Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1159/000343333 http://repositorio.unifesp.br/handle/11600/34389 |
Resumo: | Background/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, Basel |
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Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 ReceptorPregnancyMesangial cellsIntracellular calciumRelaxinAT2 receptorNitric oxideBackground/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, BaselUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Renal, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao Oswaldo Ramos (FOR)KargerUniversidade Federal de São Paulo (UNIFESP)Carvalho, Lucimeire Nova de [UNIFESP]Cristovam, Priscila Cardoso [UNIFESP]Passos, Clévia dos Santos [UNIFESP]Boim, Mirian Aparecida [UNIFESP]2016-01-24T14:17:38Z2016-01-24T14:17:38Z2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1456-1464application/pdfhttp://dx.doi.org/10.1159/000343333Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012.10.1159/000343333WOS000314149200012.pdf1015-8987http://repositorio.unifesp.br/handle/11600/34389WOS:000314149200012engCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccesshttp://www.karger.com/Services/RightsPermissionsreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T10:02:35Zoai:repositorio.unifesp.br/:11600/34389Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T10:02:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
title |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
spellingShingle |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor Carvalho, Lucimeire Nova de [UNIFESP] Pregnancy Mesangial cells Intracellular calcium Relaxin AT2 receptor Nitric oxide |
title_short |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
title_full |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
title_fullStr |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
title_full_unstemmed |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
title_sort |
Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor |
author |
Carvalho, Lucimeire Nova de [UNIFESP] |
author_facet |
Carvalho, Lucimeire Nova de [UNIFESP] Cristovam, Priscila Cardoso [UNIFESP] Passos, Clévia dos Santos [UNIFESP] Boim, Mirian Aparecida [UNIFESP] |
author_role |
author |
author2 |
Cristovam, Priscila Cardoso [UNIFESP] Passos, Clévia dos Santos [UNIFESP] Boim, Mirian Aparecida [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Carvalho, Lucimeire Nova de [UNIFESP] Cristovam, Priscila Cardoso [UNIFESP] Passos, Clévia dos Santos [UNIFESP] Boim, Mirian Aparecida [UNIFESP] |
dc.subject.por.fl_str_mv |
Pregnancy Mesangial cells Intracellular calcium Relaxin AT2 receptor Nitric oxide |
topic |
Pregnancy Mesangial cells Intracellular calcium Relaxin AT2 receptor Nitric oxide |
description |
Background/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p<0.05) in the P cells. V cells treated with RLX displayed a similar response to that observed in P cells, suggesting that RLX can modulate the reactivity of the MCs to Ang II. L-NAME and PD123319 did not interfere in this response. Conclusion: Results suggest that RLX is a mediator of the refractoriness of the MCs to Ang II during pregnancy. Copyright (c) 2012 S. Karger AG, Basel |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2016-01-24T14:17:38Z 2016-01-24T14:17:38Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000343333 Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012. 10.1159/000343333 WOS000314149200012.pdf 1015-8987 http://repositorio.unifesp.br/handle/11600/34389 WOS:000314149200012 |
url |
http://dx.doi.org/10.1159/000343333 http://repositorio.unifesp.br/handle/11600/34389 |
identifier_str_mv |
Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 6, p. 1456-1464, 2012. 10.1159/000343333 WOS000314149200012.pdf 1015-8987 WOS:000314149200012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology and Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.karger.com/Services/RightsPermissions |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.karger.com/Services/RightsPermissions |
dc.format.none.fl_str_mv |
1456-1464 application/pdf |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268282946977792 |