Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pntd.0002986 http://repositorio.unifesp.br/handle/11600/37903 |
Resumo: | Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests. |
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Serology of Paracoccidioidomycosis Due to Paracoccidioides lutziiParacoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.Universidade Federal de São Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, Disciplina Biol Celular, São Paulo, BrazilUniv Fed Mato Grosso do Sul UFMS, Fac Med FAMED, Campo Grande, MS, BrazilUniv Fed Mato Grosso UFMT, Nucleo Doencas Infecciosas & Trop, Cuiaba, Mato Grosso, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, Disciplina Biol Celular, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2012/06593-0FAPESP: 2009/54024-2Public Library ScienceUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Mato Grosso do Sul (UFMS)Univ Fed Mato Grosso UFMTGegembauer, Gregory [UNIFESP]Araujo, Leticia Mendes [UNIFESP]Pereira, Edy FirminaRodrigues, Anderson Messias [UNIFESP]Paniago, Anamaria Mello MirandaHahn, Rosane ChristineCamargo, Zoilo Pires de [UNIFESP]2016-01-24T14:37:29Z2016-01-24T14:37:29Z2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12application/pdfhttp://dx.doi.org/10.1371/journal.pntd.0002986Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014.10.1371/journal.pntd.0002986WOS000340551500035.pdf1935-2735http://repositorio.unifesp.br/handle/11600/37903WOS:000340551500035engPlos Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T11:52:01Zoai:repositorio.unifesp.br/:11600/37903Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T11:52:01Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
title |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
spellingShingle |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii Gegembauer, Gregory [UNIFESP] |
title_short |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
title_full |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
title_fullStr |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
title_full_unstemmed |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
title_sort |
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
author |
Gegembauer, Gregory [UNIFESP] |
author_facet |
Gegembauer, Gregory [UNIFESP] Araujo, Leticia Mendes [UNIFESP] Pereira, Edy Firmina Rodrigues, Anderson Messias [UNIFESP] Paniago, Anamaria Mello Miranda Hahn, Rosane Christine Camargo, Zoilo Pires de [UNIFESP] |
author_role |
author |
author2 |
Araujo, Leticia Mendes [UNIFESP] Pereira, Edy Firmina Rodrigues, Anderson Messias [UNIFESP] Paniago, Anamaria Mello Miranda Hahn, Rosane Christine Camargo, Zoilo Pires de [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal de Mato Grosso do Sul (UFMS) Univ Fed Mato Grosso UFMT |
dc.contributor.author.fl_str_mv |
Gegembauer, Gregory [UNIFESP] Araujo, Leticia Mendes [UNIFESP] Pereira, Edy Firmina Rodrigues, Anderson Messias [UNIFESP] Paniago, Anamaria Mello Miranda Hahn, Rosane Christine Camargo, Zoilo Pires de [UNIFESP] |
description |
Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. in western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07-01 2016-01-24T14:37:29Z 2016-01-24T14:37:29Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pntd.0002986 Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014. 10.1371/journal.pntd.0002986 WOS000340551500035.pdf 1935-2735 http://repositorio.unifesp.br/handle/11600/37903 WOS:000340551500035 |
url |
http://dx.doi.org/10.1371/journal.pntd.0002986 http://repositorio.unifesp.br/handle/11600/37903 |
identifier_str_mv |
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 8, n. 7, 12 p., 2014. 10.1371/journal.pntd.0002986 WOS000340551500035.pdf 1935-2735 WOS:000340551500035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos Neglected Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268443104378880 |