Interferon-gamma and tumour necrosis factor-alpha production by CD4(+)T and CD8(+)T lymphocytes in AIDS patients with tuberculosis

• Autor(a) principal: Cunha, RMD
• Data de Publicação: 2005
• Outros Autores: Kallas, Esper Georges [UNIFESP], Rodrigues, D. S., Burattini, Marcelo Nascimento [UNIFESP], Salomão, Reinaldo [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300001062q
• DOI: 10.1111/j.1365-2249.2005.02796.x
• Texto Completo: http://dx.doi.org/10.1111/j.1365-2249.2005.02796.x; http://repositorio.unifesp.br/handle/11600/28314
• topic: AIDS; cytokines; tuberculosis

Tuberculosis (TB) is usually more severe in HIV-infected patients, and the immune derangement found in co-infected patients may differ from that in each isolated disease. Following mitogen stimulation of peripheral blood mononuclear cells (PBMC), interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha production was evaluated in T cells by flow cytometry, and in culture supernatants by enzyme-linked immunosorbent assay (ELISA) in 33 individuals: 11 AIDS patients with tuberculosis, six asymptomatic HIV-1-infected patients, eight patients with tuberculosis and eight healthy controls. the proportion of CD4(+) T lymphocytes expressing IFN-gamma did not differ between the groups, whereas a trend towards increased proportions of TNF-alpha-expression in CD4(+) T cells was observed in the TB compared to the HIV group, while intermediate values were observed in co-infected patients. Detection of IFN-gamma and TNF-alpha in CD8(+) T lymphocytes was higher in TB than in HIV individuals. Co-infected patients presented intermediate values for IFN-gamma, while TNF-alpha detection was similar to that in HIV mono-infection. in conclusion, the proportion of T cells expressing IFN-gamma was relatively preserved in co-infected patients compared to TB patients, while the percentage of T cells expressing TNF-alpha was decreased, mainly in CD8(+) T lymphocytes. However, the marked reduction in T lymphocyte numbers in co-infected patients led to a striking reduction of both cytokines in PBMC supernatants, a finding that is consistent with the impaired response to Mycobacterium tuberculosis.