Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0102-865020160010000004 http://repositorio.unifesp.br/handle/11600/49619 |
Resumo: | PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADR |
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Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in ratsAnal CanalAnusImperforateDoxorubicinFolic AcidRatsNeural-Tube DefectsEmbryonic-DevelopmentHindgut DevelopmentMouse ModelSupplementationNotochordEthylenethioureaExpressionPregnancyAnomaliesPURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADRGroup B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p <= 0.05*. RESULTS: 81 fetuses were harvested. The number of fetusesnumber of ARMsmean (Delta%) (+/- SD) were determined to be, respectively: ADR -41[2965%(+/- 37%)] versus FA+ADR - 40[0416%(+/- 36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (mu m) of ASSE (+/- SD) and IE (+/- SD) were measured, respectively: ADR -[25.98(+/- 0.74) and 19.48(+/- 1.68)] versus FA+ADR -[24.74(+/- 0.91) and 24.80(+/- 0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005). CONCLUSIONS: Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced.Postgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo (UNIFESP), BrazilHistology and Structural Biology Division, Department of Morphology, UNIFESP, Sao Paulo-SP, BrazilUniversidade São Judas Tadeu, Sao Paulo-SP, BrazilDepartment of Pediatric Surgery, UNIFESP, Sao Paulo-SP, BrazilPostgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo (UNIFESP), BrazilHistology and Structural Biology Division, Department of Morphology, UNIFESP, Sao Paulo-SP, BrazilDepartment of Pediatric Surgery, UNIFESP, Sao Paulo-SP, BrazilWeb of ScienceActa cirurgica brasileira2019-01-21T10:30:09Z2019-01-21T10:30:09Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion22-27http://dx.doi.org/10.1590/S0102-865020160010000004Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016.10.1590/S0102-865020160010000004S0102-86502016000100022.pdf0102-8650S0102-86502016000100022http://repositorio.unifesp.br/handle/11600/49619WOS:000369657900004engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessFiorindo Faria, Danilo Jose [UNIFESP]Simoes, Manuel de Jesus [UNIFESP]Teixeira, Luciana Cristina [UNIFESP]Pinheiro Faria, Angela TucciSimoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP]Martins, Jose Luiz [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-30T11:04:49Zoai:repositorio.unifesp.br/:11600/49619Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-30T11:04:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| title |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| spellingShingle |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats Fiorindo Faria, Danilo Jose [UNIFESP] Anal Canal Anus Imperforate Doxorubicin Folic Acid RatsNeural-Tube Defects Embryonic-Development Hindgut Development Mouse Model Supplementation Notochord Ethylenethiourea Expression Pregnancy Anomalies |
| title_short |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| title_full |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| title_fullStr |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| title_full_unstemmed |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| title_sort |
Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats |
| author |
Fiorindo Faria, Danilo Jose [UNIFESP] |
| author_facet |
Fiorindo Faria, Danilo Jose [UNIFESP] Simoes, Manuel de Jesus [UNIFESP] Teixeira, Luciana Cristina [UNIFESP] Pinheiro Faria, Angela Tucci Simoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP] Martins, Jose Luiz [UNIFESP] |
| author_role |
author |
| author2 |
Simoes, Manuel de Jesus [UNIFESP] Teixeira, Luciana Cristina [UNIFESP] Pinheiro Faria, Angela Tucci Simoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP] Martins, Jose Luiz [UNIFESP] |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Fiorindo Faria, Danilo Jose [UNIFESP] Simoes, Manuel de Jesus [UNIFESP] Teixeira, Luciana Cristina [UNIFESP] Pinheiro Faria, Angela Tucci Simoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP] Martins, Jose Luiz [UNIFESP] |
| dc.subject.por.fl_str_mv |
Anal Canal Anus Imperforate Doxorubicin Folic Acid RatsNeural-Tube Defects Embryonic-Development Hindgut Development Mouse Model Supplementation Notochord Ethylenethiourea Expression Pregnancy Anomalies |
| topic |
Anal Canal Anus Imperforate Doxorubicin Folic Acid RatsNeural-Tube Defects Embryonic-Development Hindgut Development Mouse Model Supplementation Notochord Ethylenethiourea Expression Pregnancy Anomalies |
| description |
PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADR |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2019-01-21T10:30:09Z 2019-01-21T10:30:09Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-865020160010000004 Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016. 10.1590/S0102-865020160010000004 S0102-86502016000100022.pdf 0102-8650 S0102-86502016000100022 http://repositorio.unifesp.br/handle/11600/49619 WOS:000369657900004 |
| url |
http://dx.doi.org/10.1590/S0102-865020160010000004 http://repositorio.unifesp.br/handle/11600/49619 |
| identifier_str_mv |
Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016. 10.1590/S0102-865020160010000004 S0102-86502016000100022.pdf 0102-8650 S0102-86502016000100022 WOS:000369657900004 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
22-27 |
| dc.publisher.none.fl_str_mv |
Acta cirurgica brasileira |
| publisher.none.fl_str_mv |
Acta cirurgica brasileira |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268452370644992 |