Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii

Detalhes bibliográficos
Autor(a) principal: Gegembauer, Gregory [UNIFESP]
Data de Publicação: 2014
Outros Autores: Araujo, Leticia Mendes [UNIFESP], Pereira, Edy Firmina, Rodrigues, Anderson Messias [UNIFESP], Paniago, Anamaria Mello Miranda, Hahn, Rosane Christine, Camargo, Zoilo Pires de [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pntd.0002986
http://repositorio.unifesp.br/handle/11600/37903
Resumo: Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
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spelling Serology of Paracoccidioidomycosis Due to Paracoccidioides lutziiParacoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.Universidade Federal de São Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, Disciplina Biol Celular, São Paulo, BrazilUniv Fed Mato Grosso do Sul UFMS, Fac Med FAMED, Campo Grande, MS, BrazilUniv Fed Mato Grosso UFMT, Nucleo Doencas Infecciosas & Trop, Cuiaba, Mato Grosso, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, Disciplina Biol Celular, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2012/06593-0FAPESP: 2009/54024-2Public Library ScienceUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Mato Grosso do Sul (UFMS)Univ Fed Mato Grosso UFMTGegembauer, Gregory [UNIFESP]Araujo, Leticia Mendes [UNIFESP]Pereira, Edy FirminaRodrigues, Anderson Messias [UNIFESP]Paniago, Anamaria Mello MirandaHahn, Rosane ChristineCamargo, Zoilo Pires de [UNIFESP]2016-01-24T14:37:29Z2016-01-24T14:37:29Z2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12application/pdfhttp://dx.doi.org/10.1371/journal.pntd.0002986Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014.10.1371/journal.pntd.0002986WOS000340551500035.pdf1935-2735http://repositorio.unifesp.br/handle/11600/37903WOS:000340551500035engPlos Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T11:52:01Zoai:repositorio.unifesp.br/:11600/37903Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T11:52:01Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
title Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
spellingShingle Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Gegembauer, Gregory [UNIFESP]
title_short Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
title_full Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
title_fullStr Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
title_full_unstemmed Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
title_sort Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
author Gegembauer, Gregory [UNIFESP]
author_facet Gegembauer, Gregory [UNIFESP]
Araujo, Leticia Mendes [UNIFESP]
Pereira, Edy Firmina
Rodrigues, Anderson Messias [UNIFESP]
Paniago, Anamaria Mello Miranda
Hahn, Rosane Christine
Camargo, Zoilo Pires de [UNIFESP]
author_role author
author2 Araujo, Leticia Mendes [UNIFESP]
Pereira, Edy Firmina
Rodrigues, Anderson Messias [UNIFESP]
Paniago, Anamaria Mello Miranda
Hahn, Rosane Christine
Camargo, Zoilo Pires de [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Mato Grosso do Sul (UFMS)
Univ Fed Mato Grosso UFMT
dc.contributor.author.fl_str_mv Gegembauer, Gregory [UNIFESP]
Araujo, Leticia Mendes [UNIFESP]
Pereira, Edy Firmina
Rodrigues, Anderson Messias [UNIFESP]
Paniago, Anamaria Mello Miranda
Hahn, Rosane Christine
Camargo, Zoilo Pires de [UNIFESP]
description Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
2016-01-24T14:37:29Z
2016-01-24T14:37:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0002986
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014.
10.1371/journal.pntd.0002986
WOS000340551500035.pdf
1935-2735
http://repositorio.unifesp.br/handle/11600/37903
WOS:000340551500035
url http://dx.doi.org/10.1371/journal.pntd.0002986
http://repositorio.unifesp.br/handle/11600/37903
identifier_str_mv Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014.
10.1371/journal.pntd.0002986
WOS000340551500035.pdf
1935-2735
WOS:000340551500035
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos Neglected Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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