handle/11600/29172 WOS:000240847600007 |
url |
http://dx.doi.org/10.1194/jlr.M600285-JLR200 http://repositorio.unifesp.br/handle/11600/29172 |
identifier_str_mv |
Journal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 47, n. 10, p. 2171-2178, 2006. 10.1194/jlr.M600285-JLR200 0022-2275 WOS:000240847600007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Lipid Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2171-2178 |
dc.publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268364329058304 |