Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82

Detalhes bibliográficos
Autor(a) principal: Manque, Patricio M. [UNIFESP]
Data de Publicação: 2003
Outros Autores: Neira, Ivan [UNIFESP], Atayde, Vanessa [UNIFESP], Cordero, Esteban [UNIFESP], Ferreira, Alice T. [UNIFESP], Silveira, Jose Franco da [UNIFESP], Ramirez, Marcel [UNIFESP], Yoshida, Nobuko [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1128/IAI.71.3.1561-1565.2003
http://repositorio.unifesp.br/handle/11600/27157
Resumo: Metacyclic trypomastigotes of Trypanosoma cruzi express a developmentally regulated 82-kDa surface glycoprotein (gp82) that has been implicated in host cell invasion. gp82-mediated interaction of metacyclic forms with target cells induces in both cells activation of the signal transduction pathways, leading to intracellular Ca2+ mobilization, which is required for parasite internalization. Noninfective epimastigotes do not express detectable levels of gp82 and are unable to induce a Ca2+ response. We stably transfected epimastigotes with a T. cruzi expression vector carrying the metacyclic stage gp82 cDNA. These transfectants produced a functional gp82, which bound to and triggered a Ca2+ response in HeLa cells, in the same manner as the metacyclic trypomastigote gp82. Such properties were not found in epimastigotes transfected with the plasmid vector alone. Epimastigotes expressing gp82 on the surface adhered to HeLa cells but were not internalized. Treatment of gp82-expressing epimastigotes with forskolin, an activator of adenylyl cyclase that increases the metacyclic trypomastigote entry into target cells, did not promote parasite internalization. P175, an intracellular tyrosine phosphorylated protein, which appears to play a role in gp82-dependent signaling cascade in metacyclic forms, was undetectable in epimastigotes, either transfected or not with pTEX-gp82. Overall, our results indicate that gp82 is required but not sufficient for target cell invasion.
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spelling Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82Metacyclic trypomastigotes of Trypanosoma cruzi express a developmentally regulated 82-kDa surface glycoprotein (gp82) that has been implicated in host cell invasion. gp82-mediated interaction of metacyclic forms with target cells induces in both cells activation of the signal transduction pathways, leading to intracellular Ca2+ mobilization, which is required for parasite internalization. Noninfective epimastigotes do not express detectable levels of gp82 and are unable to induce a Ca2+ response. We stably transfected epimastigotes with a T. cruzi expression vector carrying the metacyclic stage gp82 cDNA. These transfectants produced a functional gp82, which bound to and triggered a Ca2+ response in HeLa cells, in the same manner as the metacyclic trypomastigote gp82. Such properties were not found in epimastigotes transfected with the plasmid vector alone. Epimastigotes expressing gp82 on the surface adhered to HeLa cells but were not internalized. Treatment of gp82-expressing epimastigotes with forskolin, an activator of adenylyl cyclase that increases the metacyclic trypomastigote entry into target cells, did not promote parasite internalization. P175, an intracellular tyrosine phosphorylated protein, which appears to play a role in gp82-dependent signaling cascade in metacyclic forms, was undetectable in epimastigotes, either transfected or not with pTEX-gp82. Overall, our results indicate that gp82 is required but not sufficient for target cell invasion.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04023062 São Paulo, BrazilWeb of ScienceAmer Soc MicrobiologyUniversidade Federal de São Paulo (UNIFESP)Manque, Patricio M. [UNIFESP]Neira, Ivan [UNIFESP]Atayde, Vanessa [UNIFESP]Cordero, Esteban [UNIFESP]Ferreira, Alice T. [UNIFESP]Silveira, Jose Franco da [UNIFESP]Ramirez, Marcel [UNIFESP]Yoshida, Nobuko [UNIFESP]2016-01-24T12:33:44Z2016-01-24T12:33:44Z2003-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1561-1565application/pdfhttp://dx.doi.org/10.1128/IAI.71.3.1561-1565.2003Infection and Immunity. Washington: Amer Soc Microbiology, v. 71, n. 3, p. 1561-1565, 2003.10.1128/IAI.71.3.1561-1565.2003WOS000181270900065.pdf0019-9567http://repositorio.unifesp.br/handle/11600/27157WOS:000181270900065engInfection and Immunityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T02:01:58Zoai:repositorio.unifesp.br/:11600/27157Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T02:01:58Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
title Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
spellingShingle Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
Manque, Patricio M. [UNIFESP]
title_short Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
title_full Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
title_fullStr Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
title_full_unstemmed Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
title_sort Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82
author Manque, Patricio M. [UNIFESP]
author_facet Manque, Patricio M. [UNIFESP]
Neira, Ivan [UNIFESP]
Atayde, Vanessa [UNIFESP]
Cordero, Esteban [UNIFESP]
Ferreira, Alice T. [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
Ramirez, Marcel [UNIFESP]
Yoshida, Nobuko [UNIFESP]
author_role author
author2 Neira, Ivan [UNIFESP]
Atayde, Vanessa [UNIFESP]
Cordero, Esteban [UNIFESP]
Ferreira, Alice T. [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
Ramirez, Marcel [UNIFESP]
Yoshida, Nobuko [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Manque, Patricio M. [UNIFESP]
Neira, Ivan [UNIFESP]
Atayde, Vanessa [UNIFESP]
Cordero, Esteban [UNIFESP]
Ferreira, Alice T. [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
Ramirez, Marcel [UNIFESP]
Yoshida, Nobuko [UNIFESP]
description Metacyclic trypomastigotes of Trypanosoma cruzi express a developmentally regulated 82-kDa surface glycoprotein (gp82) that has been implicated in host cell invasion. gp82-mediated interaction of metacyclic forms with target cells induces in both cells activation of the signal transduction pathways, leading to intracellular Ca2+ mobilization, which is required for parasite internalization. Noninfective epimastigotes do not express detectable levels of gp82 and are unable to induce a Ca2+ response. We stably transfected epimastigotes with a T. cruzi expression vector carrying the metacyclic stage gp82 cDNA. These transfectants produced a functional gp82, which bound to and triggered a Ca2+ response in HeLa cells, in the same manner as the metacyclic trypomastigote gp82. Such properties were not found in epimastigotes transfected with the plasmid vector alone. Epimastigotes expressing gp82 on the surface adhered to HeLa cells but were not internalized. Treatment of gp82-expressing epimastigotes with forskolin, an activator of adenylyl cyclase that increases the metacyclic trypomastigote entry into target cells, did not promote parasite internalization. P175, an intracellular tyrosine phosphorylated protein, which appears to play a role in gp82-dependent signaling cascade in metacyclic forms, was undetectable in epimastigotes, either transfected or not with pTEX-gp82. Overall, our results indicate that gp82 is required but not sufficient for target cell invasion.
publishDate 2003
dc.date.none.fl_str_mv 2003-03-01
2016-01-24T12:33:44Z
2016-01-24T12:33:44Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1128/IAI.71.3.1561-1565.2003
Infection and Immunity. Washington: Amer Soc Microbiology, v. 71, n. 3, p. 1561-1565, 2003.
10.1128/IAI.71.3.1561-1565.2003
WOS000181270900065.pdf
0019-9567
http://repositorio.unifesp.br/handle/11600/27157
WOS:000181270900065
url http://dx.doi.org/10.1128/IAI.71.3.1561-1565.2003
http://repositorio.unifesp.br/handle/11600/27157
identifier_str_mv Infection and Immunity. Washington: Amer Soc Microbiology, v. 71, n. 3, p. 1561-1565, 2003.
10.1128/IAI.71.3.1561-1565.2003
WOS000181270900065.pdf
0019-9567
WOS:000181270900065
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Infection and Immunity
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1561-1565
application/pdf
dc.publisher.none.fl_str_mv Amer Soc Microbiology
publisher.none.fl_str_mv Amer Soc Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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