Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor

Detalhes bibliográficos
Autor(a) principal: Carvalho, Lucimeire Nova de [UNIFESP]
Data de Publicação: 2012
Outros Autores: Cristovam, Priscila Cardoso [UNIFESP], Passos, Clévia dos Santos [UNIFESP], Boim, Mirian Aparecida [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1159/000343333
http://repositorio.unifesp.br/handle/11600/34389
Resumo: Background/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, Basel
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spelling Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 ReceptorPregnancyMesangial cellsIntracellular calciumRelaxinAT2 receptorNitric oxideBackground/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, BaselUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao Oswaldo Ramos (FOR)KargerUniversidade Federal de São Paulo (UNIFESP)Carvalho, Lucimeire Nova de [UNIFESP]Cristovam, Priscila Cardoso [UNIFESP]Passos, Clévia dos Santos [UNIFESP]Boim, Mirian Aparecida [UNIFESP]2016-01-24T14:17:38Z2016-01-24T14:17:38Z2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1456-1464application/pdfhttp://dx.doi.org/10.1159/000343333Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012.10.1159/000343333WOS000314149200012.pdf1015-8987http://repositorio.unifesp.br/handle/11600/34389WOS:000314149200012engCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccesshttp://www.karger.com/Services/RightsPermissionsreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T10:02:35Zoai:repositorio.unifesp.br/:11600/34389Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T10:02:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
title Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
spellingShingle Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
Carvalho, Lucimeire Nova de [UNIFESP]
Pregnancy
Mesangial cells
Intracellular calcium
Relaxin
AT2 receptor
Nitric oxide
title_short Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
title_full Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
title_fullStr Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
title_full_unstemmed Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
title_sort Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
author Carvalho, Lucimeire Nova de [UNIFESP]
author_facet Carvalho, Lucimeire Nova de [UNIFESP]
Cristovam, Priscila Cardoso [UNIFESP]
Passos, Clévia dos Santos [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
author_role author
author2 Cristovam, Priscila Cardoso [UNIFESP]
Passos, Clévia dos Santos [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Carvalho, Lucimeire Nova de [UNIFESP]
Cristovam, Priscila Cardoso [UNIFESP]
Passos, Clévia dos Santos [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
dc.subject.por.fl_str_mv Pregnancy
Mesangial cells
Intracellular calcium
Relaxin
AT2 receptor
Nitric oxide
topic Pregnancy
Mesangial cells
Intracellular calcium
Relaxin
AT2 receptor
Nitric oxide
description Background/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, Basel
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2016-01-24T14:17:38Z
2016-01-24T14:17:38Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000343333
Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012.
10.1159/000343333
WOS000314149200012.pdf
1015-8987
http://repositorio.unifesp.br/handle/11600/34389
WOS:000314149200012
url http://dx.doi.org/10.1159/000343333
http://repositorio.unifesp.br/handle/11600/34389
identifier_str_mv Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012.
10.1159/000343333
WOS000314149200012.pdf
1015-8987
WOS:000314149200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.karger.com/Services/RightsPermissions
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.karger.com/Services/RightsPermissions
dc.format.none.fl_str_mv 1456-1464
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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