The effect of a selective cyclooxygenase-2 (COX-2) inhibitor on the proliferation rate of retinoblastoma cell lines

• Autor(a) principal: Souza, J. P. de
• Data de Publicação: 2006
• Outros Autores: Corrêa, Zélia Maria da Silva [UNIFESP], Marshall, J. C., Anteka, E., Coutinho, A. B., Martins, M. C., Burnier, M. N.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1038/sj.eye.6701938; http://repositorio.unifesp.br/handle/11600/28853
• topic: COX-2; retinoblastoma; nepafenac; cell lines; immunocytochemistry

Purpose To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb) cell lines.Methods Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by imunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. the averaged results per condition were recorded. the Student's t-test was used to compare results from the cells cultured with and without amfenac.Result the Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. the two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immucytochemical analysis.Conclusion the selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb.