A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model

Detalhes bibliográficos
Autor(a) principal: Machado, Daniel Escorsim
Data de Publicação: 2010
Outros Autores: Berardo, Plinio Tostes, Landgraf, Richardt Gama [UNIFESP], Fernandes, Patricia Dias, Palmero, Celia, Alves, Leandro Miranda, Abrao, Mauricio Simoes, Nasciutti, Luiz Eurico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.fertnstert.2009.11.037
http://repositorio.unifesp.br/handle/11600/32545
Resumo: Objective: To analyze the antiangiogenic effects of the selective cyclooxygenase-2 (COX-2) inhibitor parecoxib on the growth of endometrial implants in a rat model of peritoneal endometriosis.Design: Pharmacologic interventions in an experimental model of peritoneal endometriosis.Setting: Research laboratory in the Federal University of Rio de Janeiro.Animal(s): Twenty female Sprague-Dawley rats with experimentally induced endometriosis.Intervention(s): After implantation and establishment of autologous endometrium onto the peritoneum abdominal wall, rats were randomized into groups and treated with parecoxib or the vehicle by IM injection for 30 days.Main Outcome Measure(s): Vascular density, the expression of vascular endothelial growth factor (VEGF) and its receptor Flk-1, the distribution of activated macrophages, the expression of COX-2, and the prostaglandin concentration in the endometriotic lesions treated with parecoxib were analyzed.Result(s): the treatment significantly decreased the implant size, and histologic examination indicated mostly atrophy and regression. A reduction in microvessel density and in the number of macrophages, associated with decreased expression of VEGF and Flk-1, also were observed. the treatment group showed a low concentration of prostaglandin E(2).Conclusion(s): These results suggest that the use of COX-2 selective inhibitors could be effective to suppress the establishment and growth of endometriosis, partially through their antiangiogenic activity. (Fertil Steril (R) 2010; 93: 2674-9. (C) 2010 by American Society for Reproductive Medicine.)
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spelling A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat modelAngiogenesisCOX-2 inhibitorendometriosisPGE(2)vascularizationVEGFObjective: To analyze the antiangiogenic effects of the selective cyclooxygenase-2 (COX-2) inhibitor parecoxib on the growth of endometrial implants in a rat model of peritoneal endometriosis.Design: Pharmacologic interventions in an experimental model of peritoneal endometriosis.Setting: Research laboratory in the Federal University of Rio de Janeiro.Animal(s): Twenty female Sprague-Dawley rats with experimentally induced endometriosis.Intervention(s): After implantation and establishment of autologous endometrium onto the peritoneum abdominal wall, rats were randomized into groups and treated with parecoxib or the vehicle by IM injection for 30 days.Main Outcome Measure(s): Vascular density, the expression of vascular endothelial growth factor (VEGF) and its receptor Flk-1, the distribution of activated macrophages, the expression of COX-2, and the prostaglandin concentration in the endometriotic lesions treated with parecoxib were analyzed.Result(s): the treatment significantly decreased the implant size, and histologic examination indicated mostly atrophy and regression. A reduction in microvessel density and in the number of macrophages, associated with decreased expression of VEGF and Flk-1, also were observed. the treatment group showed a low concentration of prostaglandin E(2).Conclusion(s): These results suggest that the use of COX-2 selective inhibitors could be effective to suppress the establishment and growth of endometriosis, partially through their antiangiogenic activity. (Fertil Steril (R) 2010; 93: 2674-9. (C) 2010 by American Society for Reproductive Medicine.)Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Ilha Fundao, Programa Pesquisa Biol Celular & Desenvolvimento, BR-21941590 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Ciencias Biomed, Programa Pesquisa Desenvolvimento Farmacos, BR-21941590 Rio de Janeiro, BrazilUniv São Paulo, Fac Med, Dept Obstet & Ginecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Elsevier B.V.Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Machado, Daniel EscorsimBerardo, Plinio TostesLandgraf, Richardt Gama [UNIFESP]Fernandes, Patricia DiasPalmero, CeliaAlves, Leandro MirandaAbrao, Mauricio SimoesNasciutti, Luiz Eurico2016-01-24T13:59:41Z2016-01-24T13:59:41Z2010-05-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2674-2679http://dx.doi.org/10.1016/j.fertnstert.2009.11.037Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 8, p. 2674-2679, 2010.10.1016/j.fertnstert.2009.11.0370015-0282http://repositorio.unifesp.br/handle/11600/32545WOS:000277608700030engFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T11:59:41Zoai:repositorio.unifesp.br/:11600/32545Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T11:59:41Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
title A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
spellingShingle A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
Machado, Daniel Escorsim
Angiogenesis
COX-2 inhibitor
endometriosis
PGE(2)
vascularization
VEGF
title_short A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
title_full A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
title_fullStr A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
title_full_unstemmed A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
title_sort A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model
author Machado, Daniel Escorsim
author_facet Machado, Daniel Escorsim
Berardo, Plinio Tostes
Landgraf, Richardt Gama [UNIFESP]
Fernandes, Patricia Dias
Palmero, Celia
Alves, Leandro Miranda
Abrao, Mauricio Simoes
Nasciutti, Luiz Eurico
author_role author
author2 Berardo, Plinio Tostes
Landgraf, Richardt Gama [UNIFESP]
Fernandes, Patricia Dias
Palmero, Celia
Alves, Leandro Miranda
Abrao, Mauricio Simoes
Nasciutti, Luiz Eurico
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Machado, Daniel Escorsim
Berardo, Plinio Tostes
Landgraf, Richardt Gama [UNIFESP]
Fernandes, Patricia Dias
Palmero, Celia
Alves, Leandro Miranda
Abrao, Mauricio Simoes
Nasciutti, Luiz Eurico
dc.subject.por.fl_str_mv Angiogenesis
COX-2 inhibitor
endometriosis
PGE(2)
vascularization
VEGF
topic Angiogenesis
COX-2 inhibitor
endometriosis
PGE(2)
vascularization
VEGF
description Objective: To analyze the antiangiogenic effects of the selective cyclooxygenase-2 (COX-2) inhibitor parecoxib on the growth of endometrial implants in a rat model of peritoneal endometriosis.Design: Pharmacologic interventions in an experimental model of peritoneal endometriosis.Setting: Research laboratory in the Federal University of Rio de Janeiro.Animal(s): Twenty female Sprague-Dawley rats with experimentally induced endometriosis.Intervention(s): After implantation and establishment of autologous endometrium onto the peritoneum abdominal wall, rats were randomized into groups and treated with parecoxib or the vehicle by IM injection for 30 days.Main Outcome Measure(s): Vascular density, the expression of vascular endothelial growth factor (VEGF) and its receptor Flk-1, the distribution of activated macrophages, the expression of COX-2, and the prostaglandin concentration in the endometriotic lesions treated with parecoxib were analyzed.Result(s): the treatment significantly decreased the implant size, and histologic examination indicated mostly atrophy and regression. A reduction in microvessel density and in the number of macrophages, associated with decreased expression of VEGF and Flk-1, also were observed. the treatment group showed a low concentration of prostaglandin E(2).Conclusion(s): These results suggest that the use of COX-2 selective inhibitors could be effective to suppress the establishment and growth of endometriosis, partially through their antiangiogenic activity. (Fertil Steril (R) 2010; 93: 2674-9. (C) 2010 by American Society for Reproductive Medicine.)
publishDate 2010
dc.date.none.fl_str_mv 2010-05-15
2016-01-24T13:59:41Z
2016-01-24T13:59:41Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fertnstert.2009.11.037
Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 8, p. 2674-2679, 2010.
10.1016/j.fertnstert.2009.11.037
0015-0282
http://repositorio.unifesp.br/handle/11600/32545
WOS:000277608700030
url http://dx.doi.org/10.1016/j.fertnstert.2009.11.037
http://repositorio.unifesp.br/handle/11600/32545
identifier_str_mv Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 8, p. 2674-2679, 2010.
10.1016/j.fertnstert.2009.11.037
0015-0282
WOS:000277608700030
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fertility and Sterility
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 2674-2679
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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